Suppression of myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish

Lee, Chiou-Yueh; Hu, Shao‐Yang; Gong, Hong‐Yi; Chen, Mark Hung‐Chih; Lu, June; Wu, Jen‐Leih

doi:10.1016/j.bbrc.2009.07.110

Cited by 96 publications

(65 citation statements)

References 15 publications

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“…Direct evidence of myostatin function in fish, however, is seriously lacking. A few studies have overexpressed or attempted to disrupt the production and/or bioavailability of individual paralogs with mixed results (Xu et al 2003, Amali et al 2004, Rodgers & Garikipati 2008, Lee et al 2009). This includes the generation of transgenic rainbow trout overexpressing follistatin, a myostatin binding protein, which developed hypermuscularity that resembled myostatin null phenotypes in mammals (Medeiros et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Myostatin inhibits myosatellite cell proliferation and consequently activates differentiation: evidence for endocrine-regulated transcript processing

Garikipati¹,

Rodgers²

2012

Journal of Endocrinology

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Myostatin is a potent negative regulator of muscle growth in mammals. Despite high structural conservation, functional conservation in nonmammalian species is only assumed. This is particularly true for fish due to the presence of several myostatin paralogs: two in most species and four in salmonids . Rainbow trout are a rich source of primary myosatellite cells as hyperplastic muscle growth occurs even in adult fish. These cells were therefore used to determine myostatin's effects on proliferation whereas our earlier studies reported its effects on quiescent cells. As in mammals, recombinant myostatin suppressed proliferation with no changes in cell morphology. Expression of MSTN-1a was several fold higher than the other paralogs and was autoregulated by myostatin, which also upregulated the expression of key differentiation markers: Myf5, MyoD1, myogenin, and myosin light chain. Thus, myostatin-stimulated cellular growth inhibition activates rather than represses differentiation. IGF-1 stimulated proliferation but had minimal and delayed effects on differentiation and its actions were suppressed by myostatin. However, IGF-1 upregulated MSTN-2a expression and the processing of its transcript, which is normally unprocessed. Myostatin therefore appears to partly mediate IGF-stimulated myosatellite differentiation in rainbow trout. This also occurs in mammals, although the IGF-stimulated processing of MSTN-2a transcripts is highly unique and is indicative of subfunctionalization within the gene family. These studies also suggest that the myokine's actions, including its antagonistic relationship with IGF-1, are conserved and that the salmonid gene family is functionally diverging.

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Section: Introductionmentioning

confidence: 99%

Myostatin inhibits myosatellite cell proliferation and consequently activates differentiation: evidence for endocrine-regulated transcript processing

Garikipati¹,

Rodgers²

2012

Journal of Endocrinology

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“…Although the roles of both MSTN and GDF11 have been explored by functional studies in vertebrates (Lee et al, 2009;McPherron et al, 1999;Wu et al, 2003), the physiological functional role of iMSTN/GDF11 is still not entirely understood. Previous findings reported that the iMstn/Gdf11 gene showed widespread tissue expression, suggesting that, unlike the mammalian orthologs, its physiological significance might not be restricted to specific tissues (Covi et al, 2008;Kim et al, 2004;MacLea et al, 2010;Saina and Technau, 2009).…”

Section: Introductionmentioning

confidence: 99%

Growing backwards: an inverted role for the shrimp ortholog of vertebrate myostatin and GDF11

Santis

Wade

Jerry

et al. 2011

Journal of Experimental Biology

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“…Suppression of myostatin activity has been evaluated in some species of fishes, and studies have shown that its ability to inhibit growth and increase muscle mass is primarily through hyperplasia, hypertrophy, or both (Xu et al, 2003;Acosta et al, 2005;Lee et al, 2009;Chisada et al, 2011). This justifies interest in this factor from a production point of view.…”

Section: Introductionmentioning

confidence: 99%

Morphology and muscle gene expression in GIFT and Supreme Nile tilapia varieties reared in two cultivation systems

et al. 2017

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ABSTRACT. Tissue growth in most fishes occurs by muscular hyperplasia and hypertrophy, which are influenced by different regulatory factors, such as myostatin. The current study evaluated the influence of cultivation in hapas and earthen ponds on the diameter of white muscle fibers and on the myostatin (MSTN-1) gene in GIFT and Supreme varieties of tilapia. Fish of both varieties were reared for 204 days and then divided into four developmental stages. White muscle samples, corresponding to 100 fibers per slide, were collected from the middle region of fish of each variety and cultivation system, and were measured and divided into two classes representing hyperplasia and hypertrophy. Samples were subjected to real-time PCR to analyze gene expression. Hyperplasia decreased during the developing stages, coupled with increased hypertrophy. There was a higher rate of hypertrophy in fish raised in earthen ponds when compared to those raised in hapas, during juvenile and developing phases, and greater hypertrophic growth was observed in GIFT specimens when compared to Supreme specimens in earthen ponds. Since increased MSTN-1 gene expression was observed in GIFT specimens during the developing phase in pond cultivations, and in Supreme tilapia in hapas, MSTN-1 expression is related to greater hypertrophy. These results demonstrate the capacity for increased muscle growth in earthen pond cultivation in which the GIFT variety developed best. How the environment affects the growth of different tilapia varieties may be employed to optimize culture management and genetic improvement programs. Further investigations should aim to describe mechanisms affecting muscle growth and development.

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Suppression of myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish

Cited by 96 publications

References 15 publications

Myostatin inhibits myosatellite cell proliferation and consequently activates differentiation: evidence for endocrine-regulated transcript processing

Myostatin inhibits myosatellite cell proliferation and consequently activates differentiation: evidence for endocrine-regulated transcript processing

Growing backwards: an inverted role for the shrimp ortholog of vertebrate myostatin and GDF11

Morphology and muscle gene expression in GIFT and Supreme Nile tilapia varieties reared in two cultivation systems

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