Suppression of red cell diffusional water permeability by lipophilic solutes

Lahajnar, G.; Maček, Peter; Zupančić, I.

doi:10.1016/s0302-4598(00)00100-8

Cited by 10 publications

(4 citation statements)

References 42 publications

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“…Even alcohols with P d slightly lower than P d water (e.g., methanol and ethanol), have been shown to cause no net water movement across the RBC membrane [49]. Recently, it has been shown that methanol, ethanol, propanol and butanol may even suppress water diffusion across RBC membrane [50]. The phenomenon was not related to osmolarity changes, but to the dual effects of alcohols on RBC membrane components: water channels have decreased permeability and the lipid bilayer has an increased permeability for water, with the later being related to increased membrane fluidity [51].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size

et al. 2013

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The role of membrane fluidity in determining red blood cell (RBC) deformability has been suggested by a number of studies. The present investigation evaluated alterations of RBC membrane fluidity, deformability and stability in the presence of four linear alcohols (methanol, ethanol, propanol and butanol) using ektacytometry and electron paramagnetic resonance (EPR) spectroscopy. All alcohols had a biphasic effect on deformability such that it increased then decreased with increasing concentration; the critical concentration for reversal was an inverse function of molecular size. EPR results showed biphasic changes of near-surface fluidity (i.e., increase then decrease) and a decreased fluidity of the lipid core; rank order of effectiveness was butanol > propanol > ethanol > methanol, with a significant correlation between near-surface fluidity and deformability (r = 0.697; p<0.01). The presence of alcohol enhanced the impairment of RBC deformability caused by subjecting cells to 100 Pa shear stress for 300 s, with significant differences from control being observed at higher concentrations of all four alcohols. The level of hemolysis was dependent on molecular size and concentration, whereas echinocytic shape transformation (i.e., biconcave disc to crenated morphology) was observed only for ethanol and propanol. These results are in accordance with available data obtained on model membranes. They document the presence of mechanical links between RBC deformability and near-surface membrane fluidity, chain length-dependence of the ability of alcohols to alter RBC mechanical behavior, and the biphasic response of RBC deformability and near-surface membrane fluidity to increasing alcohol concentrations.

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Section: Discussionmentioning

confidence: 99%

The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size

et al. 2013

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“…However, lipophilic solutes such as ethanol have a known capacity to suppress the permeability to diffusional water in red blood cells due to the net effect of two opposing actions: the reduction of channel-mediated permeability, associated with the denaturation of aquaporins, and the increase in lipid-mediated permeability, probably associated with the formation of aqueous leaks in the membrane hydrophobic barrier [33]. An increase in osmotic pressure will exacerbate the removal of water by this second action of ethanol.…”

Section: Effects Of Ethanol On Human Erythrocytesmentioning

confidence: 99%

“…The composition and physicochemical properties of the external medium also affect the conformation [20,28,31] and properties [32][33][34][35] of the erythrocyte membrane. Glycerol converts biconcave erythrocytes into cells with membrane internalizations (stomatocytes).…”

Section: Introductionmentioning

confidence: 99%

Effects of glycerol and sorbitol on the thermal dependence of the lysis of human erythrocytes by ethanol

Penha-Silva

Arvelos

Cunha

et al. 2008

Bioelectrochemistry

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“…Transport through membranes is one of the most fundamental functionalities of the bilayers [16,17,18,19]. While transport channels or transport proteins are responsible for the transport of larger molecules, small molecules, such as water, can pass the bilayer with a certain probability.…”

Section: Introductionmentioning

confidence: 99%

Collective molecular dynamics in proteins and membranes (Review)

Rheinstädter

2008

Biointerphases

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The understanding of dynamics and functioning of biological membranes and, in particular, of membrane embedded proteins is one of the most fundamental problems and challenges in modern biology and biophysics. In particular, the impact of membrane composition and properties and of structure and dynamics of the surrounding hydration water on protein function is an upcoming topic, which can be addressed by modern experimental and computational techniques. Correlated molecular motions might play a crucial role for the understanding of, for instance, transport processes and elastic properties, and might be relevant for protein function. Experimentally that involves determining dispersion relations for the different molecular components, i.e., the length scale dependent excitation frequencies and relaxation rates. Only very few experimental techniques can access dynamical properties in biological materials on the nanometer scale, and resolve dynamics of lipid molecules, hydration water molecules, and proteins and the interaction between them. In this context, inelastic neutron scattering turned out to be a very powerful tool to study dynamics and interactions in biomolecular materials up to relevant nanosecond time scales and down to the nanometer length scale. The author reviews and discusses inelastic neutron scattering experiments to study membrane elasticity and protein-protein interactions of membrane embedded proteins.

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Suppression of red cell diffusional water permeability by lipophilic solutes

Cited by 10 publications

References 42 publications

The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size

The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size

Effects of glycerol and sorbitol on the thermal dependence of the lysis of human erythrocytes by ethanol

Collective molecular dynamics in proteins and membranes (Review)

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