Suppression of human cartilage proteoglycan synthesis by rheumatoid synovial fluid mononuclear cells activated with mycobacterial 60‐kd heat‐shock protein

Abstract

Objective. To examine whether T cell reactivity toward heat-shock proteins (HSP) contributes to cartilage destruction in rheumatoid arthritis (RA).Methods. An in vitro system was used, in which human cartilage explants were cocultured with hsp60-activated synovial fluid mononuclear cells (SFMC) from patients with RA, and proteoglycan (PG) synthesis was measured.Results. The hsp60-activated SFMC suppressed cartilage PG synthesis. This effect was dependent on the production of interleukin-1 (IL-1) and tumor necrosis factor a (TNFa).Conclusion. Mycobacterial60-kd heat-shock protein can activate rheumatoid SFMC to suppress human From the

Stimulation of suppressive T cell responses by human but not bacterial 60-kD heat-shock protein in synovial fluid of patients with rheumatoid arthritis.

“…The exact mechanisms by which bacterial hsp60-specific T cells are activated in human but also experimental arthritis are unclear. However, these cells have been demonstrated to have an aggressive phenotype and to induce deleterious effects both in humans and animals (9,36,41,42). By contrast, reactivity of T cells recognizing self hsp60 is accompanied by remission of experimental forms of arthritis and juvenile chronic arthritis (21)(22)(23)(24).…”

Hsp60 chaperonopathies and chaperonotherapy: targets and agents

“…The actions of Hsp60 could lead to the modification of those proteins, and in this manner it could have a role in many diseases, including carcinogenesis. Heart Heart failure; Coronary vascular disease [137][138][139][140] Kidney Glomerulonephritis [141] Large bowel Inflammatory bowel diseases [19,142,143] Lung Chonic obstructive pulmonary disease [20] Oral cavity Periodontitis [144,145] Pancreas Type 1 diabetes [146][147][148][149][150][151] Skin Scleroderma, pemphigoid; Psoriasis; Dermatomyositis [152,153] Synovial joints Rheumatoid arthritis; Juvenile idiopathic arthritis [154][155][156][157] Vessels Vasculitis; Atherosclerosis [158][159][160][161][162][163][164][165][166][167] Adapted from [2]. Hsp60 seems to contribute to tumor cell survival, but this is controversial.…”

Treatment of ankle arthritis with distraction arthroplasty

“…These catabolic cytokines have been implicated in the destruction of cartilage in osteoarthritis. 7 In 2000, van Valburg et al 8 reported the results of joint distraction in a canine model. Osteoarthritis was induced in the knee by transection of the anterior cruciate ligament.…”