Suppression of tumor-induced angiogenesis by taspine isolated from Radix et Rhizoma Leonticis and its mechanism of action in vitro

Zhang, Yanmin; He, Langchong; Liang, Meng; Luo, Wenjuan; Xu, Xuemei

doi:10.1016/j.canlet.2007.11.035

Cited by 35 publications

(21 citation statements)

References 41 publications

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“…Taspine was able to inhibit chicken chorioallantoic membrane angiogenesis through interfering with the proliferation and migration of endothelial cells in a dose-dependent manner [57]. The exact mechanism [15] has been further demonstrated, suggesting that VEGF and bFGF secretion were downregulated by taspine in human non-small cell lung cancer cell (A549 cell) and human umbilical vein endothelial cells (HUVECs), confirmed by the decreased mRNA level of VEGF and Flk-1/KDR after taspine treatment in HUVECs. The molecular mechanisms of taspine on tumor angiogenic inhibition have been further studied in vitro [58], which indicated that taspine significantly inhibited cell proliferation of HUVECs induced by VEGF165 via decreasing Akt and Erk1/2 activities except decreasing VEGF level.…”

Section: Bioactivitymentioning

confidence: 99%

“…Its roots and rhizomes have been used as folk medicine to treat external injuries, irregular-menses, and stomach-ache due to its strong and wide biological activities [10]. Modern pharmacological studies have demonstrated that alkaloids and triterpence saponins are responsible for its major biological function as an anti-inflammatory [11], analgesic [12], antioxidant [13], antibacterial [11], antiacetylcholinesterase [14], and antitumor [15, 16]. Taspine, a lead compound in anticancer agent development [17, 18], was firstly screened to possess obvious effect on tumor angiogenesis and human epidermal growth factor receptor by using cell membrane chromatography from the C. robustum [19].…”

Section: Introductionmentioning

confidence: 99%

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Genus Caulophyllum: An Overview of Chemistry and Bioactivity

Xia

Liang

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Recently, some promising advances have been achieved in understanding the chemistry, pharmacology, and action mechanisms of constituents from genus Caulophyllum. Despite this, there is to date no systematic review of those of genus Caulophyllum. This review covers naturally occurring alkaloids and saponins and those resulting from synthetic novel taspine derivatives. The paper further discussed several aspects of this genus, including pharmacological properties, mechanisms of action, pharmacokinetics, and cell membrane chromatography for activity screening. The aim of this paper is to provide a point of reference for pharmaceutical researchers to develop new drugs from constituents of Caulophyllum plants.

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Section: Bioactivitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genus Caulophyllum: An Overview of Chemistry and Bioactivity

Xia

Liang

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…The 48-hr cultured medium was collected. VEGF protein concentrations were quantitatively measured by a commercially available VEGF-ELISA kit at 450 nm [23]. …”

Section: Methodsmentioning

confidence: 99%

Brucine suppresses colon cancer cells growthviamediatingKDRsignalling pathway

Luo

Wang

Zheng

et al. 2013

J Cellular Molecular Medi

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Angiogenesis plays an important role in colon cancer development. This study aimed to demonstrate the effect of brucine on tumour angiogenesis and its mechanism of action. The anti-angiogenic effect was evaluated on the chicken chorioallantoic membrane (CAM) model and tube formation. The mechanism was demonstrated through detecting mRNA and protein expressions of VEGFR2 (KDR), PKCα, PLCγ and Raf1 by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot (WB), as well as expressions of VEGF and PKCβ and mTOR by ELISA and WB. The results showed that brucine significantly reduced angiogenesis of CAM and tube formation, inhibited the VEGF secretion and mTOR expression in LoVo cell and down-regulated the mRNA and phosphorylation protein expressions of KDR, PKCα, PLCγ and Raf1. In addition, the effects of brucine on KDR kinase activity, viability of LoVo cell and gene knockdown cell were detected with the Lance™ assay, WST-1 assay and instantaneous siRNA. Compared to that of normal LoVo cells, the inhibition on proliferation of knockdown cells by brucine decreased significantly. These results suggest that brucine could inhibit angiogenesis and be a useful therapeutic candidate for colon cancer intervention.

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“…Briefly, exponentially growing U251 cells were harvested and plated in 96-well plates at a concentration of 5 × 10 3 cells/well. After 24 h incubation at 37 °C, cells were treated with new compounds at various concentrations for 72 h. Then, 20 μL of MTT (5 mg/mL) was added to each well and incubated at 37 °C for 4 h. After the supernatant was discarded, 150 μL k ′ = (t R − t 0 )/t 0 of DMSO was added to each well, and the optical density of cells was determined with a microplate reader (Bio-Rad Instruments, USA) at 490 nm and expressed as absorbance values [20].…”

Section: Cell Viability Assaymentioning

confidence: 99%