Suppression of spinach wilt disease by biological soil disinfestation incorporated with Brassica juncea plants in association with changes in soil bacterial communities

Mowlick, Subrata; Yasukawa, H.; Inoue, Takashi; Takehara, Toshiaki; Kaku, Nobuo; Ueki, Katsuji; Ueki, Atsuko

doi:10.1016/j.cropro.2013.08.012

Cited by 52 publications

(41 citation statements)

References 32 publications

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“…Disease suppression by the brassicas could also be explained by indirect effects, and multiple mechanisms of action, other than activity of GSL hydrolysis products, have been suggested. Since Brassicaceae amendments have been shown to alter soil microbial communities several authors have proposed possible disease suppressive effects (Omirou et al 2011;Wang et al 2014;Mowlick et al 2013). As in the present study, Rumberger and Marshner (2004) showed ITC release from living roots of canola and suggested that root pathogens may be directly or indirectly negatively affected by ITCs via observed changes in rhizosphere microbial community composition.…”

Section: Discussionsupporting

confidence: 67%

Brassicaceae cover crops reduce Aphanomyces pea root rot without suppressing genetic potential of microbial nitrogen cycling

et al. 2015

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Aims Brassicaceae cover crops can be used to suppress soil-borne pathogens. The aim was to investigate the effect of different brassicas with different glucosinolate profiles on the development of Aphanomyces pea root rot in subsequent pea plants, and the genetic potential of free-living N 2 -fixing bacteria and ammonia oxidising bacteria (AOB) and archaea (AOA) performing key soil ecosystem services. Methods The Brassicaceae species Brassica juncea and Sinapis alba and non-Brassicaceae species Secale cereale were grown for 11-weeks in Aphanomyces euteiches infested soil at low and high nitrogen (N) fertiliser doses. After removing both shoots and roots of the cover crops, peas were grown as a bioassay to evaluate Aphanomyces pea root rot development. Soil was sampled before harvesting the cover crops and at the end of the bioassay. Volatile compounds were collected in the root-soil environment before harvesting the Brassicaceae cover crops to determine the concentration of isothiocyanates. The abundance of genes involved in N 2 -fixing bacteria and ammonia oxidation in AOA and AOB were assessed. Results Pea root rot disease severity was reduced in Brassicaceae grown soil at the high N fertiliser dose. This was associated with increased growth of the cover crops. The growth of Brassicaceae did not suppress the abundance of N-cycling microbial communities, but rather increased the AOB at the end of the bioassay, most likely due to increased N availability. The disease suppressive effect was higher with S. alba than with B. juncea, and this coincided with a more diverse composition and higher concentration of aliphatic ITCs released from S. alba roots. Fewer nodules were formed after the Brassicaceae crops, especially Sinapis alba. Conclusions Brassicaceae cover crops, particularly S. alba, can be used to control soil-borne pathogens without major side effects on the genetic potential of beneficial soil microorganisms involved in N cycling. However, less nodule formation after brassicas indicates an effect on rhizobium activity.

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Section: Discussionsupporting

confidence: 67%

Brassicaceae cover crops reduce Aphanomyces pea root rot without suppressing genetic potential of microbial nitrogen cycling

et al. 2015

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“…The second and third treatments as well as cropping of spinach were carried out in parallel with the field experiment (Exp. 2) described previously (Mowlick et al, 2013c).…”

Section: A Bsd Experiments In a Field Greenhouse Using Radish Residuementioning

confidence: 99%

“…The amount of water for irrigation (the ratio of soil and water, 4.3:1.2) was determined based on our previous BSD field experiments (Mowlick et al, 2013c).…”

Section: A Model Experiments Of Bsd Using Pots and Plastic Bagsmentioning

confidence: 99%

“…The concentrations of volatile fatty acids (VFAs) in the soil samples collected were measured by gas chromatography (Hitachi G-3900) as described previously (Mowlick et al, 2012;2013c).…”

Section: Determination Of Volatile Fatty Acids (Vfas) Concentrations mentioning

confidence: 99%

“…spinaciae (wilt pathogen of spinach). Furthermore, B. juncea plants were effectively used in field experiments as BSD material to combat spinach wilt (Mowlick et al, 2013c). Using molecular analyses such as polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and clone library methods based on 16S rRNA gene sequences, we observed that bacterial communities in these BSD-treated soil samples were greatly changed and anaerobic groups, especially in the class Clostridia of the phylum Firmicutes, became major bacterial groups in the communities together with some other aerobic or facultatively anaerobic bacteria in the classes Bacilli and Gammaproteobacteria.…”

Section: Introductionmentioning

confidence: 99%

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