Suppression of intestinal immunity through silencing of TCTP by RNAi in transgenic silkworm, Bombyx mori

Hu, Cuimei; Wang, Fei; Ma, Sanyuan; Li, Xianyang; Song, Liang; Hua, Xiaoting; Xia, Qingyou

doi:10.1016/j.gene.2015.07.089

Cited by 11 publications

(11 citation statements)

References 41 publications

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“…From the RNA‐seq results, 41 genes in the immune pathway showed significant expression differentiation (Fig. A), indicating that BmTCTP plays an important role in intestinal immune response, as reported before (Hu et al ., ). The midgut is a major player in dietary digestion involving columnar cells and goblet cells.…”

Section: Discussionmentioning

confidence: 97%

CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori

Liu

Lin

et al. 2018

Insect Science

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The translationally controlled tumor protein (TCTP) is a highly conserved and multifunctional protein with activities ranging from cytoskeletal regulation to transcription regulation in numerous organisms. In insects, TCTP is essential for cell growth and proliferation. Recently, TCTP has been reported to affect the innate intestinal immune pathway in the Bombyx mori silkworm, a lepidopteran model insect. However, the comprehensive physiological roles of TCTP in the silkworm remain poorly understood. Here, we performed functional analysis of BmTCTP by using a binary transgenic CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/RNA-guided CRISPER-associated protein 9 nucleases) system. Disruption of BmTCTP led to developmental arrestment and subsequent lethality in third instar larvae. Histological analysis revealed that growth impairment originated from decreased cell size, and the proliferation and differentiation of intestinal epithelial cells were also affected. RNA-seq analysis revealed that genes involved in carbohydrate metabolism, lipid metabolism and digestive system pathways were significantly affected by BmTCTP depletion. Together, the results demonstrated that BmTCTP plays a key role in controlling larval growth and development.

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Section: Discussionmentioning

confidence: 97%

CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori

Liu

Lin

et al. 2018

Insect Science

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“…Further, fortilin TCTP is secreted by cells, circulates in the blood, and serves as a viable surrogate marker for in vivo apoptosis [4]. Finally, in fish [130], shrimp [43, 45, 47–49, 52, 53], and silkworms [154, 155], fortilin TCTP participates in host defense against bacterial and viral pathogens through its ability to (a) recognize, bind, and facilitate degradation of the invaders when secreted into the extracellular space and (b) protect immune cells against pathogen-induced cell death while within the cell. Further research could prove fortilin TCTP to be a viable molecular target for diagnosing and treating various human diseases, including and not limited to atherosclerosis, hypertension, cancer, DM, DN, hepatic injury, and aberrant immunity and host defense (Figure 1).…”

Section: Discussionmentioning

confidence: 99%

“…The same group of investigators generated transgenic silkworms that tissue-specifically overexpressed siRNA against Bm-fortilin TCTP RNA. The transgenic silkworms expressed 30–43% less Bm-fortilin TCTP in the midgut and were more susceptible to Sm and Staphylococcus aureus challenge, mounting a smaller amount of Cecropin A1 [155]. These data suggest that Bm-fortilin TCTP protects the worms against bacterial infection through its binding to bacteria and the facilitation of their phagocytic clearance by the hemocytes as well as induction of the AMP (Fig.…”

Section: Fortilintctp In Invertebrate Diseasesmentioning

confidence: 99%

Fortilin: A Potential Target for the Prevention and Treatment of Human Diseases

Pinkaew

Fujise

2017

Advances in Clinical Chemistry

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Fortilin is a highly conserved 172-amino-acid polypeptide found in the cytosol, nucleus, mitochondria, extracellular space, and circulating blood. It is a multifunctional protein that protects cells against apoptosis, promotes cell growth and cell cycle progression, binds calcium (Ca2+), and has anti-pathogen activities. Its role in the pathogenesis of human and animal diseases is also diverse. Fortilin facilitates the development of atherosclerosis, contributes to both systemic and pulmonary arterial hypertension, participates in the development of cancers, and worsens diabetic nephropathy. It is important for the adaptive expansion of pancreatic β-cells in response to obesity and increased insulin requirement, for the regeneration of liver after hepatectomy, and for protection of the liver against alcohol- and ER stress-induced injury. Fortilin is a viable surrogate marker for in vivo apoptosis, and it plays a key role in embryo and organ development in vertebrates. In fish and shrimp, fortilin participates in host defense against bacterial and viral pathogens. Further translational research could prove fortilin to be a viable molecular target for treatment of various human diseases including and not limited to atherosclerosis, hypertension, certain tumors, diabetes mellitus, diabetic nephropathy, hepatic injury, and aberrant immunity and host defense.

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“…The authors conclude that TCTP is a dual-function protein involved in both the cellular and the humoral immune response of the silkworm. In support of this, the group recently studied the effect of silencing of TCTP by RNAi in a transgenic silkworm (Hu et al 2015). They reported that the antimicrobial capacity of the silkworm decreased, since the expression of the gut antimicrobial peptide was not sufficiently induced during microbial challenge.…”

Section: Tctp In Arthropodsmentioning

confidence: 99%

The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

Bommer

2017

Results and Problems in Cell Differentiation

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The Translational Controlled Tumour Protein TCTP (gene symbol TPT1, also called P21, P23, Q23, fortilin or histamine-releasing factor, HRF) is a highly conserved protein present in essentially all eukaryotic organisms and involved in many fundamental cell biological and disease processes. It was first discovered about 35 years ago, and it took an extended period of time for its multiple functions to be revealed, and even today we do not yet fully understand all the details. Having witnessed most of this history, in this chapter, I give a brief overview and review the current knowledge on the structure, biological functions, disease involvements and cellular regulation of this protein. TCTP is able to interact with a large number of other proteins and is therefore involved in many core cell biological processes, predominantly in the response to cellular stresses, such as oxidative stress, heat shock, genotoxic stress, imbalance of ion metabolism as well as other conditions. Mechanistically, TCTP acts as an anti-apoptotic protein, and it is involved in DNA-damage repair and in cellular autophagy. Thus, broadly speaking, TCTP can be considered a cytoprotective protein. In addition, TCTP facilitates cell division through stabilising the mitotic spindle and cell growth through modulating growth signalling pathways and through its interaction with the proteosynthetic machinery of the cell. Due to its activities, both as an anti-apoptotic protein and in promoting cell growth and division, TCTP is also essential in the early development of both animals and plants. Apart from its involvement in various biological processes at the cellular level, TCTP can also act as an extracellular protein and as such has been involved in modulating whole-body defence processes, namely in the mammalian immune system. Extracellular TCTP, typically in its dimerised form, is able to induce the release of cytokines and other signalling molecules from various types of immune cells. There are also several examples, where TCTP was shown to be involved in antiviral/antibacterial defence in lower animals. In plants, the protein appears to have a protective effect against phytotoxic stresses, such as flooding, draught, too high or low temperature, salt stress or exposure to heavy metals. The finding for the latter stress condition is corroborated by earlier reports that TCTP levels are considerably up-regulated upon exposure of earthworms to high levels of heavy metals. Given the involvement of TCTP in many biological processes aimed at maintaining cellular or whole-body homeostasis, it is not surprising that dysregulation of TCTP levels may promote a range of disease processes, foremost cancer. Indeed a large body of evidence now supports a role of TCTP in at least the most predominant types of human cancers. Typically, this can be ascribed to both the anti-apoptotic activity of the protein and to its function in promoting cell growth and division. However, TCTP also appears to be involved in the later stages of cancer progression, such ...

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Suppression of intestinal immunity through silencing of TCTP by RNAi in transgenic silkworm, Bombyx mori

Cited by 11 publications

References 41 publications

CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori

CRISPR disruption of TCTP gene impaired normal development in the silkworm Bombyx mori

Fortilin: A Potential Target for the Prevention and Treatment of Human Diseases

The Translational Controlled Tumour Protein TCTP: Biological Functions and Regulation

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