Suppression of hyphal formation and virulence of<i>Candida albicans</i>by natural and synthetic compounds

Khan, F. Nawaz; Bamunuarachchi, Nilushi Indika; Tabassum, Nazia; Jo, Du-Min; Khan, Mohammad Mansoob; Kim, Young‐Mog

doi:10.1080/08927014.2021.1948538

Cited by 21 publications

(18 citation statements)

References 183 publications

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“…According to Butassi et al [75], the increase in biofilm resistance to antifungals is associated with a significant decrease in total ergosterol content. The filamentous form of C. albicans is considered to be one of the major virulence factors that is responsible for the easier adhesion of cells to surfaces [76]. Figures 3 and 4 demonstrate the morphology of C. albicans cells in the presence of boswellic acids contained in Bioswellix and in the presence of fluconazole (24 h old biofilm).…”

Section: Resultsmentioning

confidence: 99%

Boswellia serrata Extract as an Antibiofilm Agent against Candida spp.

et al. 2022

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The use of antibiotics or antifungals to control infections caused by pathogenic microorganisms is currently insufficiently effective because of their emerging resistance. Thanks to the ability of microorganisms to form a biofilm and thus increase their resistance to administered drugs even more, modern medicine faces the task of finding novel substances to combat infections caused by them. In this regard, the effects of essential oils or plant extracts are often studied. Among the relatively neglected plants is Boswellia serrata, which has a high content of biologically active boswellic acids. In this study, we focused on one of the most common nosocomial infections, which are caused by Candida species. The most common representative is C. albicans, although the number of infections caused by non-albicans species has recently been increasing. We focused on the antifungal activity of Boswellia serrata extract Bioswellix against planktonic and adhering cells of Candida albicans, Candida parapsilosis and Candida krusei. The antifungal activity against adhering cells was further explored by determining the metabolic activity of cells (MTT) and determining the total amount of biofilm using crystal violet. Boswellic acid-containing plant extract was shown to suppress the growth of a suspension population of all tested Candida species. Boswellia serrata extract Bioswellix was most effective in inhibiting C. albicans biofilm formation.

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Section: Resultsmentioning

confidence: 99%

Boswellia serrata Extract as an Antibiofilm Agent against Candida spp.

et al. 2022

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“…Several mechanistic pathways such as an increase in the inhibition of enzyme activity, cell membrane permeability, reduced efflux activity, and depolarization of cell membrane have been attributed to the antimicrobial characteristics of polyphenol solubility. 12 Apart from the native pH of the as-prepared complexes (without any pH adjustment), the antibacterial performances of the same complexes were also studied with pH values of 6.5 and 7.5, which are physiologically relevant. It was observed that pristine histidine did not exhibit lethal toxicity against E. coli up to 10 mM concentration at all the studied pH values.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Edible Acid–Base Complexes of Caffeic Acid with Histidine and Arginine Exhibit Enhanced Antimicrobial and Antioxidant Characteristics

Mude

Thakur

Balapure

et al. 2022

ACS Food Sci. Technol.

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Caffeic acid (CA), an edible plant-derived polyphenol, is known for its various beneficial characteristics such as antimicrobial, antioxidant, and anti-inflammatory. Recently, our group reported a facile acid–base complexation of CA with dopamine (Dopa) to circumvent its poor water solubility. Despite the promising biological characteristics of the CA–Dopa complex, Dopa is permitted only for intravenous but not for oral administration. In the present study, to harness the advantages of CA toward nutraceuticals and food preservatives, we prepared complexes of CA with basic amino acids such as histidine and arginine. The edible as-prepared CA–amino acid complexes have been characterized using FT-IR, UV–visible, and 1H, 13C, and 2D 1H–1H NOESY NMR spectroscopic studies. In addition to the antibacterial activity against Escherichia coli and Staphylococcus aureus, the complexes have also shown high antioxidant characteristics as ascertained by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assay. Further, the in vitro cytotoxicity and superoxide dismutase studies over the mouse fibroblast cells have signified the biocompatibility and radical scavenging performance of the complexes. Finally, the α-amylase assay has shown non-interference of the CA–amino acid complexes in the metabolic function of the enzyme, and thereby proving their potential as edible additives in the pharmaceutic, food, and agro sectors.

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“…Recently, there have also been reports of the possible synergistic effect between a CEO (citronella essential oil) and ZnO NPs (films based on chitosan with zinc oxide, ZnO, andnanoparticles, NPs) [69]. It is also postulated that the combination of dietary flavonoid, quercetin, with fluconazole is effective against C. albicans, including the biofilm created by these strains on the vaginal mucosa in murine Vulvovaginal candidiasis model [70]. However, the mechanism of interaction of the above-mentioned compounds alone and in combination against fungi remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Linear and Disulfide-Cyclized Ultrashort Cationic Lipopeptides Alone and in Combination with Fluconazole against Vulvovaginal Candida spp.

et al. 2021

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Vulvovaginal candidiasis (VVC) occurs in over 75% of women at least once during their lifetime and is an infection that significantly affects their health. Candida strains resistant to standard azole antifungal therapy and relapses of VVC are more and more common. Hypothetically, biofilm is one of the main reasons of relapses and failure of the therapy. Ultrashort cationic lipopeptides (USCLs) exhibit high antimicrobial activities. Our previous study on USCLs revealed that disulfide cyclization can result in selective antifungal compounds. Therefore, four USCL were selected and their antifungal activity were studied on 62 clinical strains isolated from VVC. The results confirmed previous premises that cyclic analogs have increased selectivity between fungal cells and keratinocytes and improved anticandidal activity compared to their linear analogs against both planktonic and biofilm cultures. On the other hand, linear lipopeptides in combination with fluconazole showed a synergistic effect. It was found that the minimum inhibitory concentrations of the tested compounds in combination with fluconazole were at least four times lower than when used separately. Our results indicate that combination therapy of VVC with USCLs and fluconazole at low non-toxic concentrations can be beneficial owing to the synergistic effect. However, further in vivo studies are needed to confirm this hypothesis.

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Suppression of hyphal formation and virulence ofCandida albicansby natural and synthetic compounds

Cited by 21 publications

References 183 publications

Boswellia serrata Extract as an Antibiofilm Agent against Candida spp.

Boswellia serrata Extract as an Antibiofilm Agent against Candida spp.

Edible Acid–Base Complexes of Caffeic Acid with Histidine and Arginine Exhibit Enhanced Antimicrobial and Antioxidant Characteristics

Antifungal Activity of Linear and Disulfide-Cyclized Ultrashort Cationic Lipopeptides Alone and in Combination with Fluconazole against Vulvovaginal Candida spp.

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