Suppression of Neutrophil Superoxide Production by Conventional Peritoneal Dialysis Solution

Bl, Ing; Dk, Gupta; Zm, Nawab; Fq, Zhou; Ma, Rahman; Jt, Daugirdas

doi:10.1177/039139888801100508

Cited by 64 publications

(31 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, several in vitro experiments have demonstra ted that fresh peritoneal dialysate reduces the viability of lymphocytes [3], polymorphonuclear leukocytes (PMNL) [4] and peritoneal macrophages [5], inhibits the phagocytosis and bacterial killing of PMNL [6] and peri toneal macrophages [4] and impairs their respiratory and oxidative metabolism [7,8], In contrast, little is known about the effects of dialysates on the ability of leukocytes to generate inflammatory mediators.…”

Section: Introductionmentioning

confidence: 99%

Leukotriene B<sub>4</sub> and Tumor Necrosis Factor Release from Leukocytes: Effect of Peritoneal Dialysate

Jörres

Gahl

et al. 1991

Nephron

View full text Add to dashboard Cite

The effect of peritoneal dialysate on the capacity of peripheral blood polymorphonuclear (PMNL) and mononuclear leukocytes (MNC) to release leukotriene B₄ (LTB₄) and tumor necrosis factor alpha (TNFα) was investigated in vitro. Following density gradient separation, aliquots of 5 × 10⁶ PMNL or MNC were incubated in peritoneal dialysis fluid containing 1.5% glucose or Hanks’ buffer ( = control) for 1–2 h at 37^°C. TNFα and LTB₄production was stimulated with Escherichia coli lipopolysaccharide (LPS) and calcium ionophore A23187, respectively. MNC incubated in buffer and LPS produced (mean ± SD) 1,006 ± 522 pg TNFα/5 × 10⁶ cells; no significant amounts of TNFα were detectable in the presence of dialysate. An inhibition of TNFα release was also observed in MNC exposed to bicarbonate-buffered dialysates (pH 7.40) and 4.25% and 1.5% glucose solution with physiologic osmolality. Incubation of PMNL in Hanks’ buffer followed by A23187 stimulation led to production of 29.1 ± 19.2 ng LTB₄/5 × 10⁶ cells, whereas glucose-incubated cells were refractory to ionophore stimulation ( < 0.l ng LTB₄/5 × 10⁶ cells). The failure of dialysate-exposed leukocytes to release inflammatory mediators in response to adequate stimuli may contribute to the impairment of cellular host defense in the setting of continuous ambulatory peritoneal dialysis.

show abstract

Section: Introductionmentioning

confidence: 99%

Leukotriene B<sub>4</sub> and Tumor Necrosis Factor Release from Leukocytes: Effect of Peritoneal Dialysate

Jörres

Gahl

et al. 1991

Nephron

View full text Add to dashboard Cite

show abstract

“…The low pH of lactate-buffered PDF is known to sup press several important functional aspects o f peripheral and peritoneal leukocytes [34][35][36][37]. As far as the immune function of PM 0 after preincubation in acidic versus pHadjusted PDF is concerned, our data provide additional evidence of this detrimental effect of low pH.…”

Section: Discussionmentioning

confidence: 68%

In vitro Effects of Amino-Acid-Based versus Glucose-Based Continuous Ambulatory Peritoneal Dialysis Fluids on Peritoneal Macrophage Function

Schenk

Kiefer

Hübel

et al. 1994

Nephron

View full text Add to dashboard Cite

We studied the in vitro effects of peritoneal dialysis fluids (PDF) containing 1 and 1.5% amino acids (AA) as compared to approximately equiosmolar glucose (GLU)-based PDF (1.5 and 4.25%) and control buffer, respectively, on peritoneal macrophage (PMØ) function. The media were tested at original pH (5.3-5.5) and after pH adjustment to 7.4. PMØ were isolated from the effluents of 10 on continuous ambulatory peritoneal dialysis (CAPD) patients and tested for luminol- and lucigenin-enhanced chemiluminescence (CL), superoxide generation measured by cytochrome c reduction, killing capacity and phagocytosis after incubation (30 min, 37°C) in the PDF used. All AA-based PDF exhibited a statistically significant depressive effect on integral CL response, O^-₂production and bacterial killing of PMØ at pH 7.4 in comparison with pH-adjusted GLU-based PDF of similar osmolality and buffer. Exposure of PMØ to acidic AA-based media did not result in a significantly different suppression of the oxidative metabolism and the killing capacity as compared to fresh GLU-based fluids. Phagocytosis of PMØ did not show significant differences after incubation in the solutions studied. Thus, the AA-based PDF employed compromise the oxidative metabolism and the killing capacity of PMØ at pH 7.4 in vitro significantly more than GLU-based fluids. Since pH-identical and almost equiosmolar PDF were compared, the specific composition of the AA-based fluids, especially the high content of lactate and several essential AA, could be responsible for this detrimental impact.

show abstract

“…during its dwell time in the perito neal cavity. Other investigators have corrobo rated these findings and have presented evi dence that incubation in unused dialysis fluid inhibits the generation of oxygen free radicals by activated peripheral blood leukocytes as well as by peritoneal macrophages [24][25][26], It was Topley et al [16] who conclusively dem onstrated that the inhibitory effect on the phagocytic cell function is mainly due to low pH of lactate-containing peritoneal dialysis solutions.…”

Section: Dialysate Ph and Phagocytic Cell Functionmentioning

confidence: 99%

Impact of Terminal Heat Sterilization on the Quality of Peritoneal Dialysis Solutions

Martis

Henderson²

1997

Blood Purif

View full text Add to dashboard Cite

In order to meet the sterility requirements imposed by the various regulatory bodies around the world, peritoneal dialysis solutions are terminally heat-sterilized at an acid pH. There is growing concern that both acid pH and the glucose degradation products formed during terminal heat sterilization adversely affect the quality of the therapy provided to the end-stage renal failure patient on peritoneal dialysis. Acid pH and glucose degradation products are thought to contribute to impaired host defense and hence to increased risk of infection, loss of ultrafiltration, and peritoneal fibrosis. On the other hand, peritoneal dialysis solutions prepared by aseptic processing are devoid of in vitro cytotoxicity and hence are considered more biocompatible than heat-sterilized solutions. With new technologies permitting aseptic processing to achieve sterility assurance levels approaching solutions manufactured by terminal heat sterilization, it is possible to produce solutions that are more biocompatible than heat-sterilized products without increasing the risk of microbial contamination.

show abstract

Suppression of Neutrophil Superoxide Production by Conventional Peritoneal Dialysis Solution

Cited by 64 publications

References 9 publications

Leukotriene B<sub>4</sub> and Tumor Necrosis Factor Release from Leukocytes: Effect of Peritoneal Dialysate

Leukotriene B<sub>4</sub> and Tumor Necrosis Factor Release from Leukocytes: Effect of Peritoneal Dialysate

In vitro Effects of Amino-Acid-Based versus Glucose-Based Continuous Ambulatory Peritoneal Dialysis Fluids on Peritoneal Macrophage Function

Impact of Terminal Heat Sterilization on the Quality of Peritoneal Dialysis Solutions

Contact Info

Product

Resources

About