Suppression of progesterone production by stresscopin/urocortin 3 in cultured human granulosa-lutein cells

Yata, Ai; Nakabayashi, Koji; Wakahashi, Senn; Maruo, Nobuyuki; Ohara, Noriyuki; Maruo, Takeshi

doi:10.1093/humrep/dep063

Cited by 7 publications

(4 citation statements)

References 33 publications

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“…In mammals, CRH and urocortins have important roles in steroidogenesis and fertility (Florio et al, 2004;Murase et al, 2002). In cultured ovarian cells, CRH inhibits steroidogenesis (Calogero et al, 1996), as does Ucn3 (Yata et al, 2009). While the functions of urocortins in teleost gonads remain largely unknown, it is interesting to note that the multitude of these peptides are expressed in both males and females of A. burtoni, suggesting a function in reproductive competence could be conserved in vertebrates.…”

Section: Crh/ucn Expression and Roles In Peripheral Tissuesmentioning

confidence: 99%

Expression patterns and evolution of urocortin and corticotropin‐releasing hormone genes in a cichlid fish

et al. 2021

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The corticotropin‐releasing hormone and urocortin family of peptides consists of five members in many vertebrates: CRH (crha/crhb in teleosts), CRH2, UCN/UTS1, UCN2, and UCN3. These genes differ in expression pattern, as well as receptor affinity, allowing them to serve a wide range of functions in a variety of species. To better understand the roles of these genes in a single species, we examined their expression patterns in the cichlid fish Astatotilapia burtoni. In situ hybridization to map mRNA expression patterns of crhb, uts1, ucn2, and ucn3 in the brain revealed conserved and distinct spatial features of expression. crhb‐ and uts1‐expressing cells were the most broadly distributed, with several areas of co‐regionalization. ucn3 was less abundant but was found in discrete regions throughout the extent of the brain, with high expression in the cerebellum, while ucn2 was restricted to only a few areas. RT‐PCR showed that while crhb, uts1, and ucn3 are found in several body tissues and widespread throughout the brain, ucn2 is quite restricted in the brain, and crha is only expressed in the eye. Bayesian phylogenetic analyses identified detailed relationships and novel orthologs in the urocortin family. We found evidence for a UCN2 gene loss in some reptiles. Our detailed description of the complete family of genes in the central nervous system of a model organism will inform future studies on the function of these genes in A. burtoni and provides a foundation for comparative studies with teleosts and other vertebrates.

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Section: Crh/ucn Expression and Roles In Peripheral Tissuesmentioning

confidence: 99%

Expression patterns and evolution of urocortin and corticotropin‐releasing hormone genes in a cichlid fish

et al. 2021

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“…There is evidence that this system exists in ovarian compartments of other species, including humans [58]. Moreover, its actions may vary between follicles and corpora lutea, or between ligand-R1 and –R2 systems [59, 60]. …”

Section: Luteotropic Factors: Luteinizing Hormone (Lh) and Lh-indmentioning

confidence: 99%

Endocrine and local control of the primate corpus luteum

Stouffer

Bishop

Bogan

et al. 2013

Reproductive Biology

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The primate corpus luteum is a transient endocrine gland that differentiates from the ovulatory follicle midway through the ovarian (menstrual) cycle. Its formation and limited lifespan is critical for fertility, as luteal-derived progesterone is the essential steroid hormone required for embryo implantation and maintenance of intra-uterine pregnancy until the placenta develops. It is well-established that LH and the LH-like hormone, CG, are the vital luteotropic hormones during the menstrual cycle and early pregnancy, respectively. Recent advances, particularly through genome analyses and cellular studies, increased our understanding of various local factors and cellular processes associated with the development, maintenance and repression of the corpus luteum. These include paracrine or autocrine factors associated with angiogenesis (e.g., VEGF), and that mediate LH/CG actions (e.g., progesterone), or counteract luteotropic effects (i.e., local luteolysis; e.g., PGF2α). However, areas of mystery and controversy remain, particularly regarding the signals and events that initiate luteal regression in the non-fecund cycle. Novel approaches capable of gene “knockdown” or amplification”, in vivo as well as in vitro, should identify novel or underappreciated gene products that are regulated by or modulate LH/CG actions to control the functional lifespan of the primate corpus luteum. Further advances in our understanding of luteal physiology will help to improve or control fertility for purposes ranging from preservation of endangered primate species to designing novel ovary-based contraceptives and treating ovarian disorders in women. R01 HD020869, R01 HD042000, U54 HD018185, U54 HD055744, P51 OD011092, T32 HD007133, Bayer Schering Pharma AG.

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“…In vitro studies show that the activity of NK cells can be improved by IL-2 (Yata et al, 2009) and IFN-g (Charley et al, 1985), but can be inhibited by IL-4 (Canning and Knoblock, 1992). Therefore, the increase of serum IFN-g may have resulted in the percentage of NK cells being increased after transport in our experiment.…”

Section: Inhibition Effect Of Transport Stress On Lymphocyte Subsetsmentioning

confidence: 99%

Effect of transport stress on peripheral blood lymphocyte subsets and Th cytokines in pigs

Ma¹,

Zhong²,

Huangrong³

et al. 2013

ACTA VET-BEOGRAD

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In order to investigate transport stress on porcine peripheral blood lymphocyte subsets and Th cytokines, blood samples were collected from pigs before and after transport. Creatine kinase (CK), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), glucose, and cortisol in the serum were measured. The number of leukocytes and lymphocytes, percentages of lymphocyte subsets, as well as Th cell cytokines level and their mRNA expression were detected, respectively. After transport, the level of CK, glucose and interleukin (IL)-4 increased significantly (p<0.01), LDH, Th memory cells, natural killer and interferon (IFN)-γ increased significantly (p<0.05), cortisol, number of leukocytes and lymphocytes decreased (p<0.01), percentages of γ δ T cells, naïve Th cells and cytotoxic T lymphocytes decreased significantly (p<0.05). The mRNA expressions IL-2 and IFN-γ were down regulated, p<0.01 and p<0.05, respectively. While IL-4, IL-6, and IL-10 were up regulated, but only IL-10 displayed a highly significant difference (p<0.01). These data suggested that transport could cause immune suppression in pigs, which influences cellular immunity more than humoral immunity, and humoral immunity may play an important role in transport stress. Proper measures should be taken to reduce susceptibility of infection after transport

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Suppression of progesterone production by stresscopin/urocortin 3 in cultured human granulosa-lutein cells

Abstract: The present results suggest that the SCP/CRHR2 system is present in human ovaries and treatment with SCP/Ucn3 inhibits progesterone production by cultured granulosa-lutein cells through interaction with CRHR2.

Cited by 7 publications

References 33 publications

Expression patterns and evolution of urocortin and corticotropin‐releasing hormone genes in a cichlid fish

Expression patterns and evolution of urocortin and corticotropin‐releasing hormone genes in a cichlid fish

Endocrine and local control of the primate corpus luteum

Effect of transport stress on peripheral blood lymphocyte subsets and Th cytokines in pigs

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