Suppression of endotoxin-induced inflammation by taxol

Mirzapoiazova, Tamara; Kolosova, Irina; Moreno, Liliana; Sammani, Saad; Garcia, Joe G.N.; Verin, Alexander D.

doi:10.1183/09031936.00154206

Cited by 86 publications

(70 citation statements)

References 31 publications

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“…This leads to a decreased ability of cells to enter size-limited pores, reflecting the process of neutrophil extravasation, which is the hallmark of RAS. The impairment of neutrophil motility by paclitaxel was also shown in an animal model of pneumonia (42). However, the experimental setup in this study did not allow dissection of the mechanisms of action since paclitaxel also acts on endothelial cells and can block secretion of proteolytic enzymes (43).…”

Section: Discussionmentioning

confidence: 98%

The regulatory role of cell mechanics for migration of differentiating myeloid cells

Lautenschläger

Paschke²,

Schinkinger³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

218

207

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Migration of cells is important for tissue maintenance, immune response, and often altered in disease. While biochemical aspects, including cell adhesion, have been studied in detail, much less is known about the role of the mechanical properties of cells. Previous measurement methods rely on contact with artificial surfaces, which can convolute the results. Here, we used a non-contact, microfluidic optical stretcher to study cell mechanics, isolated from other parameters, in the context of tissue infiltration by acute promyelocytic leukemia (APL) cells, which occurs during differentiation therapy with retinoic acid. Compliance measurements of APL cells reveal a significant softening during differentiation, with the mechanical properties of differentiated cells resembling those of normal neutrophils. To interfere with the migratory ability acquired with the softening, differentiated APL cells were exposed to paclitaxel, which stabilizes microtubules. This treatment does not alter compliance but reduces cell relaxation after cessation of mechanical stress six-fold, congruent with a significant reduction of motility. Our observations imply that the dynamical remodeling of cell shape required for tissue infiltration can be frustrated by stiffening the microtubular system. This link between the cytokeleton, cell mechanics, and motility suggests treatment options for pathologies relying on migration of cells, notably cancer metastasis.acute promyelocytic leukemia ͉ cytoskeleton ͉ microtubules ͉ optical stretcher ͉ paclitaxel

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Section: Discussionmentioning

confidence: 98%

The regulatory role of cell mechanics for migration of differentiating myeloid cells

Lautenschläger

Paschke²,

Schinkinger³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

218

207

View full text Add to dashboard Cite

show abstract

“…Protein concentrations in BAL fluid were measured using an RC DC Protein Assay (Bio-Rad, Hercules, CA), as previously described (16,17). Sample readings (optical density) were converted to mg/ml, using albumin as standard (0.1-1.5 mg/ml).…”

Section: Measurements Of Bal Total Protein Albumin and Concentratiomentioning

confidence: 99%

A Sphingosine 1–Phosphate 1 Receptor Agonist Modulates Brain Death–Induced Neurogenic Pulmonary Injury

Sammani

Park

Zaidi

et al. 2011

Am J Respir Cell Mol Biol

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Lung transplantation remains the only viable therapy for patients with end-stage lung disease. However, the full utilization of this strategy is severely compromised by a lack of donor lung availability. The vast majority of donor lungs available for transplantation are from individuals after brain death (BD). Unfortunately, the early autonomic storm that accompanies BD often results in neurogenic pulmonary edema (NPE), producing varying degrees of lung injury or leading to primary graft dysfunction after transplantation. We demonstrated that sphingosine 1-phosphate (S1P)/analogues, which are major barrier-enhancing agents, reduce vascular permeability via the S1P1 receptor, S1PR1. Because primary lung graft dysfunction is induced by lung vascular endothelial cell barrier dysfunction, we hypothesized that the S1PR1 agonist, SEW-2871, may attenuate NPE when administered to the donor shortly after BD. Significant lung injury was observed after BD, with increases of approximately 60% in bronchoalveolar lavage (BAL) total protein, cell counts, and lung tissue wet/dry (W/D) weight ratios. In contrast, rats receiving SEW-2871 (0.1 mg/kg) 15 minutes after BD and assessed after 4 hours exhibited significant lung protection (z 50% reduction, P ¼ 0.01), as reflected by reduced BAL protein/albumin, cytokines, cellularity, and lung tissue wet/dry weight ratio. Microarray analysis at 4 hours revealed a global impact of both BD and SEW on lung gene expression, with a differential gene expression of enriched immune-response/inflammation pathways across all groups. Overall, SEW served to attenuate the BD-mediated up-regulation of gene expression. Two potential biomarkers, TNF and chemokine CC motif receptor-like 2, exhibited gene array dysregulation. We conclude that SEW-2871 significantly attenuates BD-induced lung injury, and may serve as a potential candidate to improve human donor availability.Keywords: neurogenic pulmonary edema; lung injury; sphingosine 1-phosphate; sphingolipids; lung transplant donors Over the past decade, lung transplantation has become an increasingly important mode of therapy for patients with a variety of endstage lung diseases. The vast majority of lung donors are individuals with brain death (BD). However, the early autonomic storm accompanying brain BD triggers the development of systemic and pulmonary inflammatory responses, which lead to increased pulmonary endothelial permeability (1, 2) and to the sympathetic vasoconstriction of the systemic and pulmonary vasculature. These changes, in addition to possible ischemiareperfusion injury, disrupt the integrity of the alveolar capillary membrane, resulting in neurogenic pulmonary edema (NPE) (1-4). Increased pulmonary interstitial and alveolar fluid accumulation usually develops rapidly after acute injury to the central nervous system (1), and any preventive treatment should be given early after BD (3). This lung injury mimics a form of acute respiratory distress syndrome (ARDS), the most devastating form of acute lung injury (ALI), with excessive ...

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“…Taxol is of particular importance due to its significant anti-cancerigenous activity; its natural accumulation is mainly in the cortex, although it is also stored in the aerial parts in a few species such as T. globosa and T. chinensis. In addition to the highly documented anti-cancer effect of the taxol, it has also been reported that this substance was able to decrease the effect of vascular inflammation in a model of lung injury induced by intratracheal administration of Lipopolysaccharide (LPS) (Mirzapoiazova et al, 2007). Abdominal contortion , and (4) 500 mg Kgary, suggesting a seasonal fluctuation of taxol content in the Mexican yew.…”

Section: Discussionmentioning

confidence: 99%

Taxus globosa Schltdl. (Mexican yew) and Taxus baccata L. (European yew): intra and interspecies analysis of taxol content and biological activity according to different sources

Osuna-Torres

Garcia-Martí²,

Ventura-Zapata

et al. 2015

For. syst.

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Aim of study: Taxus globosa is the only species of Taxus genus that grows in Mexico, and is a tree which is protected by the Mexican Government. This plant shares, with the European species T. baccata, the ability to produce taxanes, such as taxol. The objective of this work was to compare representative samples from two Taxus species: T. globosa Schltdl.("Mexican yew"), and T. baccata L. ("European yew"), by employing different methodological chemical and pharmacological tests.Area of study: Representative samples of Mexican yew were collected from states of Nuevo León, Querétaro, Hidalgo, and Oaxaca. The samples of European yew were taken from a clonal live collection located in the CIEF-Vaersa-Valencia (Spain). Materials and Methods:The aerial parts of all samples were extracted with methanol and subjected to TLC and HPLC analysis. Five extracts were selected for their anti-inflammatory evaluation. The extracts were tested on male ICR mice with ear inflammation induced with TPA, at a dose of 3.2 mg/ear. For T. globosa, an acute toxicity assay in female mice, according to OECD, was also conducted.Main results: Taxol was only detected in the yew trees samples collected in States of Hidalgo and Nuevo León (Mexico). In the inflammatory assay, in all treatments (except for that of MX35) induced slight, but significant, inhibition of inflammation, and only European sample M-9 showed inhibition of edema of >50%. T. globosa in the acute toxicity test causes only mild symptoms associated with a general depression of CNS, without being lethal.Research highlights: The aim of the present study was to perform a chemical analysis through quantification of taxol in the Mexican yew compared to European yew. In this research work was evaluated too for the first time the anti-inflammatory effect from both the Mexican and European yew.Keywords: Acute toxicity; anti-inflammatory activity; European yew; HPLC; Mexican yew. Citation: Osuna-Torres, L., García-Martí, X., Ventura-Zapata, E., López-Upton, J., Zamilpa-Alvarez, A., González-Cortazar, M., Herrera-Ruiz, M., Tapia-Barrera, N. (2015). Taxus

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Suppression of endotoxin-induced inflammation by taxol

Cited by 86 publications

References 31 publications

The regulatory role of cell mechanics for migration of differentiating myeloid cells

The regulatory role of cell mechanics for migration of differentiating myeloid cells

A Sphingosine 1–Phosphate 1 Receptor Agonist Modulates Brain Death–Induced Neurogenic Pulmonary Injury

Taxus globosa Schltdl. (Mexican yew) and Taxus baccata L. (European yew): intra and interspecies analysis of taxol content and biological activity according to different sources

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