Suppression of transforming growth factor β signaling promotes ground state pluripotency from single blastomeres

Hassani, Seyedeh‐Nafiseh; Pakzad, Mohammad; Asgari, Behrouz; Taei, Adeleh; Baharvand, Hossein

doi:10.1093/humrep/deu134

Cited by 18 publications

(30 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, we used different serum-free media supplemented with the R2i small molecule compound and cytokine LIF, which had previously shown the valuable effects on generation and long-term maintenance of the ESC and EGC lines from various mouse strains and rat. [13][14][15][16] Our analysis showed that N2B27/ R2i + LIF most effectively generated PSC lines from both stage X and HH28 chick embryos and supported further passaging. This result encouraged us to evaluate various small molecules where we determined that R2i was more efficient to derive chick PSCs compared to other evaluated chemical compounds ( Table 1 and Figure 2).…”

Section: Discussionmentioning

confidence: 82%

“…Further experiments were performed to design an optimal condition for in vitro derivation of ES‐like cells in the presence of a chemically defined medium. Here, we used different serum‐free media supplemented with the R2i small molecule compound and cytokine LIF, which had previously shown the valuable effects on generation and long‐term maintenance of the ESC and EGC lines from various mouse strains and rat . Our analysis showed that N2B27/R2i + LIF most effectively generated PSC lines from both stage X and HH28 chick embryos and supported further passaging.…”

Section: Discussionmentioning

confidence: 90%

See 1 more Smart Citation

Effects of various culture conditions on pluripotent stem cell derivation from chick embryos

Farzaneh

Zare

Hassani

et al. 2018

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

Pluripotent stem cell (PSC) lines derived from embryonated avian eggs are a convenient platform for production of various recombinant proteins and vaccines. In chicks, both embryonic stem cells (ESC) and embryonic germ cells (EGC) are considered to be pluripotent cells obtained from early blastodermal cells (stage X) and gonadal tissues (stage HH28), respectively. However, the establishment and long-term maintenance of avian PSC lines faces several challenges and differs in efficiency between chick strains. This study aims to determine the effects of PSC culture media, including serum-based and serum-free media as well as various feeder layers, growth factors, and small molecules on derivation and maintenance of avian embryonic derived-PSCs. Our results have shown that among the different culture conditions, N2B27 serum-free medium supplemented with PD0325901 and SB431542, MEK and TGFβ chemical inhibitors, named as R2i and cytokine leukemia inhibitory factor (LIF) improved PSC derivation from stages X- and HH28 embryos. The application of N2B27/R2i + LIF medium validates the effect of defined pluripotency supporting medium on efficient derivation of chick PSCs and facilitates the use of these cells in biotechnology and biobanking of valuable species.

show abstract

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 90%

Effects of various culture conditions on pluripotent stem cell derivation from chick embryos

Farzaneh

Zare

Hassani

et al. 2018

J of Cellular Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has previously been demonstrated that the augmented BMP4 signaling pathway plays a key role in R2i pluripotency, whereas it does not make a difference in the 2i condition ( Gomes Fernandes et al., 2016 , Hassani et al., 2014b ). Despite the high degree of similarity between the R2i and 2i conditions, as well as the strength of R2i in supporting pluripotency, i.e., in establishing and maintaining ESCs from single blastomeres ( Hassani et al., 2014a ) and embryonic germ cells from the PGCs of mice and rats ( Attari et al., 2014 , Mohammadi et al., 2015 ), these two conditions work in different ways.…”

Section: Discussionmentioning

confidence: 99%

“…We showed that in the serum-free condition, R2i could support the ground state of pluripotency. Also, as well as from blastocysts, R2i could support the highly efficient ESC derivation from a wider developmental window, from single blastomeres of two to eight cell-stage mouse embryos with an efficiency of more than 2-fold higher than the 2i condition ( Hassani et al., 2014a ). Moreover, when cells underwent long-term passaging, they exhibited more genomic stability with R2i support compared with 2i ( Hassani et al., 2014b ).…”

Section: Introductionmentioning

confidence: 99%

Blockage of the Epithelial-to-Mesenchymal Transition Is Required for Embryonic Stem Cell Derivation

et al. 2017

Self Cite

View full text Add to dashboard Cite

SummaryPluripotent cells emanate from the inner cell mass (ICM) of the blastocyst and when cultivated under optimal conditions immortalize as embryonic stem cells (ESCs). The fundamental mechanism underlying ESC derivation has, however, remained elusive. Recently, we have devised a highly efficient approach for establishing ESCs, through inhibition of the MEK and TGF-β pathways. This regimen provides a platform for dissecting the molecular mechanism of ESC derivation. Via temporal gene expression analysis, we reveal key genes involved in the ICM to ESC transition. We found that DNA methyltransferases play a pivotal role in efficient ESC generation. We further observed a tight correlation between ESCs and preimplantation epiblast cell-related genes and noticed that fundamental events such as epithelial-to-mesenchymal transition blockage play a key role in launching the ESC self-renewal program. Our study provides a time course transcriptional resource highlighting the dynamics of the gene regulatory network during the ICM to ESC transition.

show abstract

“…Optimizing the culture conditions and inhibiting the TGF-β pathway enabled the efficient isolation of ESCs from individual cells of embryos at the two-to eight-cell stage; however, these cells were demonstrated to contribute only to the embryonic germ layers in vivo (Hassani et al 2014). More recently, it was shown that by inhibiting MAPKs, SRC and Hippo signaling pathways, and poly-ADP-ribosylation regulators, ESCs with an expanded developmental potential (EPSCs) could be generated (Yang et al 2017).…”

Section: Mouse Pluripotent Stem Cellsmentioning

confidence: 99%

Specification of the First Mammalian Cell Lineages In Vivo and In Vitro

White

Plachta

2019

Cold Spring Harb Perspect Biol

View full text Add to dashboard Cite

Our understanding of how the first mammalian cell lineages arise has been shaped largely by studies of the preimplantation mouse embryo. Painstaking work over many decades has begun to reveal how a single totipotent cell is transformed into a multilayered structure representing the foundations of the body plan. Here, we review how the first lineage decision is initiated by epigenetic regulation but consolidated by the integration of morphological features and transcription factor activity. The establishment of pluripotent and multipotent stem cell lines has enabled deeper analysis of molecular and epigenetic regulation of cell fate decisions. The capability to assemble these stem cells into artificial embryos is an exciting new avenue of research that offers a long-awaited window into cell fate specification in the human embryo. Together, these approaches are poised to profoundly increase our understanding of how the first lineage decisions are made during mammalian embryonic development.

show abstract

Suppression of transforming growth factor β signaling promotes ground state pluripotency from single blastomeres

Abstract: This study was funded by grants provided from Royan Institute, the Iranian Council of Stem Cell Research and Technology and the Iran National Science Foundation. The authors have no conﬂict of interest to declare.

Cited by 18 publications

References 21 publications

Effects of various culture conditions on pluripotent stem cell derivation from chick embryos

Effects of various culture conditions on pluripotent stem cell derivation from chick embryos

Blockage of the Epithelial-to-Mesenchymal Transition Is Required for Embryonic Stem Cell Derivation

Specification of the First Mammalian Cell Lineages In Vivo and In Vitro

Contact Info

Product

Resources

About