Suppression of the non-linear background in a multimode fibre CARS endoscope

Pikálek, Tomáš; Stibůrek, Miroslav; Simpson, Stephen H.; Čižmár, Tomáš; Trägårdh, Johanna

doi:10.1364/boe.450375

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…MMF endoscopes have further been demonstrated to offer even non-linear chemical imaging modalities, particularly CARS [29]. The most advanced version [187] utilizes femtosecond laser pulses with spectral focusing modality [188] and composite fiber probes for suppression of strong background signals originating in non-linear processes inside the fiber. Importantly, this concept enabled reduction of the pixel dwell time to millisecond intervals (see Fig.…”

Section: Chemical Contrast Imagingmentioning

confidence: 99%

Controlling light propagation in multimode fibers for imaging, spectroscopy, and beyond

Cao¹,

Čižmár²,

Turtaev³

et al. 2023

Adv. Opt. Photon.

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Light transport in a highly multimode fiber exhibits complex behavior in space, time, frequency and polarization, especially in the presence of mode coupling. The newly developed techniques of spatial wavefront shaping turn out to be highly suitable to harness such enormous complexity: a spatial light modulator enables precise characterization of field propagation through a multimode fiber, and by adjusting the incident wavefront it can accurately tailor the transmitted spatial pattern, temporal profile and polarization state. This unprecedented control leads to multimode fiber applications in imaging, endoscopy, optical trapping and microfabrication. Furthermore, the output speckle pattern from a multimode fiber encodes spatial, temporal, spectral and polarization properties of the input light, allowing such information to be retrieved from spatial measurements only. This article provides an overview of recent advances and breakthroughs in controlling light propagation in multimode fibers, and discusses newly emerging applications.

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Section: Chemical Contrast Imagingmentioning

confidence: 99%

Controlling light propagation in multimode fibers for imaging, spectroscopy, and beyond

Cao¹,

Čižmár²,

Turtaev³

et al. 2023

Adv. Opt. Photon.

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“…Coherent anti-Stokes Raman scattering (CARS) is an effective approach to enhance the Raman signal and thereby significantly reduce the signal acquisition time 145 . In 2019, Trägårdh et al successfully implemented the CARS imaging through the MFSE and achieved ~1 ms integration time for Raman signal acquisition in the epi-detection condition, which is several orders faster than the spontaneous Raman imaging 146 . In the experiment, two synchronized pump and Stokes pulses, after wavefront shaping by the same SLM, were focused onto the sample using the MMF.…”

Section: Raman Imagingmentioning

confidence: 99%

Optical scanning endoscope via a single multimode optical fiber

Wu,

Zhu,

et al. 2024

OES

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Optical endoscopy has become an essential diagnostic and therapeutic approach in modern biomedicine for directly observing organs and tissues deep inside the human body, enabling non-invasive, rapid diagnosis and treatment. Optical fiber endoscopy is highly competitive among various endoscopic imaging techniques due to its high flexibility, compact structure, excellent resolution, and resistance to electromagnetic interference. Over the past decade, endoscopes based on a single multimode optical fiber (MMF) have attracted widespread research interest due to their potential to significantly reduce the footprint of optical fiber endoscopes and enhance imaging capabilities. In comparison with other imaging principles of MMF endoscopes, the scanning imaging method based on the wavefront shaping technique is highly developed and provides benefits including excellent imaging contrast, broad applicability to complex imaging scenarios, and good compatibility with various well-established scanning imaging modalities. In this review, various technical routes to achieve light focusing through MMF and procedures to conduct the scanning imaging of MMF endoscopes are introduced. The advancements in imaging performance enhancements, integrations of various imaging modalities with MMF scanning endoscopes, and applications are summarized. Challenges specific to this endoscopic imaging technology are analyzed, and potential remedies and avenues for future developments are discussed.

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“…Typically, these devices offer a field of view of up to 450 μm and a lateral resolution of approximately 1 μm. Currently, the research focuses on improving imaging quality (Pikálek et al 2022 ).…”

Section: Techniques and Instrumentation For Label-free Imaging And An...mentioning

confidence: 99%

Vibrational spectroscopy and multiphoton microscopy for label-free visualization of nervous system degeneration and regeneration

Galli,

Uckermann

2023

Biophys Rev

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Neurological disorders, including spinal cord injury, peripheral nerve injury, traumatic brain injury, and neurodegenerative diseases, pose significant challenges in terms of diagnosis, treatment, and understanding the underlying pathophysiological processes. Label-free multiphoton microscopy techniques, such as coherent Raman scattering, two-photon excited autofluorescence, and second and third harmonic generation microscopy, have emerged as powerful tools for visualizing nervous tissue with high resolution and without the need for exogenous labels. Coherent Raman scattering processes as well as third harmonic generation enable label-free visualization of myelin sheaths, while their combination with two-photon excited autofluorescence and second harmonic generation allows for a more comprehensive tissue visualization. They have shown promise in assessing the efficacy of therapeutic interventions and may have future applications in clinical diagnostics. In addition to multiphoton microscopy, vibrational spectroscopy methods such as infrared and Raman spectroscopy offer insights into the molecular signatures of injured nervous tissues and hold potential as diagnostic markers. This review summarizes the application of these label-free optical techniques in preclinical models and illustrates their potential in the diagnosis and treatment of neurological disorders with a special focus on injury, degeneration, and regeneration. Furthermore, it addresses current advancements and challenges for bridging the gap between research findings and their practical applications in a clinical setting.

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Suppression of the non-linear background in a multimode fibre CARS endoscope

Cited by 5 publications

References 38 publications

Controlling light propagation in multimode fibers for imaging, spectroscopy, and beyond

Controlling light propagation in multimode fibers for imaging, spectroscopy, and beyond

Optical scanning endoscope via a single multimode optical fiber

Vibrational spectroscopy and multiphoton microscopy for label-free visualization of nervous system degeneration and regeneration

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