Suppression of insulin-like growth factor signalling pathway and collagen expression in keloid-derived fibroblasts by quercetin: its therapeutic potential use in the treatment and/or prevention of keloids

Phan, Toan Thang; See, Patrick; Tran, Elizabeth; Nguyen, T.T.T.; Chan, Sui Yung; Lee, S.T.; Huynh, Hung

doi:10.1046/j.1365-2133.2003.05174.x

Cited by 42 publications

(45 citation statements)

References 41 publications

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“…The stimulatory effect on collagen biosynthesis of [7], [8] and [10] was not restricted to the OI cell line, because similar results were obtained following treatment of normal cells. Since these compounds are of special interest for their potential use in the therapy of OI type I, further study was conducted to determine the mechanism of their action.…”

Section: ------------------------------------------------------------supporting

confidence: 64%

“…2C). At the concentration of 30 μM of [8] and [10], the percentage of synthesized collagen in OI cells was close to that of normal fibroblasts, whereas [7] increased collagen synthesis bỹ 63% of the control. Treatment of cells with different concentrations of [7] did not cause more effective stimulation; therefore, the concentration of 30 μM for all three compounds was used in further experiments.…”

Section: Resultsmentioning

confidence: 91%

“…In contrast, apigenin glycosides 7-O-glucoside [6], 7-Oglucuronide [7], 7-O-methylglucuronide [8] and pectolinarin [10] significantly induced collagen synthesis with little or no effect on the overall protein synthesis. The specific induction of collagen synthesis by [7], [8] and [10] was further demonstrated when collagen synthesis was expressed as a percentage of the total cell protein synthesis (Fig. 2C).…”

Section: Resultsmentioning

confidence: 99%

“…3). The percentage of collagen secreted into the medium was ---------------------------------------------------------------------------------------------------------------- estimated as ~83% for the normal and 82% for OI cells and it did not change after treatment of OI cells with [7]; in the case of [8] and [10] only a slight increase was observed (Fig. 4).…”

Section: Resultsmentioning

confidence: 99%

“…The group is known for its antioxidant, antiradical, anti-inflammatory, anti-microbial as well as anti-allergic effects. Flavonoids and flavonoid-rich extracts have been implicated as beneficial agents in cancer, cardiovascular and neurodegenerative disorders (8) as well as in diseases connected with disrupted collagen metabolism (9)(10)(11). The therapeutic usefulness of flavonoids is still increasing.…”

Section: Introductionmentioning

confidence: 99%

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Stimulation of collagen biosynthesis by flavonoid glycosides in skin fibroblasts of osteogenesis imperfecta type I and the potential mechanism of their action

Gavin¹,

Nazaruk²

2007

Int J Mol Med

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Abstract. The aim of the present study was to identify bioactive compounds stimulating collagen biosynthesis with potential for osteogenesis imperfecta (OI) type I pharmacological therapy. Of the compounds tested, apigenin glycosides 7-O-glucuronide, 7-O-methylglucuronide and pectolinarin at 30 μM were found to significantly induce collagen type I synthesis in OI fibroblasts without an effect on the overall protein synthesis. None of the compounds displayed any toxicity at that concentration. Secretion of collagen into media was not affected by apigenin 7-Oglucuronide and was slightly increased in cells treated with apigenin 7-O-methylglucuronide and pectolinarin. Furthermore, procollagen secreted by treated cells underwent a more rapid processing into collagen as compared with control untreated cells. In addition, we elucidated the possible mechanism involved in their action. Stimulation of collagen biosynthesis was not due to an increase in cell proliferation, because no differences in DNA content between the compound-treated and untreated cells were observed. Since flavonoids are known as strong inhibitors of metalloproteinases degrading matrix proteins, the increased level of collagen could result from the inhibition of their activity. However, the compounds with a stimulatory effect on collagen synthesis did not influence the activities of collagenase type I, gelatinases A and B, and stromelysin. On the contrary, all compounds stimulated the activity of prolidase which catalyzes the final step of collagen degradation and plays an important role in collagen biosynthesis. Stimulation of collagen synthesis and prolidase activity by apigenin 7-Oglucuronide was accompanied by an increase in IGF-I receptor expression. In contrast, the compounds apigenin 7-O-methylglucuronide and pectolinarin which normalized collagen synthesis in OI cells may exert their effects through ß1-integrin-mediated signaling.

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Section: ------------------------------------------------------------supporting

confidence: 64%

Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Stimulation of collagen biosynthesis by flavonoid glycosides in skin fibroblasts of osteogenesis imperfecta type I and the potential mechanism of their action

Gavin¹,

Nazaruk²

2007

Int J Mol Med

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The keloid phenomenon: Progress toward a solution

Louw

2006

Clinical Anatomy

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For centuries, keloids have been an enigma and despite considerable research to unravel this phenomenon no universally accepted treatment protocol currently exists. Historically, the etiology of keloids has been hypothesized by multiple different theories; however, a more contemporary view postulates a multifactoral basis for this disorder involving nutritional, biochemical, immunological, and genetic factors that play a role in this abnormal wound healing. Critical to the process of preventing or managing keloids is the need to locally control fibroblasts and their activities at the wound site. In recent years, considerable evidence has accumulated demonstrating the importance of fatty acids and bioactive lipids in health and disease, especially those involving inflammatory disorders or immune dysfunction. If hypertrophic scarring and keloid formation can be argued to have significant inflammatory histories, then it is possible to postulate a role for lipids in their etiology and potentially in their treatment. This report briefly visits past views and theories on keloid formation and treatment, and offers a theoretical rationale for considering adjuvant fatty acid therapy for keloid management. Sufficient scientific evidence in support of fatty acid strategies for the prevention and treatment of keloids currently exists, which offer opportunities to bridge the gap between the laboratory and the clinic. The intent of this paper is to serve as a basic guideline for researchers, nutritionists, and clinicians interested in keloids and to propose new directions for keloid management.

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Use of dietary phytochemicals to target inflammation, fibrosis, proliferation, and angiogenesis in uterine tissues: Promising options for prevention and treatment of uterine fibroids?

Islam

Akhtar

Ciavattini

et al. 2014

Molecular Nutrition Food Res

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Uterine leiomyomas (fibroids, myomas) are the most common benign tumors of female reproductive tract. They are highly prevalent, with 70–80% of women burdened by the end of their reproductive years. Fibroids are a leading cause of pelvic pain, abnormal vaginal bleeding, pressure on the bladder, miscarriage, and infertility. They are the leading indication for hysterectomy, and costs exceed 6 billion dollars annually in the United States. Unfortunately, no long-term medical treatments are available. Dysregulation of inflammatory processes are thought to be involved in the initiation of leiomyoma and extracellular matrix deposition, cell proliferation, and angiogenesis are the key cellular events implicated in leiomyoma growth. In modern pharmaceutical industries, dietary phytochemicals are used as source of new potential drugs for many kinds of tumors. Dietary phytochemicals may exert therapeutic effects by interfering with key cellular events of the tumorigenesis process. At present, a negligible number of phytochemicals have been tested as therapeutic agents against fibroids. In this context, our aim was to introduce some of the potential dietary phytochemicals that have shown anti-inflammatory, antiproliferative, antifibrotic, and antiangiogenic activities in different biological systems. This review could be useful to stimulate the evaluation of these phytochemicals as possible therapies for uterine fibroids.

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Suppression of insulin-like growth factor signalling pathway and collagen expression in keloid-derived fibroblasts by quercetin: its therapeutic potential use in the treatment and/or prevention of keloids

Cited by 42 publications

References 41 publications

Stimulation of collagen biosynthesis by flavonoid glycosides in skin fibroblasts of osteogenesis imperfecta type I and the potential mechanism of their action

Stimulation of collagen biosynthesis by flavonoid glycosides in skin fibroblasts of osteogenesis imperfecta type I and the potential mechanism of their action

The keloid phenomenon: Progress toward a solution

Use of dietary phytochemicals to target inflammation, fibrosis, proliferation, and angiogenesis in uterine tissues: Promising options for prevention and treatment of uterine fibroids?

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