Suppression of anchorage-independent growth and matrigel invasion and delayed tumor formation by elevated expression of fibulin-1D in human fibrosarcoma-derived cell lines

Jiang, Qing; Maher, Veronica M.; Tran, Hoanh; Argraves, W. Scott; Dunstan, R W; McCormick, J. Justin

doi:10.1038/sj.onc.1201385

Cited by 91 publications

(84 citation statements)

References 19 publications

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“…Our results showed that ®bulin-1C was the predominant variant form expressed in ovarian cancer cells and that the ®bulin-1C:1D mRNA ratio was higher in ovarian cancers as compared to normal ovaries. This is consistent with the anti-oncogenic properties of the 1D form reported by Qing et al (1997). In their study, increased expression of ®bulin-1D in human ®brosar-coma-derived cell lines was shown to suppress anchorage-independent growth and matrigel invasion, while delaying the onset of tumor formation in athymic mice (Qing et al, 1997).…”

Section: Discussionsupporting

confidence: 77%

“…This is consistent with the anti-oncogenic properties of the 1D form reported by Qing et al (1997). In their study, increased expression of ®bulin-1D in human ®brosar-coma-derived cell lines was shown to suppress anchorage-independent growth and matrigel invasion, while delaying the onset of tumor formation in athymic mice (Qing et al, 1997). Imbalanced expression of ®bulin-1 variants in ovarian cancer cells may thus re¯ect an increased aggressive phenotype of malignant cells.…”

Section: Discussionsupporting

confidence: 76%

“…Moreover, the biological function of ®bulin-1 appears to be variant-speci®c. On the one hand, ®bulin-1D has been shown to have anti-oncogenic properties after expression in transfected cancer cells, by decreasing the invasive phenotype and metastatic potential in nude mice (Qing et al, 1997). On the other hand, ®bulin-1C was recently found to bind NOVH, a connective tissue growth factor, through its C-terminal region (Perbal et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Estrogen induction and overexpression of fibulin-1C mRNA in ovarian cancer cells

Katsaros²,

et al. 2002

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Fibulin-1 is an extracellular matrix protein induced by estradiol in estrogen receptor (ER) positive ovarian cancer cell lines. Alternative splicing of ®bulin-1 mRNA results in four di erent variants named A, B, C and D that may have distinct biological functions. We studied the relative expression of ®bulin-1 mRNA variants and their estrogen regulation in human ovarian cancer cells. In ovarian tissues and cancer cell lines, ®bulin-1C and -1D are the predominant forms, whereas ®bulin-1A and -1B are weakly expressed. We developed a competitive PCR assay based on coampli®cation of ®bulin-1C and -1D to study the relative expression of these ®bulin-1 variants in human ovarian samples. In ovarian cancer cell lines and ovarian cancer samples, there was a marked increase in the ®bulin-1C:1D and ®bulin-1C:HPRT mRNA ratios as compared to normal ovaries. In the BG1 estrogen receptor positive ovarian cancer cell line, ®bulin-1C mRNA was induced by estradiol in a dose-and time-dependent manner. Since others and we have previously shown an increased expression of ERa as compared to ERb in ovarian cancer cells, we investigated whether ERa or ERb is involved in this induction. For this aim, MDA-MB-231 breast cancer cell line, which expresses both low basal levels of ERs and ®bulin-1, was infected with recombinant ERa or ERb encoding adenovirus and treated with estradiol. Fibulin-1C was induced by estradiol in ERa-but not ERb-infected cells, suggesting that ®bulin-1C induction is mediated through ERa. In ovarian tumors, a trend towards a correlation between ®bulin-1C and ERa expression levels was noted. In conclusion, this study showed an increased ®bulin-1C: -1D mRNA ratio in ovarian cancer cells as compared to normal ovaries. This ®nding suggests that the C variant may be involved in ovarian carcinogenesis. Fibulin-1C overexpression may thus be a clue for the understanding of a putative role of estrogens in ERa promoted ovarian tumor progression.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

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Estrogen induction and overexpression of fibulin-1C mRNA in ovarian cancer cells

Katsaros²,

et al. 2002

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“…Fbln-1 inhibits in vitro adhesion and motility of various carcinoma cell lines (Twal et al, 2001). Fbln-1 also suppresses the growth of human fibrosarcomas in nude mice (Qing et al, 1997) and human papillomavirus E6-mediated transformation (Du et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have implicated Fbln-1 as having a role in cancer (Qing et al, 1997;Twal et al, 2001;Du et al, 2002;Moll et al, 2002;Greene et al, 2003). An elevated expression of Fbln-1 is associated with human breast tumours (Forti et al, 2002;Greene et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Immunological and pathobiological roles of fibulin-1 in breast cancer

et al. 2003

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Fibulin-1 (Fbln-1) is an immunogenic breast cancerrelated glycoprotein identified by serological analysis of cDNA expression library (SEREX) strategy. Here, we show that dendritic cells from two breast cancer patients elicited a CD4 þ -mediated T-cell response to Fbln-1 presentation. In both patients, an antibody response to Fbln-1 was also found. By contrast, a Fbln-1-seronegative patient and a weakly seropositive patient demonstrated no such T-cell response. Analysis of human breast cancers for Fbln-1 RNA and protein expression revealed the presence of Fbln-1C and -1D variants. Fbln-1 was detected in the cytoplasm and at the cell surface of different human breast carcinoma cell lines. Immunohistochemical analysis of 528 archival primary breast carcinomas showed the expression of Fbln-1 in 35% of the cases. When the immunohistochemical findings were compared against pathobiological information associated with each specimen, an inverse relationship between Fbln-1 and cathepsin D expression was observed (P ¼ 0.04). Furthermore, even though long-term survival was similar between Fbln-1-positive and -negative cases, the survival of Fbln-1-positive cases improved when a lymphoid infiltrate was present at the tumour site. Taken together, our findings of an Fbln-1-specific immunity and the improved survival associated with Fbln-1 expression in the presence of lymphoid infiltration point to a role of Fbln-1 in tumour immunosurveillance.

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Molecular events associated with dysplastic morphologic transformation and initiation of ovarian tumorigenicity

Yang

Smith

Cohen

et al. 2002

Cancer

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The loss of Dab2 is closely associated with the transition of ovarian surface epithelial cells to premalignant states and is likely involved in the initiation of ovarian tumorigenicity. Transient loss of collagen IV and laminin in the basement membrane of the premalignant epithelium and subsequent inactivation of Dab2 are common early events associated with tumorigenicity of the ovarian surface epithelium.

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Suppression of anchorage-independent growth and matrigel invasion and delayed tumor formation by elevated expression of fibulin-1D in human fibrosarcoma-derived cell lines

Cited by 91 publications

References 19 publications

Estrogen induction and overexpression of fibulin-1C mRNA in ovarian cancer cells

Estrogen induction and overexpression of fibulin-1C mRNA in ovarian cancer cells

Immunological and pathobiological roles of fibulin-1 in breast cancer

Molecular events associated with dysplastic morphologic transformation and initiation of ovarian tumorigenicity

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