Suppression of tumor growth in H-ras12V liver cancer mice by delivery of programmed cell death protein 4 using galactosylated poly(ethylene glycol)-chitosan-graft-spermine

Kim, Jihye; Minai-Tehrani, Arash; Kim, You-Kyoung; Shin, Ji‐Young; Hong, Sun; Kim, Hye-Joon; Lee, Hee-Do; Chang, Simyung; Yu, Kyeong-Nam; Bang, Yongbin; Cho, Chong‐Su; Yoon, Tae‐Jong; Yu, Dae‐Yeul; Jiang, Hu‐Lin; Cho, Myung‐Haing

doi:10.1016/j.biomaterials.2011.11.024

Cited by 28 publications

(7 citation statements)

References 34 publications

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“…To further check, verify therapeutic effect of SPE‐ alt ‐PEG for lung cancer gene therapy, Pdcd4 gene was used as a therapeutic gene. It has already been reported that PDCD4 is a protein translation inhibitor that prevents tumorigenesis and tumor progression . As expected, over 1 mm tumor [Fig.…”

Section: Resultssupporting

confidence: 74%

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Spermine‐alt‐poly(ethylene glycol) polyspermine as a safe and efficient aerosol gene carrier for lung cancer therapy

Kim

Cho

et al. 2013

J Biomedical Materials Res

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The clinical success of gene therapy critically depends upon the safety and efficiency of delivery system used. Although polyethylenimine (PEI) has been commonly used as an efficient cationic polymeric gene carrier due to its high transfection efficiency, its cytotoxicity and nondegradability limit the polymer's therapeutic applications in clinical trials. In this study, biocompatible polyspermine based on spermine (SPE) and poly(ethylene glycol) (PEG) diacrylate (SPE-alt-PEG) was synthesized using a Michael-type addition reaction, and its ability as an alternative gene carrier for lung cancer therapy was evaluated. SPE-alt-PEG polyspermine was complexed with plasmid DNA, and the resulting complexes were characterized by particle size and surface charge by dynamic light scattering, complex formation and DNA protection ability by gel retardation, and complex shape by energy-filtering transmission electron microscopy. The SPE-alt-PEG copolymer showed low cytotoxicity, and SPE-alt-PEG/DNA complexes showed efficacious transfection efficiency compared with 25 kDa PEI (PEI 25K). Also SPE-alt-PEG/GFP complexes were efficiently transferred into the lungs after aerosol administration without toxicity, and delivery of Pdcd4 gene as a therapeutic gene with SPE-alt-PEG polyspermine greatly reduced tumor size as well as tumor numbers in K-ras(LA1) lung cancer model mice compared relative to the effect observed for PEI 25K. These results suggest that SPE-alt-PEG has potential as a gene carrier for lung cancer gene therapy.

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Section: Resultssupporting

confidence: 74%

“…It has already been reported that PDCD4 is a protein translation inhibitor that prevents tumorigenesis and tumor progression. 23,37 As expected, over 1 mm tumor [ Fig. 5(B)] and total tumor numbers [ Fig.…”

Section: In Vitro Transfection Studiessupporting

confidence: 76%

Spermine‐alt‐poly(ethylene glycol) polyspermine as a safe and efficient aerosol gene carrier for lung cancer therapy

Kim

Cho

et al. 2013

J Biomedical Materials Res

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“…Chitosan is a modified natural carbohydrate polymer prepared by the partial N -deacetylation of the crustacean-derived natural biopolymer, chitin, and it has been proposed as an alternative, biocompatible cationic polymers that are suitable for mucosal drug and vaccine delivery [9–13] and gene delivery [14–21]. Chemically modified chitosans have great utility in controlled release and targeting studies of almost all classes of bioactive molecules.…”

Section: Introductionmentioning

confidence: 99%

Galactosylated Chitosan Oligosaccharide Nanoparticles for Hepatocellular Carcinoma Cell-Targeted Delivery of Adenosine Triphosphate

Zhu

et al. 2013

IJMS

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Nanoparticles composed of galactosylated chitosan oligosaccharide (Gal-CSO) and adenosine triphosphate (ATP) were prepared for hepatocellular carcinoma cell-specific uptake, and the characteristics of Gal-CSO/ATP nanoparticles were evaluated. CSO/ATP nanoparticles were prepared as a control. The average diameter and zeta potential of Gal-CSO/ATP nanoparticles were 51.03 ± 3.26 nm and 30.50 ± 1.25 mV, respectively, suggesting suitable properties for a drug delivery system. Subsequently, the cytotoxicity of Gal-CSO/ATP nanoparticles were examined by the methyl tetrazolium (MTT) assay, and the half maximal inhibitory concentration (IC50) values were calculated with HepG2 (human hepatocellular carcinoma cell line) cells. The results showed that the cytotoxic effect of nanoparticles on HepG2 cells was low. In the meantime, it was also found that the Gal-CSO/ATP nanoparticles could be uptaken by HepG2 cells, due to expression of the asialoglycoprotein receptor (ASGP-R) on their surfaces. The presented results indicate that the Gal-CSO nanoparticles might be very attractive to be used as an intracellular drug delivery carrier for hepatocellular carcinoma cell targeting, thus warranting further in vivo or clinical investigations.

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“…46 In this study, positively charged CASF could form complexes with negatively charged pDNA via electrostatic interaction ( Figure 4A). In the preparation process of CASF/pDNA nanoparticles, a mixture of the ASF/ spermine/ASF-spermine conjugate/pDNA was probably formed; however, the resulting nanoparticles were mainly CASF/pDNA in our experimental system.…”

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confidence: 59%

Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier

Yu¹,

Hu²,

Li³

et al. 2016

IJN

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The development of a novel cationized polymer used as a gene delivery carrier that can conveniently and effectively transfect cells resulting in a stably expressed target gene remains a challenge. Antheraea pernyi silk fibroin (ASF) is a cytocompatible and biodegradable natural polymer, and it possesses Arg–Gly–Asp sequences but a negative charge. In order to render ASF amenable to packaging plasmid DNA (pDNA), spermine was used to modify ASF to synthesize cationized ASF (CASF), which was used as a gene delivery carrier. CASF was characterized using trinitrobenzene sulfonic acid assay, the zeta potential determination, and a Fourier transform infrared analysis, and the results of these characterizations indicated that the –NH 2 in spermine effectively reacts with the –COOH in the side chains of ASF. Spermine grafted to the side chains of ASF resulted in the conversion of the negative charge of ASF to a positive charge. CASF packaged pDNA and formed CASF/pDNA complexes, which exhibited spherical morphology with average particle sizes of 215–281 nm and zeta potential of approximately +3.0 mV to +3.2 mV. The results of the MTT assay, confocal laser scanning microscopy, and flow cytometry analysis in a human endothelial cell line revealed that CASF/pDNA complexes exhibited lower cytotoxicity and higher transfection efficiency compared to the pDNA complexes of polyethyleneimine. These results indicate that our synthesized CASF, a cationized polymer, is a potential gene delivery carrier with the advantages of biodegradability and low cytotoxicity.

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Suppression of tumor growth in H-ras12V liver cancer mice by delivery of programmed cell death protein 4 using galactosylated poly(ethylene glycol)-chitosan-graft-spermine

Cited by 28 publications

References 34 publications

Spermine‐alt‐poly(ethylene glycol) polyspermine as a safe and efficient aerosol gene carrier for lung cancer therapy

Spermine‐alt‐poly(ethylene glycol) polyspermine as a safe and efficient aerosol gene carrier for lung cancer therapy

Galactosylated Chitosan Oligosaccharide Nanoparticles for Hepatocellular Carcinoma Cell-Targeted Delivery of Adenosine Triphosphate

Spermine-modified Antheraea pernyi silk fibroin as a gene delivery carrier

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