Annual bluegrass (ABG) (Poa annua L.) is a prolific seed producer in the spring on golf courses that in turn decreases aesthetic quality and trueness of ball roll on coolseason putting greens. Proxy (ethephon) applied twice in the spring after green-up is the current industry standard after the loss of Embark (mefluidide) from the turf and ornamental market. However, plant growth regulators including Proxy have been used for years to help suppress ABG seedheads with inconsistent success. The primary objective of this study was to determine if ABG seedhead suppression is improved by adding a late fall application of Proxy to the two traditional spring applications of plant growth regulators at nine locations with diverse environments. A second objective was to determine the importance of including Primo Maxx (trinexapac-ethyl) in fall and spring applications. Adding a late fall application of Proxy prior to the two spring applications (F+S+S) improved control of ABG seedheads over the traditional two spring applications (S+S), but the magnitude of improvement varied among locations. Abbreviations: ABG, annual bluegrass; AUSCPC, area under seedhead cover progress curve; CBG, creeping bentgrass; DAISA, days after initial spring application; F+S+S, Proxy + Primo Maxx applied once in fall plus twice in spring; GDD, growing degree day; S+S, Proxy + Primo Maxx applied twice in spring. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.