Suppression of experimental allergic encephalomyelitis in lewis rats treated with myelin basic protein-liposome complexes: Clinical, histopathological, and cell-mediated immunity correlates

Strejan, Gill H.; Percy, D. H.; Louis, Joanne St.

doi:10.1016/0008-8749(84)90088-1

Cited by 11 publications

(1 citation statement)

References 31 publications

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“…They are known to enhance antibody production to protein antigens [1] and to elicit DH reactions to haptens [10,19], Never theless, only a few experiments on DH reactions to proteins have been reported so far [13,18], Using the hapten-carrier model, we showed here that entrap ment of subimmunogenic doses of antigen led to an tibody-mediated reactions to the hapten, whereas DH reactions to the carrier could be elicited only after ac tivation of macrophages by BCG.…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity of Hapten-Carrier Conjugates Associated with Liposomes

Neveu¹

1987

Int Arch Allergy Immunol

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Immunogenicity of liposome-entrapped hapten-carrier conjugates was studied in guinea pigs. Animals immunized intravenously with a subimmunogenic dose of a hapten-carrier complex entrapped in liposomes exhibited cutaneous anaphylactic reactions to the hapten, even when the liposomes were treated with trypsin, but not delayed hypersensitivity (DH) reactions to the carrier. Animals immunized with a mixture of empty liposomes and antigen showed anaphylactic reactions to the hapten that appeared to be elicited by antigen adsorbed on the outer membrane of liposomes. The induction of anaphylaxis to the hapten by liposome-associated antigen was not due to an adjuvant property of liposomes. DH reactions to the carrier after injection of a liposome-entrapped subimmunogenic dose of a hapten-carrier conjugate could be observed only after activation of macrophages by Bacillus Calmette-Guérin.

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Section: Discussionmentioning

confidence: 99%

Immunogenicity of Hapten-Carrier Conjugates Associated with Liposomes

Neveu¹

1987

Int Arch Allergy Immunol

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Enkephalins and autoimmunity: Differential effect of methionine‐enkephalin on experimental allergic encephalomyelitis in wistar and lewis rats

Janković¹,

Maric²

1987

J of Neuroscience Research

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To evaluate the immunomodulating activity of methionine-enkephalin (Met-Enk) and leucine-enkephalin (Leu-Enk) in the development of experimental allergic encephalomyelitis (EAE), two strains of rats, one highly susceptible to EAE (Lewis rats) and the other relatively resistant to the disease (Wistar rats) were used. The animals were given daily injections of either a high dose (5 mg/kg b.w.) or a low dose (0.2 mg/kg b.w.) of these opioid peptides, after receiving guinea pig spinal cord in combination with immunological adjuvants. A major conclusion from this study is that Met-Enk is a potent immunomodulator/regulator of the autoimmune disease, whereas Leu-Enk does not affect EAE. The high dose of 5 mg/kg b.w. of Met-Enk completely or significantly inhibited neurological signs and markedly diminished occurrence and intensity of histological lesions in the brain, and cervical, thoracic, and lumbar spinal cord. The loss of body weight, which accompanies EAE, was also prevented by a high dose of Met-Enk. In contrast, the low dose of 0.2 mg/kg b.w. of Met-Enk potentiated neurological and histopathological features of the disease. Nonimmunized rats injected with enkephalins for a period of 17 consecutive days showed neither neurological nor histological signs of EAE, nor signs of intoxication due to the application of enkephalins. Thus, these data establish a link between methionine-enkephalin and EAE, and suggest that this opioid pentapeptide might be important in the pathogenesis and prevention of the inflammatory autoimmune disease.

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