Suppression of FPR2 expression inhibits inflammation in preeclampsia by improving the biological functions of trophoblast via NF-κB pathway

Abstract

Purpose The dysfunction of trophoblast during inflammation plays an important role in PE. Formyl peptide receptor 2 (FPR2) plays crucial roles in the development of inflammation-associated disease. This present study aimed to explore the effect of FPR2 on a trophoblast cellular model of preeclampsia. Methods The expression of FPR2 in placenta was detected by immunohistochemical staining and western blotting. Transfection of siRNA was used to knockdown FPR2 in HTR-8/SVneo cells. Inflammatory cytokines were detected by ELISA. CCK8, Transwell, wound healing, FACS and tube formation assays were performed to observe the abilities of cell proliferation, migration, invasion, apoptosis and angiogenesis. Western blotting was implemented to clarify that NF-κB signaling pathway was downstream of FPR2. Results The expression levels of FPR2 were higher in placental tissues of patients with PE. Knockdown of FPR2 expression by siFPR2 or inhibition of its activity by WRW4 decreased the release of proinflammatory cytokines in HTR8/SVneo cells treated with LPS. Knockdown of FPR2 expression or inhibition of its activity further reversed the LPS-induced attenuation of the proliferation, migration, invasion and angiogenesis and increase in apoptosis in HTR8/SVneo cells. Moreover, the NF-κB signaling pathway was activated in both placental tissues of patients with PE and LPS-treated HTR8/SVneo cells. However, the activation was attenuated when FPR2 was knocked down or inhibited. Conclusion Suppression of FPR2 expression alleviated the effects of inflammation induced by LPS on trophoblasts via the NF-κB signaling pathway, which provided a novel and potential strategy for the treatment of PE.

Identification of ferroptosis, necroptosis, and pyroptosis-associated genes in periodontitis-affected human periodontal tissue using integrated bioinformatic analysis

“…For example, Junaid et al found that thrombotic inflammatory disease could be alleviated through the AnxA1/FPR2/ALX pathway Ansari et al (2021). Inhibition of FPR2 expression via the NF-κB signaling pathway attenuates the effects of lipopolysaccharide-induced inflammation on trophoblast cells in pre-eclampsia (Li et al, 2022b). To alleviate chorioamnionitis, Li et al (2020) found that RvD1 attenuated trophoblast inflammation in vivo and in vitro via the FPR2/PPARγ/NF-κB pathway.…”

Pcgf5: An important regulatory factor in early embryonic neural induction

“…The method of immunofluorescence was performed as previously described [ 52 ].Cells were fixed using 4% paraformaldehyde for 20 min at room temperature, permeabilized by 0.3% Triton X-100 for 10 min, blocked with 1xTBS with 3% BSA at room temperature for ∼30 min, incubated with primary antibody overnight at 4 °C, followed by incubation of the appropriate secondary antibody Primary antibodies used in this study were rabbit monoclonal anti-Sox2 (abcam, ab92494; 1/100 in 1xTBS with 3% BSA), rabbit monoclonal anti-Oct4 (abcam, ab181557; 1/100 in 1xTBS with 3% BSA). Secondary antibodies were anti-rabbit IgG labeled with Alexa Fluor 488, 550 (BOSTER, Cat# BA1105 and BA1090; 1/1000 in 1xTBS with 3% BSA).…”

PM2.5 leads to adverse pregnancy outcomes by inducing trophoblast oxidative stress and mitochondrial apoptosis via KLF9/CYP1A1 transcriptional axis

“…Western blotting was applied to detect the protein expression levels in cells or tissues, as described previously ( Li et al, 2022 ). The antibodies used were listed in Supplementary file 4 .…”