Suppression of PBAN receptor expression reduces fecundity in the fall armyworm, <i>Spodoptera frugiperda</i>

Park, Youngjin; Vatanparast, Mohammad

doi:10.1002/arch.21897

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…In addition, RNAi of PBAN expression led to reduced transcript levels associated with sex pheromone biosynthesis in Spodoptera liture 34 . Similarly, RNAi of PBAN receptor gene led to a significant reduction in fecundity, which presumably suppressed the pheromone biosynthetic machinery in S. frugiperda 35 . These sugggest that Fo-PBAN mediates the biosynthetic machinery by activating biosynthetic enzymes and up-regulating their gene expressions in the production of the aggregation pheromone.…”

Section: Discussionmentioning

confidence: 99%

A novel physiological function of pheromone biosynthesis-activating neuropeptide in production of aggregation pheromone

Khan

Kim

Sung

et al. 2023

Sci Rep

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The western flower thrips, Frankliniella occidentalis, is an insect pest, and its aggregation pheromone (AP) plays a crucial role in the recruitment of both sexes. A novel pheromone biosynthesis-activating neuropeptide (PBAN)-like gene is encoded in F. occidentalis genome, but its physiological function has yet to be elucidated. This study hypothesized the physiological role played by PBAN in mediating AP production. AP has been known to be produced only by male adults in F. occidentalis. Surprisingly, our extraction of headspace volatiles contained two AP components in females as well as in males with similar composition. PBAN injection elevated the AP production whereas RNA interference (RNAi) of the gene expression suppressed the AP production in both sexes. A biosynthetic pathway to produce AP components were predicted and the enzymes catalyzing the main steps were confirmed in their expressions. Individual RNAi treatments of these genes significantly suppressed AP production. RNAi of PBAN gene downregulated the expressions of these biosynthesis-associated genes in both sexes. These results suggest that the novel neuropeptide acts as PBAN mediating AP production through stimulating its biosynthetic machinery in F. occidentalis.

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Section: Discussionmentioning

confidence: 99%

A novel physiological function of pheromone biosynthesis-activating neuropeptide in production of aggregation pheromone

Khan

Kim

Sung

et al. 2023

Sci Rep

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“…Functional analyses of PBANR in moths have mainly demonstrated the reactivity of PBANR to PBAN. However, in a few species such as P. xylostella and S. frugiperda, the function of PBANR was analyzed after PBANR suppression by RNAi in the pheromone glands of female adults [16,47]. In this study, prior to RNAi treatment, we measured the diurnal expression of MviPBANR-C in response to MviPBAN in the pheromone glands of M. vitrata.…”

Section: Discussionmentioning

confidence: 99%

“…Here, we focused on RNAi regions on PBANR sequences in three moth species. In the case of P. xylostella and S. frugiperda, RNAi was performed on the TM region containing ECL, which is important for ligand binding [16,47]. However, RNAi suppression was performed on the C-terminal tail of MviPBANR-C, which is different from that of MviPBANR-B.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Pheromone Biosynthesis Activating Neuropeptide Receptor Isoforms in Maruca vitrata

Hyun

Kim

Lee

2023

Cells

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Insect sex pheromones are volatile chemicals that induce mating behavior between conspecific individuals. In moths, sex pheromone biosynthesis is initiated when pheromone biosynthesis-activating neuropeptide (PBAN) synthesized in the suboesophageal ganglion binds to its receptor on the epithelial cell membrane of the pheromone gland. To investigate the function of PBAN receptor (PBANR), we identified two PBANR isoforms, MviPBANR-B and MviPBANR-C, in the pheromone glands of Maruca vitrata. These two genes belong to G protein-coupled receptors (GPCRs) and have differences in the C-terminus but share a 7-transmembrane region and GPCR family 1 signature. These isoforms were expressed in all developmental stages and adult tissues. MviPBANR-C had the highest expression level in pheromone glands among the examined tissues. Through in vitro heterologous expression in HeLa cell lines, only MviPBANR-C-transfected cells responded to MviPBAN (≥5 µM MviPBAN), inducing Ca2+ influx. Sex pheromone production and mating behavior were investigated using gas chromatography and a bioassay after MviPBANR-C suppression by RNA interference, which resulted in the major sex pheromone component, E10E12-16:Ald, being quantitatively reduced compared to the control, thereby decreasing the mating rate. Our findings indicate that MviPBANR-C is involved in the signal transduction of sex pheromone biosynthesis in M. vitrata and that the C-terminal tail plays an important role in its function.

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“…The successful achievements in gene knockdown or silencing with significant adverse effects on reproduction make RNAi a promising tool against S. frugiperda. Recent studies have shown that the fertility and the total number of eggs laid per adult female of S. frugiperda were decreased by the particular regulation of Sf-PBANr (neuropeptide receptor activating pheromone biosynthesis) by RNA interference ( Park and Vatanparast, 2022 ). Therefore, it is advisable to improve RNAi efficiency by focusing on gene functional studies for the identification of key genes that govern S. frugiperda reproduction and ways of delivery to sustain its applicability.…”

Section: Methodsmentioning

confidence: 99%

RNA interference (RNAi) applications to the management of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae): Its current trends and future prospects

Kebede

Fite

2022

Front. Mol. Biosci.

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The fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) is among the invasive insect pests that damages maize and sorghum, the high-priority crops in newly colonized agro-ecologies, including African contexts. Owing to the increasing infestation of the pest and the limitations of current conventional methods for its management, there is a call for discovering advanced pest management approaches. RNA interference (RNAi) is an emerging molecular tool showing flexible potential for the management of S. frugiperda. We conducted a search of the recent application of RNAi literature using Google Scholar and Mendeley to find advanced papers on S. frugiperda management using RNAi molecular tools that led to growth inhibition, developmental aberrations, reduced fecundity, and mortality, mainly by disruption of normal biological processes of the pest. Although efforts have been made to accelerate the utility of RNAi, many factors limit the efficiency of RNAi to achieve successful control over S. frugiperda. Owing to RNAi’s potential bioactivity and economic and ecological acceptability, continued research efforts should focus on improving its broad applicability, including field conditions. Screening and identification of key target genes should be a priority task to achieve effective and sustainable management of this insect via RNAi. In addition, a clear understanding of the present status of RNAi utilization in S. frugiperda management is of paramount importance to improve its efficiency. Therefore, in this review, we highlight the biology of S. frugiperda and the RNAi mechanism as a foundation for the molecular management of the pest. Then, we discuss the current knowledge of the RNAi approach in S. frugiperda management and the factors affecting the efficiency of RNAi application. Finally, the prospects for RNAi-based insect pest management are highlighted for future research to achieve effective management of S. frugiperda.

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Suppression of PBAN receptor expression reduces fecundity in the fall armyworm, Spodoptera frugiperda

Cited by 5 publications

References 38 publications

A novel physiological function of pheromone biosynthesis-activating neuropeptide in production of aggregation pheromone

A novel physiological function of pheromone biosynthesis-activating neuropeptide in production of aggregation pheromone

Functional Analysis of Pheromone Biosynthesis Activating Neuropeptide Receptor Isoforms in Maruca vitrata

RNA interference (RNAi) applications to the management of fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae): Its current trends and future prospects

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