Suppression of Lα/Lβ Phase Coexistence in the Lipids of Pulmonary Surfactant

Fritz, Jonathan R.; Loney, Ryan W.; Hall, Stephen B.; Tristram-Nagle, Stephanie

doi:10.1016/j.bpj.2020.12.008

Cited by 3 publications

(4 citation statements)

References 70 publications

(103 reference statements)

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“…The molar ratio of SP-C to SP-B in natural and clinical surfactants as determined by UV absorption varies greatly: Alveofact, 11:1; native bovine, 13:1; Survanta, 55:1; native porcine, 9:1; Curosurf, 14:1 . Colorimetric assays indicate the SP-C:SP-B molar ratios for the bovine clinical surfactants are BLES, 6:1 and CLSE (Infasurf), 5:1. , This data indicates that SP-C is normally considerably more abundant, particularly if it is assumed that most of the SP-B is localized in hexamers composed of SP-B dimers. Although direct evidence is lacking, it should be considered that SP-C could also form multimer structures.…”

Section: How Does Pulmonary Surfactant Adsorb Rapidly?mentioning

confidence: 95%

“…However, increasing the temperature to the physiological range would decrease the number of gel phase domains in monolayers. With Infasurf bilayers, WAXS studies document that the nanodomains almost disappear at 37 °C . The presence of SP-B plus SP-C accelerates this loss.…”

Section: How Does Pulmonary Surfactant Reduce Surface Tension To Low ...mentioning

confidence: 96%

“…With Infasurf bilayers, WAXS studies document that the nanodomains almost disappear at 37 °C. 349 The presence of SP-B plus SP-C accelerates this loss. Small angle X-ray scattering (SAXS) analyses (see Section 4.4.1 for technical details) of porcine natural and lipid extract surfactant bilayers find no evidence for the LO phase when heated to 40 °C, although this thicker lamellar phase is still detected with the total porcine surfactant PL fraction.…”

Section: Surfactant Monolayers As Compositesmentioning

confidence: 99%

“…243 Colorimetric assays indicate the SP-C:SP-B molar ratios for the bovine clinical surfactants are BLES, 6:1 and CLSE (Infasurf), 5:1. 349,350 This data indicates that SP-C is normally considerably more abundant, particularly if it is assumed that most of the SP-B is localized in hexamers composed of SP-B dimers. Although direct evidence is lacking, it should be considered that SP-C could also form multimer structures.…”

Section: Role Of Sp-c In Adsorptionmentioning

confidence: 99%

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Pulmonary Surfactant: A Mighty Thin Film

Possmayer,

Zuo,

Veldhuizen

et al. 2023

Chem. Rev.

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Pulmonary surfactant is a critical component of lung function in healthy individuals. It functions in part by lowering surface tension in the alveoli, thereby allowing for breathing with minimal effort. The prevailing thinking is that low surface tension is attained by a compression-driven squeeze-out of unsaturated phospholipids during exhalation, forming a film enriched in saturated phospholipids that achieves surface tensions close to zero. A thorough review of past and recent literature suggests that the compression-driven squeeze-out mechanism may be erroneous. Here, we posit that a surfactant film enriched in saturated lipids is formed shortly after birth by an adsorptiondriven sorting process and that its composition does not change during normal breathing. We provide biophysical evidence for the rapid formation of an enriched film at high surfactant concentrations, facilitated by adsorption structures containing hydrophobic surfactant proteins. We examine biophysical evidence for and against the compressiondriven squeeze-out mechanism and propose a new model for surfactant function. The proposed model is tested against existing physiological and pathophysiological evidence in neonatal and adult lungs, leading to ideas for biophysical research, that should be addressed to establish the physiological relevance of this new perspective on the function of the mighty thin film that surfactant provides.

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Section: How Does Pulmonary Surfactant Adsorb Rapidly?mentioning

confidence: 95%

Section: How Does Pulmonary Surfactant Reduce Surface Tension To Low ...mentioning

confidence: 96%

Section: Surfactant Monolayers As Compositesmentioning

confidence: 99%

Section: Role Of Sp-c In Adsorptionmentioning

confidence: 99%

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