Suppression of calphobindin I (CPB I) production in carcinoma of uterine cervix and endometrium

Karube, Akihiro

doi:10.1016/0020-7292(96)80408-5

Cited by 10 publications

(13 citation statements)

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“…The presence of spots containing Annexin A1, A5 and A6 was significantly decreased in the UM-A cell line, as described previously in various oncogenesis processes, including skin melanoma. [27][28][29] The differentially expressed proteins Rho GDPI 1 and 2, mostly present in the UM primary cell culture, also correlated well with previous reports involving the downregulation of those proteins in late stages of cancer. 30,31 On the other hand, we found proteins previously linked to tumour development or related to cell migration and invasion that were upregulated or exclusively present in the primary cell culture.…”

Section: Differential Proteome Analysis Of Human Uveal Melanoma Primasupporting

confidence: 75%

The characterization of the invasion phenotype of uveal melanoma tumour cells shows the presence of MUC18 and HMG‐1 metastasis markers and leads to the identification of DJ‐1 as a potential serum biomarker

Pardo¹,

García²,

Thomas³

et al. 2006

Intl Journal of Cancer

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Uveal malignant melanoma (UM) is the most frequent primary intraocular tumour in adult humans. Because the survival rate of patients with UM has changed little in the past few decades, a better understanding of the molecular events governing UM development and the identification of markers indicating the potential for metastasis at the time of diagnosis are necessary to design improved and more specific treatments. In this study, we investigated UM tumour development by comparing two recently established UM cultures with different invasion potential by twodimensional gel electrophoresis. Protein features expressed differentially were identified by mass spectrometric analysis. Potential markers were assayed in both cultures and in long-term established UM cell lines (UW-1, OCM-1, SP6.5 and 92.1) by Western blotting and their role in invasion analysed using Matrigel membranes. Comparative analysis revealed that UM cultures with lowand high-grade invasion potential differ in their cellular metabolism and, more interestingly, in several cancer-associated proteins, including those implicated in cell adhesion and migration, proliferation and various oncogenes. Our data indicate a correlation between MUC18 and HMG-1 expression and the invasiveness of UM cells. We also demonstrate the expression and secretion of DJ-1 oncoprotein by UM cells. We suggest a possible role for MUC18 and HMG-1 proteins in UM cell invasion. The secretion of DJ-1 by UM cells, and the ability to detect this protein in UM patients' sera implicate it as a potential noninvasive biomarker for this malignancy. ' 2006 Wiley-Liss, Inc.Key words: uveal melanoma; invasion; proteomics; MUC-18; DJ-1 Uveal malignant melanoma (UM) is the most frequent intraocular tumour in adult humans.1 Unlike cutaneous melanoma, uveal melanoma disseminates mainly through the blood stream and preferentially establishes metastases in the liver. Metastatic liver disease is the leading cause of death in uveal melanoma and can develop after a long disease-free interval, which suggests the presence of occult micrometastatic disease at the time the primary eye tumour is diagnosed and treated.2 Unfortunately, advances in eye cancer treatment have not paralleled those made in the management of other types of cancer, and the survival rate of patients with uveal melanoma has changed little in the past few decades.3 A better understanding of the molecular events governing uveal melanoma development and the identification of markers indicating the potential for metastasis at the time of diagnosis are necessary to design improved and more specific treatments. Various clinical and molecular prognostic factors have been suggested in uveal melanoma, but none has proved to be sufficiently useful or viable for routine clinical use. 4,5 Currently, there are many challenging technologies and approaches to identify tumour markers for prognostic and therapeutic purposes.6 Transcriptional studies alone are not sufficient, with several investigators reporting a poor correlation between mRNA and p...

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Section: Differential Proteome Analysis Of Human Uveal Melanoma Primasupporting

confidence: 75%

The characterization of the invasion phenotype of uveal melanoma tumour cells shows the presence of MUC18 and HMG‐1 metastasis markers and leads to the identification of DJ‐1 as a potential serum biomarker

Pardo¹,

García²,

Thomas³

et al. 2006

Intl Journal of Cancer

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“…In general, annexins have been implicated in the maintenance of calcium homeostasis, signal transduction, receptor-mediated endocytosis, cell proliferation and growth, ion channel formation, and the secretion of neurotransmitters and hormones (34,35). AxV, in particular, has been implicated in regulating cell proliferation (36) and as a protein kinase C inhibitor (37,38).…”

Section: Discussionmentioning

confidence: 99%

Annexin V Associates with the IFN-γ Receptor and Regulates IFN-γ Signaling

León

Nandan

López

et al. 2006

The Journal of Immunology

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Many of the biological activities of IFN-γ are mediated through the IFN-γR3-linked Jak-Stat1α pathway. However, regulation of IFN-γ signaling is not fully understood, and not all responses to IFN-γ are Stat1α dependent. To identify novel elements involved in IFN-γ cell regulation, the cytoplasmic domain of the R2 subunit of the human IFN-γR was used as bait in a yeast two-hybrid screen of a human monocyte cDNA library. This identified annexin A5 (AxV) as a putative IFN-γR binding protein. The interaction was confirmed in pull-down experiments in which a GST-R2 cytoplasmic domain fusion protein was incubated with macrophage lysates. Furthermore, immunoprecipitation using anti-IFN-γR2 Abs showed that AxV interacted with IFN-γR2 to form a stable complex following incubation of cells with IFN-γ. In 293T cells with reduced expression of AxV, brought about by small interfering RNA targeting, activation of Jak2 and Stat1α in response to IFN-γ was enhanced. Inhibition of cell proliferation, a hallmark of the IFN-γ response, also was potentiated in HeLa cells treated with small interfering RNA directed at AxV. Taken together, these results suggest that through an inducible association with the R2 subunit of the IFN-γR, AxV modulates cellular responses to IFN-γ by modulating signaling through the Jak-Stat1 pathway.

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“…For example, the loss of annexin A5 was identified as a marker for cutaneous squamous cell carcinoma (41), whereas annexin A5 protein expression was augmented in growth hormone-secreting carcinoma (42). Importantly the expression of annexin A5 was markedly suppressed in both cervical and endometrial carcinoma cells when compared with their normal counterparts (43). Because the HeLa cell line used in the present study is a type of human cervical carcinoma cell line, the increased expression of annexin A5 in GAD-treated HeLa cells may contribute to growth inhibition induced by GAD.…”

Section: Figmentioning

confidence: 94%

Proteomics Characterization of the Cytotoxicity Mechanism of Ganoderic Acid D and Computer-automated Estimation of the Possible Drug Target Network

Yue

Cao

Guan

et al. 2008

Molecular & Cellular Proteomics

176

112

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Suppression of calphobindin I (CPB I) production in carcinoma of uterine cervix and endometrium

Cited by 10 publications

References 0 publications

The characterization of the invasion phenotype of uveal melanoma tumour cells shows the presence of MUC18 and HMG‐1 metastasis markers and leads to the identification of DJ‐1 as a potential serum biomarker

The characterization of the invasion phenotype of uveal melanoma tumour cells shows the presence of MUC18 and HMG‐1 metastasis markers and leads to the identification of DJ‐1 as a potential serum biomarker

Annexin V Associates with the IFN-γ Receptor and Regulates IFN-γ Signaling

Proteomics Characterization of the Cytotoxicity Mechanism of Ganoderic Acid D and Computer-automated Estimation of the Possible Drug Target Network

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