Suppression of acute experimental allergic encephalomyelitis by intraperitoneal administration of synaptosomal antigens

Degano, Alicia L.; Bucher, A.E.; Roth, German A.

doi:10.1002/(sici)1097-4547(19980715)53:2<187::aid-jnr7>3.0.co;2-3

Cited by 8 publications

(14 citation statements)

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“…22 Briefly, 1.25 × 10 6 cells per ml were cultured in a total volume of 200 µl per well of cRPMI with 0.5 µg ml −1 concanavalin A (Con A) in the presence or absence of ABL (0.25-1 µg ml −1 ) for 4 days at 37°C, 5% CO 2 . 22 Briefly, 1.25 × 10 6 cells per ml were cultured in a total volume of 200 µl per well of cRPMI with 0.5 µg ml −1 concanavalin A (Con A) in the presence or absence of ABL (0.25-1 µg ml −1 ) for 4 days at 37°C, 5% CO 2 .…”

Section: Proliferation Measurement Of Mononuclear Cells (Mnc)mentioning

confidence: 99%

In vivo immunomodulatory effect of the lectin from edible mushroom Agaricus bisporus

et al. 2016

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Lectins are glycan-binding proteins that are resistant to digestion in the gastrointestinal tract and enter intact to blood circulation. The aim of this study was to evaluate the influence of edible mushroom Agaricus bisporus lectin (ABL) on innate and adaptive immune responses as well as its effect in two different experimental pathologies that involve the immune system. ABL inhibited in vitro nitric oxide (NO) production by mouse peritoneal macrophages in response to the pro-inflammatory stimuli lipopolysaccharides (LPS). However, it did not modify the activity of arginase, showing that while ABL downregulates M1 activation, it does not affect M2 activation. ABL also inhibited mononuclear cell proliferation in response to mitogen Con A, or in a mixed lymphocyte reaction. During the in vivo studies, oral administration of ABL to BALB/c mice induced a marked inhibition of NO production by peritoneal macrophages after LPS stimuli. The influence of ABL on tumor growth was studied in BALB/c mice receiving daily oral doses of ABL and implanted with CT26 tumor cells. ABL treatment induced significantly higher rate of tumor growth when compared with control mice. On the other hand, oral ABL administration in Wistar rats induced a marked diminution of the incidence of the disease and the severity of the clinical signs of experimental autoimmune encephalomyelitis. We can conclude that ABL has an in vivo immunomodulatory effect reducing the innate and adaptive responses. This food lectin shows potential therapeutic application on control of inflammatory autoimmune pathologies.

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Section: Proliferation Measurement Of Mononuclear Cells (Mnc)mentioning

confidence: 99%

In vivo immunomodulatory effect of the lectin from edible mushroom Agaricus bisporus

et al. 2016

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“…The clinical analysis of the different groups of animals sensitized to induce EAE is summarized in Table I. As shown previously (Degano et al, 1998; Ditamo et al, 2005), about 80% of animals of the control group (treated with vehicle alone) showed the characteristic signs of the disease. Despite this fact, the rest of the animals manifested a subclinical state of disease, with reactivity to MBP and histological alterations.…”

Section: Resultsmentioning

confidence: 65%

“…These results clearly indicate that the genetic coupling of synapsin C domain to LTB constitutes a valid alternative model based probably on bystander suppression of immune response to a structurally unrelated antigen. The protective effect of LTBSC parallels the EAE suppression achieved by administration of a purified synaptosomal fraction (Degano et al, 1998, 2004).…”

Section: Discussionmentioning

confidence: 89%

“…These data prompted us to analyze the effect of intraperitoneal administration of a synaptosomal fraction given prior to the induction of acute EAE. This approach led to the suppression of the clinical signs and also reversed the expected CNS histological and biochemical alterations similarly to the suppression induced by treatment with myelin antigens (Degano et al, 1998, 2004).…”

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confidence: 85%

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Protective effect of a synapsin peptide genetically fused to the B subunit of Escherichia coli heat‐labile enterotoxin in rat autoimmune encephalomyelitis

Scerbo

Rupil

Bibolini

et al. 2009

J of Neuroscience Research

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Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease with similarities to multiple sclerosis that requires the activation of auto reactive T cells that infiltrate the central nervous system. In previous studies we have shown that intraperitoneal administration of synaptosomal antigens could suppress EAE. Herein we examined the effect in this animal model of a fusion protein comprising the C domain of synapsin Ia and the B subunit of Escherichia coli heat-labile enterotoxin (LTBSC). Oral administration to rats of low amounts of LTBSC induced immunological systemic tolerance to the encephalitogenic myelin basic protein. Treatment with LTBSC prior to EAE induction diminished disease incidence, DTH reaction to myelin basic protein, and central nervous system inflammation. LTBSC treatment also reduced the specific T-cell proliferative response to myelin basic protein, decreased nitric oxide production, and augmented arginase activity by peritoneal macrophages. All animals challenged for EAE developed antibody response specific for myelin basic protein, but rats treated with LTBSC showed a lower IgG2b/IgG1 ratio, indicating a shift to a Th2-type milieu. The data presented here suggest that well-conserved synapsin peptides conjugated to the B subunit of enterotoxins from the cholera toxin family have a protective role and provide a potential therapeutic tool for intervention in EAE as well as in multiple sclerosis.

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“…Intravenous, intragastrointestinal and oral administration of MBP are other strategies of suppressing EAE without the use of IFA (see Degano et al . for review) 11 . We have previously shown that i.p.…”

Section: Introductionmentioning

confidence: 99%

Neuronal antigens modulate the immune response associated with experimental autoimmune encephalomyelitis

2004

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Summary Rats primed with bovine myelin (BM) in complete Freunds adjuvant, develop acute experimental autoimmune encephalomyelitis (EAE). We have previously described that intraperitoneal administration prior to the active induction of the disease of a bovine synaptosomal fraction (BSF) and BM were effective ways of suppressing EAE. We found that both treatments diminish the incidence of the disease and reduced biochemical and histological alterations of the central nervous system (CNS). To characterize this suppression process, in this study we examined the antigen-specific immune response in animals protected from EAE. Lymph node mononuclear cells derived from sick EAE rats, as well as from those protected by BM and BSF, showed strong myelin basic protein (MBP) proliferation. Analysis of the humoral response against MBP showed a significant diminution of IgG2b anti-MBP titres in protected BM and BSF rats in contrast to sick EAE rats whose condition could be related to a diminished anti-MBP Th1 response. Finally, cells from rats protected by BSF and BM reduced the incidence of EAE when they were adoptively transferred into animals prior to active induction of the disease. These results suggest that a mechanism based on the generation of regulatory cells and immune deviation could account for the EAE suppression mediated by myelin as well as synaptosomal antigens.

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Suppression of acute experimental allergic encephalomyelitis by intraperitoneal administration of synaptosomal antigens

Cited by 8 publications

References 0 publications

In vivo immunomodulatory effect of the lectin from edible mushroom Agaricus bisporus

In vivo immunomodulatory effect of the lectin from edible mushroom Agaricus bisporus

Protective effect of a synapsin peptide genetically fused to the B subunit of Escherichia coli heat‐labile enterotoxin in rat autoimmune encephalomyelitis

Neuronal antigens modulate the immune response associated with experimental autoimmune encephalomyelitis

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