Suppression of tumor-induced angiogenesis by Brazilian propolis: Major component artepillin C inhibits in vitro tube formation and endothelial cell proliferation

Ahn, Mok-Ryeon; Kunimasa, Kazuhiro; Ohta, Toshiro; Kumazawa, Shigenori; Kamihira, Miya; Kaji, Koichi; Uto, Yoshihiro; Hori, Hitoshi; Nagasawa, Hideko; Nakayama, Tsutomu

doi:10.1016/j.canlet.2006.12.039

Cited by 130 publications

(116 citation statements)

References 42 publications

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“…16 chemical ingredients were identified in propolis group 12, and the major compound in the extract was artepillin C (3,5-diprenyl-4hydroxycinamic acid), which was reported to inhibit lipid peroxidation and the development of pulmonary cancers (KIMOTO et al, 2001). In addition, artepillin C has a tumor suppressing effect not only by directly inhibiting tumor cell growth, but also by inhibiting angiogenesis (AHN et al, 2007). With regard to propolis group 13, 18 chemical ingredients were identified, and the major compound in the extract was formononetin, which is an isoflavonoid, including daidzein and biochanin A.…”

Section: Propolis Groups 12 and 13mentioning

confidence: 99%

Comparative antiproliferation of human prostate cancer cells by ethanolic extracts of two groups of Brazilian propolis

Moraes

Daugsch

et al. 2010

Ciênc. Tecnol. Aliment.

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Section: Propolis Groups 12 and 13mentioning

confidence: 99%

Comparative antiproliferation of human prostate cancer cells by ethanolic extracts of two groups of Brazilian propolis

Moraes

Daugsch

et al. 2010

Ciênc. Tecnol. Aliment.

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“…The discovery of the anticarcinogenic effects of propolis encouraged efforts to identify, isolate and synthesize constituents of propolis and to study their effects and mechanisms of action, including artepillin C 11 , caffeic acid 12 , caffeic acid phenethyl ester 13 , and in the present study, we used an in vivo model to evaluate the effect of intragastric administration of green propolis (in the form of WSDP) on angiogenesis in BBN-induced rat bladder cancer.…”

Section: Introductionmentioning

confidence: 99%

Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

Dornelas¹,

Fechine-Jamacaru²,

Albuquerque³

et al. 2012

Acta Cir. Bras.

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PURPOSE:To determine the effects of water-soluble derivative of green propolis in bladder cancer angiogenesis in rats given N-butyl-(-4-hydroxybutyl) nitrosamine (BBN). METHODS:Nine groups were established, where six of them (Groups 1 to 6), the animals received 0.05% BBN in their drinking water for 14 weeks. From the 32nd to the 40th week, Groups 1, 2, 3 and 4 were treated respectively with water, L-lysine (300 mg/kg/ day), celecoxib (30 mg/kg/day) and propolis (300 mg/kg/day). Groups 5 and 6 were given propolis and L-lysine from the 1st to the 40th week (150 mg/kg/day). Microvascular density was determined by histological sections stained for the marker CD-31 and analyzed with specific software. RESULTS:The microvascular density in bladder carcinomas was lower (p<0.01) in rats receiving propolis than in controls given carcinogen only. On the other hand, the microvascular density of tumors in rats receiving carcinogen and L-lysine for 40 weeks from the beginning of carcinogen treatment was significantly higher (p<0.01) than in the corresponding controls. -Acta Cirúrgica Brasileira -Vol. 27 (8) 2012de beber por 14 semanas. Na 32ª semana das 40 semanas, os grupos 1, 2, 3 e 4 foram tratados respectivamente com água, L lisina (300 mg/kg/dia), celecoxibe (30 mg/kg/dia) e própolis (300 mg/kg/dia). Os grupos 5 e 6 receberam própolis e L lisina da 1ª a 40ª semana (150 mg/ kg/dia). A densidade microvascular foi determinada por cortes histológicos corados pelo CD-31 e analisados por programa de computador específico. RESULTADOS:A densidade microvascular em carcinomas de bexiga foi menor com p<0,01 nos ratos que receberam própolis do que nos carcinomas do grupo controle que recebeu apenas carcinógeno. Por outro lado, a densidade microvascular de tumores de ratos que receberam carcinógeno e L-Lisina por 40 semanas desde o início do carcinógeno foi significantemente maior com p<0,01 que a densidade microvascular dos tumores de seu respectivo grupo controle. CONCLUSÃO:A própolis verde solúvel em água inibiu a angiogênese em câncer de bexiga induzido pelo BBN, enquanto a L-lisina estimulou a angiogênese quando iniciada juntamente com o BBN.

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“…These compounds, originating from multiple natural product classes, such as flavonoids, phenylpropanoids and polyketides, often possess anti-microbial, anti-oxidant, anti-inflammatory, anti-viral or anti-cancer activities. [1][2][3][4] For example, prenylated phenylpropanoids, drupanin, artepillin C and baccharin, which are p-coumaric acid derivatives with one or two prenyl groups, have been shown to induce an apoptotic event in human leukemia cell line HL60 and colon cancer cell line SW480. 5 Moreover, it has been reported that the oral administration of these prenylated phenylpropanoids to mice allografted with sarcoma S-180 causes a significant reduction in tumor growth.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, given their enhanced and distinct activities, prenylated flavonoids and phenylpropanoids show promise as lead compounds for the development of nutraceuticals in plants and as new pharmacological agents for the treatment of human diseases. [1][2][3][4] However, as prenylated compounds often exist at trace levels in natural sources, they are seldom amenable to cost-effective synthesis.…”

Section: Introductionmentioning

confidence: 99%

NovQ is a prenyltransferase capable of catalyzing the addition of a dimethylallyl group to both phenylpropanoids and flavonoids

et al. 2009

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NovQ is a member of a recently identified CloQ/NphB class of prenyltransferases. Although NphB has been well characterized as a prenyltransferase with flexibility against aromatic substrates, few studies have been carried out on characterization of NovQ. Hence, in this study, we investigate the kinetics, substrate specificity and regiospecificity of NovQ. The corresponding novQ gene was cloned from Streptomyces niveus, which produces an aminocoumarin antibiotic, novobiocin. Recombinant NovQ was overexpressed in Escherichia coli and purified to homogeneity. The purified enzyme was a soluble monomeric 40-kDa protein that catalyzed the transfer of a dimethylallyl group to 4-hydroxyphenylpyruvate (4-HPP) independently of divalent cations to yield 3-dimethylallyl-4-HPP, an intermediate of novobiocin. Steady-state kinetic constants for NovQ with the two substrates, 4-HPP and dimethylallyl diphosphate, were also calculated. In addition to the prenylation of 4-HPP, NovQ catalyzed carbon-carbon-based and carbon-oxygen-based prenylations of a diverse collection of phenylpropanoids, flavonoids and dihydroxynaphthalenes. Despite its catalytic promiscuity, the NovQ-catalyzed prenylation occurred in a regiospecific manner. NovQ is the first reported prenyltransferase capable of catalyzing the transfer of a dimethylallyl group to both phenylpropanoids, such as p-coumaric acid and caffeic acid, and the B-ring of flavonoids. This study shows that NovQ can serve as a useful biocatalyst for the synthesis of prenylated phenylpropanoids and prenylated flavonoids.

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Suppression of tumor-induced angiogenesis by Brazilian propolis: Major component artepillin C inhibits in vitro tube formation and endothelial cell proliferation

Cited by 130 publications

References 42 publications

Comparative antiproliferation of human prostate cancer cells by ethanolic extracts of two groups of Brazilian propolis

Comparative antiproliferation of human prostate cancer cells by ethanolic extracts of two groups of Brazilian propolis

Angiogenesis inhibition by green propolis and the angiogenic effect of L-lysine on bladder cancer in rats

NovQ is a prenyltransferase capable of catalyzing the addition of a dimethylallyl group to both phenylpropanoids and flavonoids

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