Suppression of the ER-Localized AAA ATPase NgCDC48 Inhibits Tobacco Growth and Development

Bae, Hyun Cheol; Choi, Soo Min; Yang, Seong Wook; Pai, Hyun Sook; Kim, Woo Taek

doi:10.1007/s10059-009-0101-4

Cited by 21 publications

(18 citation statements)

References 41 publications

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“…Total chlorophyll (chlorophyll a + chlorophyll b) was extracted from the drought-treated leaves as described previously by Bae et al (2009). The amount of Pro in normal and drought-treated leaves was determined as described by Claussen (2005).…”

Section: Drought Stress Analysismentioning

confidence: 99%

Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses

et al. 2016

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Lipid droplets (LDs) act as repositories for fatty acids and sterols, which are used for various cellular processes such as energy production and membrane and hormone synthesis. LD-associated proteins play important roles in seed development and germination, but their functions in postgermination growth are not well understood. Arabidopsis (Arabidopsis thaliana) contains three SRP homologs (SRP1, SRP2, and SRP3) that share sequence identities with small rubber particle proteins of the rubber tree (Hevea brasiliensis). In this report, the possible cellular roles of SRPs in postgermination growth and the drought tolerance response were investigated. Arabidopsis SRPs appeared to be LD-associated proteins and displayed polymerization properties in vivo and in vitro. SRP-overexpressing transgenic Arabidopsis plants (35S:SRP1, 35S:SRP2, and 35S:SRP3) exhibited higher vegetative and reproductive growth and markedly better tolerance to drought stress than wild-type Arabidopsis. In addition, constitutive over-expression of SRPs resulted in increased numbers of large LDs in postgermination seedlings. In contrast, single (srp1, 35S:SRP2-RNAi, and srp3) and triple (35S:SRP2-RNAi/srp1srp3) loss-of-function mutant lines exhibited the opposite phenotypes. Our results suggest that Arabidopsis SRPs play dual roles as positive factors in postgermination growth and the drought stress tolerance response. The possible relationships between LD-associated proteins and the drought stress response are discussed.Environmental stresses, including drought, high salinity, oxidative stress, and unfavorable temperatures, profoundly affect the growth and development of higher plants. Because of their sessile life cycle, plants have developed self-protective mechanisms to increase their tolerance to short-and long-term stresses by triggering diverse sets of signal transduction pathways and activating stress-responsive genes. The genetic and cellular mechanisms in response to abiotic stress have been widely documented in higher plants (Shinozaki and

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Section: Drought Stress Analysismentioning

confidence: 99%

Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses

et al. 2016

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“…Chlorophylls were extracted and analyzed from drought stress-treated wild-type and CaSRP1-overexpressing leaves using 80% acetone as described previously (Bae et al 2009). The remaining leaf extracts were dried at 105°C for 16 h and weights of the dried extracts were measured (Welti et al 2002).…”

Section: Chlorophyll Measurementmentioning

confidence: 99%

Constitutive expression of CaSRP1, a hot pepper small rubber particle protein homolog, resulted in fast growth and improved drought tolerance in transgenic Arabidopsis plants

Kim

Seo

Lee

et al. 2010

Planta

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Transient and long-term shortages of fresh water are major adverse environmental factors that cause dramatic reductions in crop production and distribution globally. In this study, we isolated a full-length CaSRP1 (Capsicum annuum stress-related protein 1) cDNA, which was rapidly induced by dehydration in hot pepper plants. The predicted CaSRP1 protein sequence exhibited significant amino acid identity to putative stress-related proteins and the small rubber particle protein (SRPP) found in rubber trees (Hevea brasiliensis). To study the cellular functions of CaSRP1, transgenic Arabidopsis plants (35S:CaSRP1) that constitutively expressed the CaSRP1 gene were constructed. Overexpression of CaSRP1 resulted in enhanced root and shoot growth and earlier bolting in the transgenic plants relative to wild-type plants. In addition, 35S:CaSRP1 overexpressors exhibited enhanced tolerance to drought stress as compared to the control plants. These results suggest that CaSRP1 plays dual functions as a positive factor for tissue growth and development and for drought-defensive responses. A possible cellular function of SRPP homologs in non-rubber-producing plants in relation to drought stress tolerance is discussed.

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“…The function of the CDC48A–SERK1 interaction is unclear. Considering the roles of animal CDC48A in ER and Golgi membrane fusion, and vesicle trafficking (Bae et al, 2009) CDC48A–SERK1 interaction with SERK1 was predicted to assist in receptor maturation.…”

Section: Endoplasmic Reticulum – a Hub For Receptor Biogenesis And Mamentioning

confidence: 99%

A Model for the Biosynthesis and Transport of Plasma Membrane-Associated Signaling Receptors to the Cell Surface

Popescu

2012

Front. Plant Sci.

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Intracellular protein transport is emerging as critical in determining the outcome of receptor-activated signal transduction pathways. In plants, relatively little is known about the nature of the molecular components and mechanisms involved in coordinating receptor synthesis and transport to the cell surface. Recent advances in this field indicate that signaling pathways and intracellular transport machinery converge and coordinate to render receptors competent for signaling at their plasma membrane (PM) activity sites. The biogenesis and transport to the cell surface of signaling receptors appears to require both general trafficking and receptor-specific factors. Several molecular determinants, residing or associated with compartments of the secretory pathway and known to influence aspects in receptor biogenesis, are discussed and integrated into a predictive cooperative model for the functional expression of signaling receptors at the PM.

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Suppression of the ER-Localized AAA ATPase NgCDC48 Inhibits Tobacco Growth and Development

Cited by 21 publications

References 41 publications

Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses

Arabidopsis Small Rubber Particle Protein Homolog SRPs Play Dual Roles as Positive Factors for Tissue Growth and Development and in Drought Stress Responses

Constitutive expression of CaSRP1, a hot pepper small rubber particle protein homolog, resulted in fast growth and improved drought tolerance in transgenic Arabidopsis plants

A Model for the Biosynthesis and Transport of Plasma Membrane-Associated Signaling Receptors to the Cell Surface

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