Suppression of
            <i>Bamboo Mosaic Virus</i>
            Accumulation by a Putative Methyltransferase in
            <i>Nicotiana benthamiana</i>

Cheng, Ching-Yuan; Hsiao, Y.-Y.; Wu, Hao-Ju; Chuang, C. M.; Chen, Jao-Shien; Tsai, Ching‐Hsiu; Hsu, Yau‐Heiu; Wu, Yi-Chun; Lee, Cheng-Cheng; Meng, Menghsiao

doi:10.1128/jvi.02471-08

Cited by 26 publications

(30 citation statements)

References 38 publications

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“…To search for host factors involved in BaMV replication, each enzymatic domain of the viral replication protein has been used as bait to hunt for its interacting proteins from cDNA libraries prepared from both healthy and BaMV-infected N. benthamiana leaves. A putative plant methyltransferase was found to be associated with the viral RdRp, and it had the potential to suppress the virus replication (5). In this report, we describe another finding: that the viral HLD has a high affinity for the virus's own CP.…”

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confidence: 48%

“…Preparation of N. benthamiana protoplasts and plasmid transfection were performed as described previously (5). In brief, 3 g pCBG, a green fluorescent protein (GFP) gene-carrying BaMV infectious clone, or its derivative was mixed with 1 ϫ 10 5 protoplasts in the presence of final 20% polyethylene glycol 4000.…”

Section: Vol 85 2011 Hld-cp Interaction Is Critical For Bamv Movemementioning

confidence: 99%

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The Interaction between Bamboo Mosaic Virus Replication Protein and Coat Protein Is Critical for Virus Movement in Plant Hosts

Lee

et al. 2011

J Virol

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Bamboo mosaic virus (BaMV) is a positive-sense RNA virus belonging to the genus Potexvirus. Open reading frame 1 (ORF1) encodes the viral replication protein that consists of a capping enzyme domain, a helicase-like domain (HLD), and an RNA-dependent RNA polymerase domain from the N to C terminus. ORF5 encodes the viral coat protein (CP) required for genome encapsidation and the virus movement in plants. In this study, application of a yeast-two hybrid assay detected an interaction between the viral HLD and CP. However, the interaction did not affect the NTPase activity of the HLD. To identify the critical amino acids of CP interacting with the HLD, a random mutational library of CP was created using error-prone PCR, and the mutations adversely affecting the interaction were screened by a bacterial two-hybrid system. As a result, the mutations A209G and N210S in CP were found to weaken the interaction. To determine the significance of the interaction, the mutations were introduced into a BaMV infectious clone, and the mutational effects on viral replication, movement, and genome encapsidation were investigated. There was no effect on accumulations of BaMV CP and genomic RNAs within protoplasts; however, the virus cell-to-cell movement in plants was restricted. Sequence alignment revealed that A209 of BaMV CP is conserved in many potexviruses. Mutation of the corresponding residue in Foxtail mosaic virus CP also reduced the viral HLD-CP interaction and restricted the virus movement, suggesting that interaction between CP and a widely conserved HLD in the potexviral replication protein is crucial for viral trafficking through plasmodesmata.To spread throughout hosts, plant viruses have evolved a number of pathways to allow their progeny to pass across plasmodesmata into neighboring cells and travel along the vascular system (8, 26). The virus-encoded movement proteins play a pivotal role through diverse mechanisms in these cellto-cell and vascular transports. Ancillary proteins, for example, the viral coat proteins (CPs) in some cases, and host factors may also participate in these processes. Numerous studies have been conducted to elucidate the movement mechanisms. Many of the results have been summarized in a number of recent reviews (23,28,30). They provided in-depth discussions on issues such as the identification and characterization of the involved viral and host proteins and the transport models for some exemplified viruses, such as Tobacco mosaic virus (TMV) and Potato virus X (PVX). Despite these efforts, many details of the processes remain elusive.Members of the genus Potexvirus have a positive-strand RNA genome that contains five open reading frames (ORFs), a 5Ј methyl cap, and a 3Ј poly(A) tail. ORF1 encodes the viral replication protein, consisting of a capping enzyme domain, a helicase-like domain (HLD), and an RNA-dependent RNA polymerase domain (RdRp) from the N terminus to the C terminus (16,17). The HLD has RNA 5Ј-triphosphatase and nucleoside triphosphatase (NTPase) activities (18). With the co...

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confidence: 48%

Section: Vol 85 2011 Hld-cp Interaction Is Critical For Bamv Movemementioning

confidence: 99%

The Interaction between Bamboo Mosaic Virus Replication Protein and Coat Protein Is Critical for Virus Movement in Plant Hosts

Lee

et al. 2011

J Virol

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“…With several exceptions (Fujisaki and Ishikawa, 2008; Cheng et al, 2009; Huh et al, 2013), many of the host factors characterized so far in plants positively control viral infection; herein, we refer to them as “positive regulators.” These positive regulators have been characterized predominantly through transient knockdown experiments. Knockdown of a gene encoding a positive regulator of viral infection results in a decrease of viral accumulation.…”

Section: Positive Regulators Of Viral Infection: Genetic Resources Fomentioning

confidence: 99%

Recessive Resistance to Plant Viruses: Potential Resistance Genes Beyond Translation Initiation Factors

et al. 2016

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The ability of plant viruses to propagate their genomes in host cells depends on many host factors. In the absence of an agrochemical that specifically targets plant viral infection cycles, one of the most effective methods for controlling viral diseases in plants is taking advantage of the host plant’s resistance machinery. Recessive resistance is conferred by a recessive gene mutation that encodes a host factor critical for viral infection. It is a branch of the resistance machinery and, as an inherited characteristic, is very durable. Moreover, recessive resistance may be acquired by a deficiency in a negative regulator of plant defense responses, possibly due to the autoactivation of defense signaling. Eukaryotic translation initiation factor (eIF) 4E and eIF4G and their isoforms are the most widely exploited recessive resistance genes in several crop species, and they are effective against a subset of viral species. However, the establishment of efficient, recessive resistance-type antiviral control strategies against a wider range of plant viral diseases requires genetic resources other than eIF4Es. In this review, we focus on recent advances related to antiviral recessive resistance genes evaluated in model plants and several crop species. We also address the roles of next-generation sequencing and genome editing technologies in improving plant genetic resources for recessive resistance-based antiviral breeding in various crop species.

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“…Yeast two-hybrid screen was also used to search for host proteins interacting with the polymerase domain of REP BaMV . An uncharacterized host AdoMet-dependent methyltransferase (PNbMTS1) was thus isolated from the cDNA library prepared from N. benthamiana leaves (Cheng et al, 2009). PNbMTS1 exhibits an AdoMet-dependent inhibitory effect on BaMV CP accumulation in protoplasts.…”

Section: Host Proteins Associated With Repbamvmentioning

confidence: 99%

Function and Structural Organization of the Replication Protein of Bamboo mosaic virus

Meng

Lee

2017

Front. Microbiol.

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The genus Potexvirus is one of the eight genera belonging to the family Alphaflexiviridae according to the Virus Taxonomy 2015 released by International Committee on Taxonomy of Viruses (www.ictvonline.org/index.asp). Currently, the genus contains 35 known species including many agricultural important viruses, e.g., Potato virus X (PVX). Members of this genus are characterized by flexuous, filamentous virions of 13 nm in diameter and 470–580 nm in length. A potexvirus has a monopartite positive-strand RNA genome, encoding five open-reading frames (ORFs), with a cap structure at the 5′ end and a poly(A) tail at the 3′ end. Besides PVX, Bamboo mosaic virus (BaMV) is another potexvirus that has received intensive attention due to the wealth of knowledge on the molecular biology of the virus. In this review, we discuss the enzymatic activities associated with each of the functional domains of the BaMV replication protein, a 155-kDa polypeptide encoded by ORF1. The unique cap formation mechanism, which may be conserved across the alphavirus superfamily, is particularly addressed. The recently identified interactions between the replication protein and the plant host factors are also described.

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Suppression of Bamboo Mosaic Virus Accumulation by a Putative Methyltransferase in Nicotiana benthamiana

Cited by 26 publications

References 38 publications

The Interaction between Bamboo Mosaic Virus Replication Protein and Coat Protein Is Critical for Virus Movement in Plant Hosts

The Interaction between Bamboo Mosaic Virus Replication Protein and Coat Protein Is Critical for Virus Movement in Plant Hosts

Recessive Resistance to Plant Viruses: Potential Resistance Genes Beyond Translation Initiation Factors

Function and Structural Organization of the Replication Protein of Bamboo mosaic virus

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