Suppression of methanogenesis in cellulose-fed microbial fuel cells in relation to performance, metabolite formation, and microbial population

Rismani-Yazdi, Hamid; Carver, Sarah M.; Christy, Ann D.; Yu, Zhongtang; Bibby, Kyle; Peccia, Jordan; Tuovinen, Olli H.

doi:10.1016/j.biortech.2012.10.137

Cited by 80 publications

(45 citation statements)

References 33 publications

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“…Although urban wastewater has been the most common biodegradable fuel tested in METs, alternative organic matter sources such as cellulose [23], food industry residues [24], brewery wastewater [25], cheese wastewater [26], or root exudates [27] have been extensively tested in the last decade.…”

Section: Introductionmentioning

confidence: 99%

Strategies for Reducing the Start-up Operation of Microbial Electrochemical Treatments of Urban Wastewater

et al. 2015

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Abstract:Microbial electrochemical technologies (METs) constitute the core of a number of emerging technologies with a high potential for treating urban wastewater due to a fascinating reaction mechanism-the electron transfer between bacteria and electrodes to transform metabolism into electrical current. In the current work, we focus on the model electroactive microorganism Geobacter sulfurreducens to explore both the design of new start-up procedures and electrochemical operations. Our chemostat-grown plug and play cells, were able to reduce the start-up period by 20-fold while enhancing chemical oxygen demand (COD) removal by more than 6-fold during this period. Moreover, a filter-press based bioreactor was successfully tested for both acetate-supplemented synthetic wastewater and real urban wastewater. This proof-of-concept pre-pilot treatment included a microbial electrolysis cell (MEC) followed in time by a microbial fuel cell (MFC) to finally generate electrical current of ca. 20 A¨m´2 with a power of 10 W¨m´2 while removing 42 g COD day´1¨m´2.The effective removal of acetate suggests a potential use of this modular technology for treating acetogenic wastewater where Geobacter sulfurreducens outcompetes other organisms.

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Section: Introductionmentioning

confidence: 99%

Strategies for Reducing the Start-up Operation of Microbial Electrochemical Treatments of Urban Wastewater

et al. 2015

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“…Many researchers have reported methane production in the electricity-producing process of MFCs, and the presence of methanogens in MFC systems has been confirmed via microscopic and PCR-based approaches [2,9,21,29,33]. For example [9], observed methane accumulation in the anode chamber headspace of an MFC, and 113.5 ml methane was produced in the electricity production of an MFC with alkaline-pretreated sludge as fuel in our previous study [29].…”

Section: Introductionmentioning

confidence: 99%

“…In the operation of MFCs, methanogenesis is a common phenomenon, particularly when inoculated with mixed anaerobic sludge, since the growth conditions for methanogens are similar to those of exoelectrogens [2,21,33]. Many researchers have reported methane production in the electricity-producing process of MFCs, and the presence of methanogens in MFC systems has been confirmed via microscopic and PCR-based approaches [2,9,21,29,33].…”

Section: Introductionmentioning

confidence: 99%

Relationship of methane and electricity production in two-chamber microbial fuel cell using sewage sludge as substrate

Xiao

Han

Liu

et al. 2014

International Journal of Hydrogen Energy

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RelationshipSewage sludge Two-chamber microbial fuel cell a b s t r a c tThe relationship of methane and electricity production from sewage sludge in a twochamber microbial fuel cell (MFC) was studied. The results showed that methane production in the anode chamber could enhance the electricity production from sewage sludge, and the output voltage of the MFC with methane production (0.505e0.600 V) was higher than that of the MFC without it (0.506e0.576 V) in the stable electricity-producing stage. The polarization curves analysis of the two MFCs suggested that methane production could improve the performance characteristics of the MFC. Simultaneous methane and electricity production from sludge in the two-chamber MFC could maintain the mixed sludge in a suitable pH range in the anode chamber for electricity production. Meanwhile, simultaneous methane and electricity production could enhance the hydrolysis of sludge, which increased the reduction of sludge concentration (about 8.31% VSS) and offered more substrates to alleviate the competition between methane and electricity production.Additionally, the addition of 2-Bromoethanesulfonate (BES) could substantially affect the dominant archaea but had little effect on the dominant bacteria in the anode chamber.

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“…Commonly, they are recognised as a hydrolytic fermenter of carbohydrates in anaerobic habitats (Thomas et al, 2011;Rismani-Yazdi et al, 2013). Filamentous Bacteroidetes was 70#|#P a g e # % found in activated sludge and has the ability to take up glucose and propionate under aerobic conditions (Kragelund et al, 2008).…”

Section: Microbial Architecturementioning

confidence: 99%

“…This may suggest that Methanosaeta relies on acetate produced by acetogenesis under simple substrate (VFAs) but by fermentation under complex substrate (carbohydrate or protein). Methanobacterium and Methanolinea are also linked to presumed fermenters Anaerolinea/Bacteroidales in glucose granules due to the production of H 2 /CO 2 from glucose hydrolysis (Sekiguchi et al, 2003;Thomas et al, 2011;Rismani-Yazdi et al, 2013). The correlation between bacteria and archaea in the inner layers (on the inside of acetogenic zone) may be more difficult due to that archaea are possibly related to multiple bacteria from different trophic groups and acetate and H 2 /CO 2 are produced from fermentation and acetogenesis.…”

Section: Relationship Between Trophic Groups At Varying Granule Depthmentioning

confidence: 99%

Microbial Ecology of Fermentative Microbes in Anaerobic Granules

Yang¹

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School of Chemical EngineeringII"|"P a g e " % AbstractGranules are large, self-supporting biofilms that form naturally in high-rate anaerobic treatment systems and are extremely important to reactor functionality. The biofilm structure has functional and phylogenetic layering, important both scientifically and to function.Fermenters, found on the surface of anaerobic granules, are associated with granule strength and uptake of the primary substrate. Compared to acetogens and methanogens, very limited work has been done on fermenters, particularly in granules. Due to their high diversity and difficulty in selective isolation, fermenters are best studied through culture-independent techniques. Until now, the microbial distribution could only be analysed through sectioning and microscopic analysis with fluorescent in situ hybridization. The whole granule could be analysed by DNA extraction and microbial community profiling methods but this did not provide spatial information. This thesis develops a method to remove microbes selectively from successive spatial layers through hydraulic shearing and demonstrates its application on anaerobic granules of three different types (collected from brewery, cannery and dairy wastewater treatment plants). Outer layers, in particular, could be selectively sheared as confirmed by FISH and TRFLP. Further analysis with 454 pyrosequencing showed that a shift in dominant population from presumptive fermenters (such as Bacteroidales and Anaerolinea) in outer layers to syntrophs (such as Syntrophomonas and Geobacter) in inner layers, with progressive changes through the depth. The method was further leveraged through covariance based deep metagenomic sequencing, with metagenomic analysis used so far to align phylogenetic information. This leveraged the shear based method to provide covariance information for the metagenomic analysis. Shear based phylogenetic and metagenomic aligned well internally and with cryosection-FISH analysis. Information provided could be used with more specific probes (particularly Bacteroidales and Anaerolinea) to confirm that these organisms were key fermenters and highly abundant in outer layers. This study indicated that fermenters were a relatively diverse but discrete population within the granule. Further analysis of the metagenomic information is required to identify roles of specific microbes. The phylogenetic approach was also used in a reactor study with different feeds (gelatine, glucose, VFA) to identify how the microbial population shifted during growth of fermenters. While physical strength was not influenced, the III"|"P a g e " % dominant fermentative groups were strongly impacted by the feed matrix, and inner layer community less changed, but still substantially affected.

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Suppression of methanogenesis in cellulose-fed microbial fuel cells in relation to performance, metabolite formation, and microbial population

Cited by 80 publications

References 33 publications

Strategies for Reducing the Start-up Operation of Microbial Electrochemical Treatments of Urban Wastewater

Strategies for Reducing the Start-up Operation of Microbial Electrochemical Treatments of Urban Wastewater

Relationship of methane and electricity production in two-chamber microbial fuel cell using sewage sludge as substrate

Microbial Ecology of Fermentative Microbes in Anaerobic Granules

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