Suppressions of Serotonin-Induced Increased Vascular Permeability and Leukocyte Infiltration byBixa orellanaLeaf Extract

Abstract

The aim of the present study was to evaluate the anti-inflammatory activities of aqueous extract of Bixa orellana (AEBO) leaves and its possible mechanisms in animal models. The anti-inflammatory activity of the extract was evaluated using serotonin-induced rat paw edema, increased peritoneal vascular permeability, and leukocyte infiltrations in an air-pouch model. Nitric oxide (NO), indicated by the sum of nitrites and nitrates, and vascular growth endothelial growth factor (VEGF) were measured in paw tissues of rats to determine their involvement in the regulation of increased permeability. Pretreatments with AEBO (50 and 150 mg kg−1) prior to serotonin inductions resulted in maximum inhibitions of 56.2% of paw volume, 45.7% of Evans blue dye leakage in the peritoneal vascular permeability model, and 83.9% of leukocyte infiltration in the air-pouch model. 57.2% maximum inhibition of NO and 27% of VEGF formations in rats' paws were observed with AEBO at the dose of 150 mg kg−1. Pharmacological screening of the extract showed significant (P < 0.05) anti-inflammatory activity, indicated by the suppressions of increased vascular permeability and leukocyte infiltration. The inhibitions of these inflammatory events are probably mediated via inhibition of NO and VEGF formation and release.

Hydroalcoholic Extract and Ethyl Acetate Fraction of Bixa orellana Leaves Decrease the Inflammatory Response to Mycobacterium abscessus Subsp. massiliense

“…The antimicrobial action [ 16 ] and antioxidant activity [ 17 ] of the methanolic extract of B. orellana seeds and leaves against Gram-positive bacteria (e.g., S. mutans and S. sanguinis ) have previously been described. In addition, the antimalarial activity of essential oils and root extracts [ 18 ] and the anti-inflammatory effects of aqueous extracts of the leaves [ 19 , 20 ] have been also reported.…”

Anti-Inflammatory Effect of <i>o</i>-Vanillic Acid on Lipopolysaccharide-Stimulated Macrophages and Inflammation Models

“…Acetic acid causes peritoneal inflammation and induces an increase in PGE 2 and PGF 2a in the peritoneal fluid [43]. This capillary permeability assay is a typical model for acute phase of inflammation, where mediators of inflammation released following stimulation lead to the dilation of both arterioles and venules and increase capillary permeability [24]. The inflammatory response also induces the recruitment of leukocytes in response to inflammatory mediators including cytokines in tissue injury.…”

“…The inflammatory response is characterized by the release of various mediators, such as histamine, serotonin, bradykinin, and prostaglandin, which results in capillary permeability and leukocyte migration at the inflammation site [23,24]. To study the effect of o-VA in vivo, acetic acid-induced capillary permeability and zymosan-induced leukocyte migration models were used.…”

Interfacial Tension; a Stabilizing Factor for Janus Emulsions of Silicone Bixa Orellana Oils

“…Since these emulsions may significantly reduce the complex formulation efforts for pharmaceutical emulsions [28], the authors realized the importance of presenting a fundamental examination of such emulsions to a wider audience. So the vegetable oil, Bixa Orellana, was chosen against its obvious pharmaceutical activity [28][29][30] and combined with a silicone oil. The topology modification was studied during the combined creaming/coalescence process and, in addition, the time dependent behavior was investigated for an emulsion, for which the creaming had been arrested by addition of a polymer.…”