Suppression of red rot disease by Bacillus sp. based biopesticide formulated in non-sterilized sugarcane filter cake

Hassan, Muhammad Nadeem; Namood-e-Sahar,; Shah, Syed Zia-Ul-Husnain; Afghan, Shahid; Hafeez, Fauzia Yusuf

doi:10.1007/s10526-015-9673-4

Cited by 23 publications

(11 citation statements)

References 25 publications

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“…Liu et al (2010) demonstrated better control of S. sclerotiorum by cell-free filtrate of endophytic bacteria Yc8 application on the rapeseed leaves 24 h before fungal inoculation. Under field conditions, Bacillus isolate has a potential to be used as biobacteriocide in tomato plants (Santiago et al 2015) and for the control of red rot in sugarcane (Hassan et al 2015). However, the presence of biologically active metabolite produced by the antagonist, which diffused in the agar media, can be indicated by the zone of inhibition seen in the dual culture plates.…”

Section: Discussionmentioning

confidence: 99%

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

2017

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A study was executed in a direction to attenuate Sclerotinia stalk rot (SSR) disease through biocontrol agent and also to enhance crop productivity. Culture filtrate of bacterial strain YSPMK11 inhibited growth of Sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. Interaction studies revealed maximum (81.50%) growth inhibition at 35°C and pH 7.0 after 72 h incubation period with 15% culture filtrate. Based upon 16S rRNA gene sequence strain, YSPMK11 was identified as Bacillus pumilus. Furthermore, the genome of this isolate was searched for antimicrobial lipopeptide, i.e., ItuD and SrfC genes. The PCR amplification results showed the presence of both these lipopeptide genes in isolate YSPMK11. Iturin A as antifungal compound was identified as major components of fraction through GC/MS. In field experiments, the application of strain YSPMK11 cell suspension (10 8 CFU/ml) suppressed disease severity by 93% and increased curd yield by 36% which was more that of commercially used fungicide in farmer practices under mid-hills of Himachal Pradesh.Conclusively, our study is first to demonstrate the effect of B. pumilus strain YSPMK11 in suppression of SSR under field conditions and would be employed as an efficient biocontrol agent to replace commercial fungicides in cauliflower cropping system. In addition, the presence of both lipopeptide genes (ItuD and SrfC) and iturin A in this isolate makes him potent strain for biological control application in agriculture.

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Section: Discussionmentioning

confidence: 99%

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

2017

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“…The homogenate was centrifuged at 10,000 rpm, 4°C for 15 min and supernatant was used as crude enzyme extract [ 50 ]. Absorbance of PO, PPO and PAL, was observed on spectrophotometer at 280 nm, 450nm, 460 nm respectively [ 8 , 38 , 51 – 54 ]. Enzyme activity was expressed as change in absorbance min/ g fresh weight (FW).…”

Section: Methodsmentioning

confidence: 99%

“…The Bacillus spp. were isolated by serial dilution as described in our previous studies [ 37 , 38 , 40 ].…”

Section: Methodsmentioning

confidence: 99%

Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae

et al. 2017

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Plant growth promoting rhizobacteria (PGPR) are found to control the plant diseases by adopting various mechanisms. Induced systemic resistance (ISR) is an important defensive strategy manifested by plants against numerous pathogens especially infecting at aerial parts. Rhizobacteria elicit ISR by inducing different pathways in plants through production of various metabolites. In the present study, potential of Bacillus spp. KFP-5, KFP-7, KFP-17 was assessed to induce antioxidant enzymes against Pyricularia oryzae infection in rice. The antagonistic Bacillus spp. significantly induced antioxidant defense enzymes i-e superoxide dismutase (1.7–1.9-fold), peroxidase (3.5–4.1-fold), polyphenol oxidase (3.0–3.8-fold), phenylalanine ammonia-lyase (3.9–4.4-fold), in rice leaves and roots under hydroponic and soil conditions respectively. Furthermore, the antagonistic Bacillus spp significantly colonized the rice plants (2.0E+00–9.1E+08) and secreted multiple biocontrol determinants like protease (1.1–5.5 U/mg of soil or U/mL of hydroponic solution), glucanase, (1.0–1.3 U/mg of soil or U/mL of hydroponic solution), siderophores (6.5–42.8 μg/mL or mg) in the rhizosphere of different rice varieties. The results showed that treatment with Bacillus spp. enhanced the antioxidant defense activities in infected rice, thus alleviating P. oryzae induced oxidative damage and suppressing blast disease incidence.

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“…However, some species belong to the same genus as our identified species were confirmed to have positive effect on plant growth. For instance, some species of Bacillus, Pseudomonas, Acremonium and Humicola could promote plant growth due to their antagonistic activity, the production of IAA or specific substance to activate resistance for control of diseases (Dharni et al 2014;Hassan et al 2015;Khabbaz et al 2015;Wenhsiung 2009;Yang et al 2014). On the other side, some species of these genera showed negative effect on plant growth due to their broadspectrum pathogenic activity (Elbanna et al 2014;Furuya et al 2005;Iakovleva et al 2013;Racedo et al 2013;Zhang et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Assaying the potential autotoxins and microbial community associated with Rehmannia glutinosa replant problems based on its ‘autotoxic circle’

Bao

Wang

et al. 2016

Plant Soil

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Aims This study attempted to elucidate the underlying correlations among replant problems of Rehmannia glutinosa, the autotoxins and microbes within its rootzone soil. Methods Different root-zone soils of R. glutinosa were collected to identify the potential autotoxins, to quantify the phenolic acids' concentration and to analyse the microbial community composition. Bioassay was conducted to assess the autotoxic potential of these samples and candidate autotoxins. Results R. glutinosa autotoxicity was presented within a 20 cm radius of the plant. Concentrations of the phenolic acids were significantly higher within this range. Twenty compounds and 17 microbes emerged in this range after the R. glutinosa cultivation. Further seedling test showed that four selected compounds possessed conspicuous autotoxic activity and a synergistic effect was observed. Moreover, one potential beneficial fungi (99 % similar with Arthrobotrys oligospora) and one potential pathogens (100 % similar with Acremonium sclerotigenum) were identified. Conclusions Our work defined the 'autotoxic circle' of R. glutinosa and identified the corresponding autotoxins and microbes. We suggest that R. glutinosa has various autotoxins and that these autotoxins and specific microbes deserve further investigation to unravel their interaction with R. glutinosa in replant problems' occurrence.

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Suppression of red rot disease by Bacillus sp. based biopesticide formulated in non-sterilized sugarcane filter cake

Cited by 23 publications

References 25 publications

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum

Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae

Assaying the potential autotoxins and microbial community associated with Rehmannia glutinosa replant problems based on its ‘autotoxic circle’

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