Suppression of tumour growth by orally administered osteopontin is accompanied by alterations in tumour blood vessels

Rittling, Susan R.; Wejse, Peter Langborg; Yagiz, Kadir; Warot, G A; Hui, Tommy

doi:10.1038/bjc.2014.10

Cited by 18 publications

(19 citation statements)

References 50 publications

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“…However, these sites are most likely protected by nearby posttranslational modifications such as phosphorylations, and as shown in this study, glycosylated OPN has been shown to resist proteolysis with neonatal gastric aspirates at pH 3.0 for 1 h at 37°C (Chatterton et al, 2004). As well, OPN has been shown to resist digestion in vivo in mice fed OPN by gavage or solubilized in chocolate milk (da Silva et al, 2009;Rittling et al, 2014). Bovine OPN was also detected in the plasma of human infants fed formula supplemented with bovine milk OPN (Jiang and Lönnerdal, 2019).…”

Section: Discussionsupporting

confidence: 56%

Milk osteopontin retains integrin-binding activity after in vitro gastrointestinal transit

Christensen

Karlsen

Jørgensen

et al. 2020

Journal of Dairy Science

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Osteopontin (OPN) is a multifunctional protein highly expressed in milk, where it is hypothesized to be involved in immunological signaling via the conserved Arg-Gly-Asp (RGD) integrin-binding sequence. Intervention studies have indicated beneficial effects of orally administered OPN in animal and human infants, but the mechanisms underlying these effects are not well described. To induce physiological effects, OPN must resist gastrointestinal transit in a bioactive form. In this study, we subjected bovine milk OPN to in vitro gastrointestinal transit, and characterized the generated fragments using monoclonal antibody and mass spectrometric analyses. We found that the fragment Trp 27 -Phe 151 containing the integrin-binding RGD sequence resisted in vitro gastric digestion. This resistance was dependent on glycosylation of threonine residues near the integrin-binding sequence in both human and bovine milk OPN. Furthermore, the fragment Trp 27 -Phe 151 retained the ability to interact with integrins in an RGD-dependent process. These results suggest a mechanism for how ingested milk OPN can induce physiological effects via integrin signaling in the intestine.

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Section: Discussionsupporting

confidence: 56%

Milk osteopontin retains integrin-binding activity after in vitro gastrointestinal transit

Christensen

Karlsen

Jørgensen

et al. 2020

Journal of Dairy Science

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“…While trust was not specifically addressed in the focus groups, some participants mentioned that returning study findings could increase one's likelihood to recommend participating in research. Given the multitude of well-known, documented disparities in research participation [22][23][24][25][26][27], disseminating research results to participants could be one meaningful way to build trust and encourage future research participation [6,9,10,28,29]. Future research should assess how returning study findings impacts participants' trust and likelihood of participating in future research studies.…”

Section: Discussionmentioning

confidence: 99%

Assessing research participant preferences for receiving study results

Cook

Mayers

Goggins

et al. 2019

J. Clin. Trans. Sci.

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AbstractIntroduction:Dissemination of results to research participants is largely missing from the practices of most researchers. Few resources exist that describe best practices for disseminating information to this important stakeholder group.Methods:Four focus groups were conducted with a diverse group of individuals. All participants were part of a Patient-Centered Outcomes Research Institute-funded survey study. Focus groups aimed to identify participants’ preferences about receiving research results and their reactions to three different dissemination platforms.Results:Four focus groups with 37 participants were conducted, including: (1) adults with one comorbidity, at least a college education, and high socioeconomic status (SES); (2) adults with one comorbidity, less than a college education, and lower SES; (3) adults with low health literacy and/or numeracy; and (4) Black or African American adults. Participants discussed their preferences for research results delivery and how each of the platforms met those preferences. This included information needs as they relate to content and scope, including a desire to receive both individual and aggregate results, and study summaries. Email, paper, and website were all popular avenues of presentation. Some desired a written summary, and others preferred videos or visual graphics. Importantly, participants emphasized the desire for having a choice in the timing, frequency, and types of results.Conclusion:Research participants prefer to receive research results, including study impact and key findings, disseminated to them in an engaging format that allows choice of when and how the information is presented. The results encourage new standards whereby research participants are considered a critical stakeholder group.

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“…In vitro experiments have indicated that human and bovine milk OPN are in part resistant to proteolysis in the infant intestinal tract, which makes OPN a potentially bioactive component of human milk [ 108 ]. In animal studies after oral application of OPN some proteolytic cleavage products or even intact OPN could be detected in plasma using antibodies raised against OPN [ 109 , 110 ] and these peptides show biological effects [ 110 ].…”

Section: Human Milk Proteins With Bioactivitymentioning

confidence: 99%

Benefits of Lactoferrin, Osteopontin and Milk Fat Globule Membranes for Infants

Demmelmair¹,

Prell²,

Timby

et al. 2017

Nutrients

120

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The provision of essential and non-essential amino acids for breast-fed infants is the major function of milk proteins. In addition, breast-fed infants might benefit from bioactivities of milk proteins, which are exhibited in the intestine during the digestive phase and by absorption of intact proteins or derived peptides. For lactoferrin, osteopontin and milk fat globule membrane proteins/lipids, which have not until recently been included in substantial amounts in infant formulas, in vitro experiments and animal models provide a convincing base of evidence for bioactivities, which contribute to the protection of the infant from pathogens, improve nutrient absorption, support the development of the immune system and provide components for optimal neurodevelopment. Technologies have become available to obtain these compounds from cow´s milk and the bovine compounds also exhibit bioactivities in humans. Randomized clinical trials with experimental infant formulas incorporating lactoferrin, osteopontin, or milk fat globule membranes have already provided some evidence for clinical benefits. This review aims to compare findings from laboratory and animal experiments with outcomes of clinical studies. There is good justification from basic science and there are promising results from clinical studies for beneficial effects of lactoferrin, osteopontin and the milk fat globule membrane complex of proteins and lipids. Further studies should ideally be adequately powered to investigate effects on clinically relevant endpoints in healthy term infants.

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Suppression of tumour growth by orally administered osteopontin is accompanied by alterations in tumour blood vessels

Cited by 18 publications

References 50 publications

Milk osteopontin retains integrin-binding activity after in vitro gastrointestinal transit

Milk osteopontin retains integrin-binding activity after in vitro gastrointestinal transit

Assessing research participant preferences for receiving study results

Benefits of Lactoferrin, Osteopontin and Milk Fat Globule Membranes for Infants

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