Suppression of FoxO1 mRNA by β₂‐adrenoceptor–cAMP signaling through miR‐374b‐5p and miR‐7a‐1‐3p in C2C12 myotubes

Abstract

Anti-FoxO1 (#9454) and anti-phospho-FoxO1 (Ser256, #9461) were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH, sc-20357), anti-rabbit IgG (sc-2030), and anti-goat IgG (sc-2020) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Protein extraction and western blot analysisC2C12 myotubes were homogenized in 500 lL of a lysis buffer consisting of 50 mM Tris, 150 mM NaCl, 0.5% 628