Suppression of argonautes compromises viral infection in Penaeus monodon

Ho, Teerapong; Panyim, Sakol; Udomkit, Apinunt

doi:10.1016/j.dci.2018.09.008

Cited by 3 publications

(4 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous research has reported that a range of molecules including natural compounds, recombinant proteins, virus-like particles (VLPs), DNA vaccines, and specific double-stranded RNAs could all suppress viral replication or prevent viral infection in shrimp [13,36]. Amongst these, the application of specific double-stranded RNAs is a powerful strategy to suppress endogenous genes [10,37,38] or viral gene expression [14,15,33,39] to significantly inhibit viral replication in vitro and in vivo in shrimp. Recently, the co-expression of PstDNV capsid and dsRNA-YHV-pro was applied to protect shrimp from YHV infection [32].…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Production of a Virus-Like Particle Linked to dsRNA to Enhance dsRNA Delivery for Yellow Head Virus Inhibition

Worawittayatada

Angsujinda

Sinnuengnong

et al. 2022

Viruses

View full text Add to dashboard Cite

A co-expressed Penaeus stylirostris densovirus (PstDNV) capsid and dsRNA specific to the yellow head virus (YHV) protease (CoEx cpPstDNV/dspro) has been shown to suppress YHV replication in the Pacific white-legged shrimp (Litopenaeus vannamei). However, maintaining two plasmids in a single bacterial cell is not desirable; therefore, a single plasmid harboring both the PstDNV capsid and the dsRNA-YHV-pro gene was constructed under the regulation of a single T7 promoter, designated pET28a-Linked cpPstDNV-dspro. Following induction, this novel construct expressed an approximately 37-kDa recombinant protein associated with a roughly 400-bp dsRNA (Linked cpPstDNV-dspro). Under a transmission electron microscope, the virus-like particles (VLP; Linked PstDNV VLPs-dspro) obtained were seen to be monodispersed, similar to the native PstDNV virion. A nuclease digestion assay indicated dsRNA molecules were both encapsulated and present outside the Linked PstDNV VLPs-dspro. In addition, the amount of dsRNA produced from this strategy was higher than that obtained with a co-expression strategy. In a YHV infection challenge, the Linked PstDNV VLPs-dspro was more effective in delaying and reducing mortality than other constructs tested. Lastly, the linked construct provides protection for the dsRNA cargo from nucleolytic enzymes present in the shrimp hemolymph. This is the first report of a VLP carrying virus-inhibiting dsRNA that could be produced without disassembly and reassembly to control virus infection in shrimp.

show abstract

Section: Discussionmentioning

confidence: 99%

Simultaneous Production of a Virus-Like Particle Linked to dsRNA to Enhance dsRNA Delivery for Yellow Head Virus Inhibition

Worawittayatada

Angsujinda

Sinnuengnong

et al. 2022

Viruses

View full text Add to dashboard Cite

show abstract

“…12 In contrast, in invertebrates, such as shrimps, there are more than one Dicer gene with different functional activities in RNAi machinery. 15−17 Likewise, Ago1 and Ago3 genes are involved in dsRNA-mediated gene silencing pathway in shrimps; 18 and Ago4 showed irrelevant association with RNAi-mediated gene silencing and defense against viral infection. 19 In this context, RNAi machinery seems to primarily mediate gene regulation through miRNAs in mammals.…”

Section: ■ Introductionmentioning

confidence: 99%

“…22 Dicer and Ago are significantly associated with and preferentially required for viral infections in aquatic invertebrates, such as giant tiger prawn (Penaeus monodon) and Pacific oysters (Crassostrea gigas). 18,23 However, RNAi machinery might also target chromatin remodeling, for example, which induces genomic methylation. 7,24 RNAi can be used as a research tool to mediate gene silencing for studying the function of genes through reverse genetics (Table 1).…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, RNAi machinery is functional and seemed to be a backline defense in case of any defect in the IFN system in vertebrates. , In addition, mammals have a single Dicer that participates in the generation of both siRNAs and miRNAs and have four Ago genes that are responsible for microRNAs (miRNAs)-guided gene silencing, and Ago2 only mediates siRNA-dependent RNAi . In contrast, in invertebrates, such as shrimps, there are more than one Dicer gene with different functional activities in RNAi machinery. − Likewise, Ago1 and Ago3 genes are involved in dsRNA-mediated gene silencing pathway in shrimps; and Ago4 showed irrelevant association with RNAi-mediated gene silencing and defense against viral infection . In this context, RNAi machinery seems to primarily mediate gene regulation through miRNAs in mammals …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

RNA Interference in Aquaculture: A Small Tool for Big Potential

Abo‐Al‐Ela

2021

J. Agric. Food Chem.

View full text Add to dashboard Cite

For decades, the tight regulatory functions of DNA and RNA have been the focus of extensive research with the goal of harnessing RNA molecules (e.g., microRNA and small interfering RNA) to control gene expression and to study biological functions. RNA interference (RNAi) has shown evidence of mediating gene expression, has been utilized to study functional genomics, and recently has potential in therapeutic agents. RNAi is a natural mechanism and a well-studied tool that can be used to silence specific genes. This method is also used in aquaculture as a research tool and to enhance immune responses. RNAi methods do have their limitations (e.g., immune triggering); efficient and easy-to-use RNAi methods for large-scale applications need further development. Despite these limitations, RNAi methods have been successfully used in aquaculture, in particular shrimp. This review discusses the uses of RNAi in aquaculture, such as immune- and production-related issues and the possible limitations that may hinder the application of RNAi in the aquaculture industry. Our challenge is to develop a highly potent in vivo RNAi delivery platform that could complete the desired action with minimal side effects and which can be applied on a large-scale with relatively little expense in the aquaculture industry.

show abstract

Assessment of the function of gonad-specific PmAgo4 in viral replication and spermatogenesis in Penaeus monodon

Panyim

Udomkit

2021

Developmental & Comparative Immunology

View full text Add to dashboard Cite

Suppression of argonautes compromises viral infection in Penaeus monodon

Cited by 3 publications

References 67 publications

Simultaneous Production of a Virus-Like Particle Linked to dsRNA to Enhance dsRNA Delivery for Yellow Head Virus Inhibition

Simultaneous Production of a Virus-Like Particle Linked to dsRNA to Enhance dsRNA Delivery for Yellow Head Virus Inhibition

RNA Interference in Aquaculture: A Small Tool for Big Potential

Assessment of the function of gonad-specific PmAgo4 in viral replication and spermatogenesis in Penaeus monodon

Contact Info

Product

Resources

About