Suppression of bank vole pancreatic islet function by proinflammatory cytokines

Blixt, Martin; Niklasson, Bo; Sandler, Stellan

doi:10.1016/j.mce.2009.02.010

Cited by 5 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In comparison to human islets, the bank vole islets display a similar response in stimulation index and in insulin content with increasing glucose concentration in the culture medium (Eizirik et al 1992b). Interestingly, we recently found that bank vole islets responded to proinflammatory cytokines in vitro according to a pattern more resembling human islets than that seen in rodent islets (Blixt et al 2009). …”

Section: Discussionmentioning

confidence: 87%

Pancreatic islets of bank vole show signs of dysfunction after prolonged exposure to high glucose concentrations in vitro

Blixt

Niklasson²,

Sandler³

2010

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

Bank voles develop glucose intolerance/diabetes mellitus when kept in captivity. We have characterized b-cell function of glucose intolerant/diabetic animals, and found that this animal model has features of both human type 1 and type 2 diabetes. The aim of this study was to study the functional alterations of islets isolated from glucose tolerant bank voles after a prolonged exposure to various glucose concentrations in vitro. For this purpose, pancreatic islets from normal (glucose tolerant) male and female bank voles were cultured at different glucose concentrations (5 . 6, 11 . 1 (control), or 28 mM) whereupon islet functions were examined. Overall, islet insulin output was lowered at 5 . 6 mM glucose, and similar to control, or enhanced after culture in 28 mM glucose. High glucose culture led to decreased insulin contents, but there was no change in islet DNA content and in morphological assessments of cell death, with the latter findings suggesting that the so-called glucotoxicity had not evolved. A slight gender difference was observed in that islets isolated from females exhibited a glucose-regulated (pro)insulin biosynthesis rate and insulin gene expression. In conclusion, we have found that islets isolated from female and male bank voles are affected by glucose concentrations in vitro in that some signs of dysfunction were observed upon high glucose exposure. A minor gender difference was observed suggesting that the islets of the females may more readily adapt to the elevated glucose concentration than islets of the male bank voles. It could be that these in vitro gender differences observed may represent a mechanism underlying the gender difference in diabetes development observed among bank voles.

show abstract

Section: Discussionmentioning

confidence: 87%

Pancreatic islets of bank vole show signs of dysfunction after prolonged exposure to high glucose concentrations in vitro

Blixt

Niklasson²,

Sandler³

2010

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The second phase of pulsatile insulin secretion is related to the islet insulin content [ 19 ]. Hence, the insulin content in the medium of isolated islet is consisting of insulin secretion and amount or formation of insulin [ 20 , 21 ]. Therefore, present results demonstrated that low dose and high dose of eugenol administration were more potent on insulin content of pancreatic islets in hypo- and hyperglycemic conditions, and it can be suggested that these doses of eugenol have more impact on the second phase of insulin release via mobilizing of subsequent supply for producing new granules that contain insulin.…”

Section: Discussionmentioning

confidence: 99%

Eugenol Improves Insulin Secretion and Content of Pancreatic Islets from Male Mouse

Oroojan

2020

International Journal of Endocrinology

View full text Add to dashboard Cite

Glucose homeostasis is required for control of insulin secretion. Phenolic compounds improved glucose-stimulated insulin secretion (GSIS). Eugenol is a phenolic compound that may increase GSIS. So, it was decided to investigate the effect of eugenol on the insulin secretion and content of pancreatic islets from the male mice. In this experimental study, 3-month-old NMRI mice (20–25 g) were prepared. The pancreatic islets of Langerhans were isolated using the collagenase digestion method and divided into 12 groups: glucose 2.8, 5.6, and 16.7 mM, glucose 2.8 mM + eugenol 50, 100, and 200 µM, glucose 5.6 mM + eugenol 50, 100, and 200 µM, and glucose 16.7 mM + eugenol 50, 100, and 200 µM. The islet’s insulin secretion and content were measured after 1 hour and 24 hours incubation at 37°C, respectively, by the ELISA assays method and related commercial kit. Present results showed that all doses of eugenol increased islet’s insulin secretion and content in the medium containing glucose concentrations 2.8, 5.6, and 16.7 mM (P < 0.05). In conclusion, eugenol as a phenolic compound increased insulin secretion and content of pancreatic islets. The moderate dose of this compound enhanced insulin secretion during hypo- and hyperglycemic conditions, as well as a high dose of eugenol, increased insulin content. Finally, present research suggested that the administration of eugenol 100 µM was suitable for the early stage of T2DM as well as eugenol 200 µM for the advanced stage of this disease.

show abstract

“…Post-transplantation apoptosis in human islets can be attributed by inflammatory factors. In the early stages of transplantation, human islet function is suppressed by pro-inflammatory cytokines [ 10 , 11 ], which can stimulate the c-JNK islet cell apoptosis pathway. Upon activation of the apoptosis pathway, human islet cells could consequently release more pro-inflammatory cytokines which could attract and activate leukocytes.…”

Section: Introductionmentioning

confidence: 99%

Anti-apoptotic Effects of Bone Marrow on Human Islets: A Preliminary Report

Luo

2015

J Stem Cell Res Ther

View full text Add to dashboard Cite

Apoptosis is one of the major factors contributing to the failure of human islet transplantation. Contributors to islet apoptosis exist in both the pre-transplantation and post transplantation stages. Factors include the islet isolation process, deterioration in vitro prior to transplantation, and immune rejection post transplantation. Previous studies have demonstrated that co-cultured bone marrow cells with human islets not only significantly enhanced the longevity of human islets but also maintained function. We hypothesized that the protective effects of bone marrow cells on human islets are through mechanisms related to preventing apoptosis. This study observed the levels of inflammatory factors such as interleukin-1β (IL-1β), the release of extracellular ATP in vitro, and expression levels of P2X7 ATP receptor (P2X7R), all of which lead to the occurrence of apoptosis in human islets. When human islets were co-cultured with human bone marrow, there was a reduction in the rate of apoptosis correlated with the reduction in inflammatory factors, extra cellular ATP accumulation, and ATP receptor P2X7R expression versus human islets cultured alone. These results suggest that co-culturing bone marrow cells with human islets inhibits inflammation and reduces apoptosis, thus protecting islets from self-deterioration.

show abstract

Suppression of bank vole pancreatic islet function by proinflammatory cytokines

Cited by 5 publications

References 33 publications

Pancreatic islets of bank vole show signs of dysfunction after prolonged exposure to high glucose concentrations in vitro

Pancreatic islets of bank vole show signs of dysfunction after prolonged exposure to high glucose concentrations in vitro

Eugenol Improves Insulin Secretion and Content of Pancreatic Islets from Male Mouse

Anti-apoptotic Effects of Bone Marrow on Human Islets: A Preliminary Report

Contact Info

Product

Resources

About