Suppression of STIM1 inhibits the migration and invasion of human prostate cancer cells and is associated with PI3K/Akt signaling inactivation

Zhou, Yibin; Gu, Peng; Li, Jian; Li, Feng; Zhu, Jin; Gao, Peng; Zang, Yu‐Feng; Wang, Yongchang; Shan, Yuxi; Yang, Dian

doi:10.3892/or.2017.5961

Cited by 34 publications

(26 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, similar to BC, PRLR/ Jak2/STAT5 is also the main signaling pathway for activation in prostate gland, and PRLR-triggered pro-tumorigenic pathways in PCa include PI3K/AKT [172]. In addition, AEP, SCL/TAL1, SIRT3, Snail, MED15, STIM1, ST6Gal-I, Glyoxalase 2, ASF1B, GPCR48/LGR4, AP4, GCN5, SAG/ RBX2 E3, miR-7, -101, -129, -133a-3p, and -4638-5p, as well as LncRNA HCG11 and ATB govern tumorigenesis, progression, metastasis, EMT or castration resistant of PCa cells via PI3K/AKT pathway [237][238][239][240][241][242][243][244][245][246][247][248][249][250][251][252][253][254][255][256]. Preclinical trial of dual BRD4/PI3K inhibitor SF2523 [257] as well as a few of clinical trials of PI3K/AKT inhibitors may develop new therapeutic strategies for PCa patients (Tables 2 and 3).…”

Section: Dysregulation Of the Pi3k/akt Pathway In The Genitourinary Smentioning

confidence: 99%

Role of PI3K/AKT pathway in cancer: the framework of malignant behavior

et al. 2020

View full text Add to dashboard Cite

Given that the PI3K/AKT pathway has manifested its compelling influence on multiple cellular process, we further review the roles of hyperactivation of PI3K/AKT pathway in various human cancers. We state the abnormalities of PI3K/AKT pathway in different cancers, which are closely related with tumorigenesis, proliferation, growth, apoptosis, invasion, metastasis, epithelial-mesenchymal transition, stem-like phenotype, immune microenvironment and drug resistance of cancer cells. In addition, we investigated the current clinical trials of inhibitors against PI3K/AKT pathway in cancers and found that the clinical efficacy of these inhibitors as monotherapy has so far been limited despite of the promising preclinical activity, which means combinations of targeted therapy may achieve better efficacies in cancers. In short, we hope to feature PI3K/ AKT pathway in cancers to the clinic and bring the new promising to patients for targeted therapies.

show abstract

Section: Dysregulation Of the Pi3k/akt Pathway In The Genitourinary Smentioning

confidence: 99%

Role of PI3K/AKT pathway in cancer: the framework of malignant behavior

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Furthermore, knockdown of STIM1 expression accelerated motility of melanoma cells, indicating that STIM1 may be an anti-metastasis gene [16]. However, more recent evidence suggests that STIM/Orai signaling accelerates migration and cell cycle progression in a number of human cancers, including breast [17], prostate [18], gastric [19] and colorectal cancer [20]. Recently, Wang et al [21] reported that the expression of STIM1 was significantly increased in lung cancer tissues compared with that in non-neoplastic lung tissues.…”

Section: Introductionmentioning

confidence: 99%

Knockdown of STIM1 expression inhibits non-small-cell lung cancer cell proliferation in vitro and in nude mouse xenografts

Zeng

et al. 2019

Bioengineered

View full text Add to dashboard Cite

Stromal interaction molecule 1 (STIM1) is a calcium-sensing protein localized in the membrane of the endoplasmic reticulum. The expression of STIM1 has been shown to be closely associated with cell proliferation. The aim of the present study was to investigate the role of STIM1 in the regulation of cancer progression and its clinical relevance. The data demonstrated that the expression of the STIM1 was significantly higher in non-small-cell lung cancer (NSCLC) tissues than in benign lesions and was associated with advanced NSCLC T stage. Knockdown of STIM1 expression in NSCLC cell lines A549 and SK-MES-1 significantly inhibited cell proliferation and induces A549 and SK-MES-1 cell arrest at the G2/M and S phases of the cell cycle. Western blotting showed that the expression of cyclin-dependent kinase (CDK) 1 and CDK2 were reduced while knockdown of STIM1 expression. Furthermore, knockdown of STIM1 in NSCLC cells significantly reduced the levels of xenograft tumor growth in nude mice. These data indicate that aberrant expression of the STIM1 protein may contribute to NSCLC progression. Future studies should focus on targeting STIM1 as a novel strategy for NSCLC therapy.

show abstract

“…28 PI3K phosphorylates AKT and consequently facilitates tumourigenesis and cancer progression through its downstream targets. 29 In our study, Ataxia-telangiectasia mutated kinase (ATM) was found to be highly up-regulated in MTX-resistant xenografts, and observed as a hub gene in the network. This gene is also highly expressed in non-small cell lung cancer (NSCL) exposed to carbon ion irradiation.…”

Section: Discussionmentioning

confidence: 65%

Transcriptome analysis of differentially expressed genes and pathways associated with mitoxantrone treatment prostate cancer

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

The global physiological function of specifically expressed genes of mitoxantrone (MTX)‐resistant prostate cancer (PCa) is unclear. In this study, gene expression pattern from microarray data was investigated for identifying differentially expressed genes (DEGs) in MTX‐resistant PCa xenografts. Human PCa cell lines DU145 and PC3 were cultured in vitro and xenografted into severe combined immunodeficiency (SCID) mice, treated with MTX intragastrically, three times a week until all mice relapsed. Gene expression profiles of the xenografts from castrated mice were performed with Affymetrix human whole genomic oligonucleotide microarray. The Cytoscape software was used to investigate the relationship between proteins and the signalling transduction network. A total of 355 overlapping genes were differentially expressed in MTX‐resistant DU145R and PC3R xenografts. Of these, 16 genes were selected to be validated by quantitative real‐time PCR (qRT‐PCR) in these xenografts, and further tested in a set of formalin‐fixed, paraffin‐embedded and optimal cutting temperature (OCT) clinical tumour samples. Functional and pathway enrichment analyses revealed that these DEGs were closely related to cellular activity, androgen synthesis, DNA damage and repair, also involved in the ERK/MAPK, PI3K/serine‐threonine protein kinase, also known as protein kinase B, PKB (AKT) and apoptosis signalling pathways. This exploratory analysis provides information about potential candidate genes and may bring new insights into the molecular cascade involvement in MTX‐resistant PCa.

show abstract

Suppression of STIM1 inhibits the migration and invasion of human prostate cancer cells and is associated with PI3K/Akt signaling inactivation

Cited by 34 publications

References 44 publications

Role of PI3K/AKT pathway in cancer: the framework of malignant behavior

Role of PI3K/AKT pathway in cancer: the framework of malignant behavior

Knockdown of STIM1 expression inhibits non-small-cell lung cancer cell proliferation in vitro and in nude mouse xenografts

Transcriptome analysis of differentially expressed genes and pathways associated with mitoxantrone treatment prostate cancer

Contact Info

Product

Resources

About