Suppression of Leuconostoc mesenteroides During Isolation of Lactobacilli

Mundt, J. Orvin; Hammer, J. L.

doi:10.1128/aem.14.6.1044-1044.1966

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“…Primary medium. Rogosa's SL broth was prepared with 0.04% cycloheximide and with 10% less water than specified, for use at 45 C. To restrict overgrowth by Leuconostoc mesenteroides at 32 C, 95% ethyl alcohol was added to comprise 7% of the same medium after boiling, acidification, and cooling (19). The media were tubed into sterile screw-capped tubes in 9.0-and 4.5-ml quantities.…”

Section: Methodsmentioning

confidence: 99%

Lactobacilli on Plants

Mundt¹,

Hammer²

1968

Applied Microbiology

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The distribution, enumeration, and identification of lactobacilli on vegetable plants were studied in an area described geographically as being subtropical and moist. The lactobacilli were obtained, by means of quantitative enrichment procedures in Rogosa's SL broth, from 35.3% of all samples incubated at 32 C, and from 15.4%, of the samples incubated at 45 C. Less than 10 lactobacilli/g of plant material were enumerated in 54% of all positive samples. The lactobacilli were found much less frequently and in lower numbers than were streptococci or Leuconostoc mesenteroides. The most frequently isolated lactobacillus was very simular to, but not identical with, Lactobacillus fermenti. It was aerogenic, grew well at both 15 and 45 C, fermented arabinose, lactose, and sucrose, and liberated ammonia from arginine. Of the identified species, L. plantarum, L. fermenti, and L. brevis were the most frequently isolated, whereas L. casei, L. viridescens, L. cellobiosus, L. salivarius, and L. buchneri were obtained from small numbers of samples. The widespread but sporadic distribution of lactobacilli in low numbers seems to indicate that these organisms do not normally thrive on plant surfaces. A ternary cycle, beginning with intestinal waste, followed by mechanical distribution to and among plants, and return to the host via the oral cavity, is suggested.

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Section: Methodsmentioning

confidence: 99%