Suppression of distinct ovo phenotypes in the Drosophila female germline bymaleless− andSex-lethalM

Oliver, Brian; Pauli, Daniel

doi:10.1002/(sici)1520-6408(1998)23:4<335::aid-dvg8>3.0.co;2-m

Cited by 14 publications

(2 citation statements)

References 59 publications

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“…3D-3D', S5C) and subsequent infertility. This is similar to what is observed in strong alleles of other sex determination genes such as ovo and ovarian tumor (otu), as well as when XY germ cells develop in an XX soma [40,[75][76][77][78][79].…”

Section: Sisterless a Loss-of-function In The Female Germline Results...supporting

confidence: 80%

sisterless Ais required for activation ofSex lethalin theDrosophilagermline

Goyal

Baxter

Doren

2019

Preprint

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In Drosophila, sex determination in somatic cells has been well-studied and is under the control of the switch gene Sex lethal (Sxl), which is activated in females by the presence of two X chromosomes. Though sex determination is regulated differently in the germline versus the soma, Sxl is also necessary and sufficient for the female identity in germ cells. Loss of Sxl function in the germline results in ovarian germline tumors, a characteristic of male germ cells developing in a female soma. Further, XY (male) germ cells expressing Sxl are able to produce eggs when transplanted into XX (female) somatic gonads, demonstrating that Sxl is also sufficient for female sexual identity in the germline. As in the soma, the presence of two X chromosomes is sufficient to activate Sxl in the germline, but the mechanism for "counting" X chromosomes in the germline is thought to be different from the soma. Here we have explored this mechanism at both cis-and trans-levels. Our data support the model that the Sxl "establishment" promoter (SxlPE) is activated in a female-specific manner in the germline, as in the soma, but that the timing of SxlPE activation, and the DNA elements that regulate SxlPE are different from those in the soma. Nevertheless, we find that the X chromosome-encoded gene sisterless A (sisA), which helps activate Sxl in the soma, is also essential for Sxl activation in the germline. Loss of sisA function leads to loss of Sxl expression in the germline, and to ovarian tumors and germline loss. These defects can be rescued by the expression of Sxl, demonstrating that sisA lies upstream of Sxl in germline sex determination. We conclude that sisA acts as an X chromosome counting element in both the soma and the germline, but that additional factors that ensure robust, female-specific expression of Sxl in the germline remain to be discovered.

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Section: Sisterless a Loss-of-function In The Female Germline Results...supporting

confidence: 80%

sisterless Ais required for activation ofSex lethalin theDrosophilagermline

Goyal

Baxter

Doren

2019

Preprint

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“…The molecular function of OTU is unknown, but it may involve RNA regulation (Goodrich et al, 2004). Finally, Sxl acts genetically downstream of ovo and otu, and these genes are required for the proper splicing of Sxl RNA into the female (active) form (Bopp et al, 1993;Nagoshi et al, 1995;Oliver et al, 1993;Oliver and Pauli, 1998;Pauli et al, 1993).…”

Section: Germ Cell-autonomous Controlmentioning

confidence: 99%

The control of sexual identity in theDrosophilagermline

Casper

Doren

2006

Development

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DEVELOPMENT 2783Whether to be male or female is a critical decision in development. Nowhere is this more important than in the germ cells, which must produce either the sperm or eggs necessary for the perpetuation of the species. How does a germ cell make this decision and how is it executed? One thing that is clear is that this process is very different in germ cells compared with other cells of the embryo. Here, we explore how sexual identity is established in the Drosophila germline, how this affects other aspects of germ cell development and what studies in Drosophila can teach us about mammalian germ cells.

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