Suppression of active oxygen-induced cytotoricity by flavonoids

Nakayama, Tsutomu; Yamada, Munetaka; Osawa, Toshihiko; Kawakishi, Shunrô

doi:10.1016/0006-2952(93)90402-i

Cited by 135 publications

(11 citation statements)

References 13 publications

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“…We observed considerable cytotoxicity of quercetin in wild-type cells at relatively high concentrations (0.1 mM) and in a dose-dependent manner. These results are consistent with previous reports of observed flavonoid cytotoxicity in wild-type CHO and non-cancerous human cell lines (27,32). Even better cytotoxicity to BRCA2 mutated cells have been achieved by sufficient active flavonoid concentrations; exposure of high dose of genotoxic flavonoids may have unwanted effects to the healthy tissues.…”

Section: Discussionsupporting

confidence: 94%

Natural and glucosyl flavonoids inhibit poly(ADP-ribose) polymerase activity and induce synthetic lethality in BRCA mutant cells

et al. 2013

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Poly(ADP-ribose) polymerase (PARP) inhibitors have been proven to represent superior clinical agents targeting DNA repair mechanisms in cancer therapy. We investigated PARP inhibitory effects of the natural and synthetic flavonoids (quercetin, rutin, monoglucosyl rutin and maltooligosyl rutin) and tested the synthetic lethality in BRCA2 mutated cells. In vitro ELISA assay suggested that the flavonoids have inhibitory effects on PARP activity, but glucosyl modifications reduced the inhibitory effect. Cytotoxicity tests of Chinese hamster cells defective in BRCA2 gene (V-C8) and its parental V79 cells showed BRCA2-dependent synthetic lethality when treated with the flavonoids. BRCA2 mutated cells were three times more sensitive to the flavonoids than the wild-type and gene complemented cells. Reduced toxicity was observed in a glucosyl modification-dependent manner. The present study provides support for the clinical use of new treatment drugs, and is the beginning of the potential application of flavonoids in cancer prevention and the periodic consumption of appropriate flavonoids to reduce cancer risk in individuals carrying a mutant allele of the BRCA2 gene.

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Section: Discussionsupporting

confidence: 94%

Natural and glucosyl flavonoids inhibit poly(ADP-ribose) polymerase activity and induce synthetic lethality in BRCA mutant cells

et al. 2013

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“…The antioxidant components present in the extract are good electron donors, which may accelerate the reduction of H 2 O 2 to H 2 O. Dietary polyphenols, especially compounds with the orthodihydroxy phenolic structure catechin, quercetin, gallic acid ester, and caffeic acid ester, have been shown to protect mammalian and bacterial cells against hydrogen-peroxide-induced cytotoxicity [33, 34]. Therefore, the phenolic compounds of the hydro-alcoholic extract of Desmostachya bipinnata or other flavonoids present in extract may be responsible for its H 2 O 2 radical scavenging or antioxidant activity.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Antioxidant and DNA Damage Protection Activity of the Hydroalcoholic Extract ofDesmostachya bipinnataL. Stapf

Golla

Bhimathati

2014

The Scientific World Journal

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Desmostachya bipinnata Stapf (Poaceae/Gramineae) is an official drug of ayurvedic pharmacopoeia. Various parts of this plant were used extensively in traditional and folklore medicine to cure various human ailments. The present study was aimed to evaluate the antioxidant and DNA damage protection activity of hydroalcoholic extract of Desmostachya bipinnata both in vitro and in vivo, to provide scientific basis for traditional usage of this plant. The extract showed significant antioxidant activity in a dose-dependent manner with an IC50 value of 264.18 ± 3.47 μg/mL in H2O2 scavenging assay and prevented the oxidative damage to DNA in presence of DNA damaging agent (Fenton's reagent) at a concentration of 50 μg/mL. Also, the presence of extract protected yeast cells in a dose-dependent manner against DNA damaging agent (Hydroxyurea) in spot assay. Moreover, the presence of extract exhibited significant antioxidant activity in vivo by protecting yeast cells against oxidative stressing agent (H2O2). Altogether, the results of current study revealed that Desmostachya bipinnata is a potential source of antioxidants and lends pharmacological credence to the ethnomedical use of this plant in traditional system of medicine, justifying its therapeutic application for free-radical-induced diseases.

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“…Rutin has beneficial effects on hypoxic, glutamate and oxidative stress at concentrations as low as 1 nM on retinal ganglion cell. It appears that the sugar side chain of flavonoids may be important for neuroprotective activities [43]. …”

Section: Discussionmentioning

confidence: 99%

Effects of rutin on acrylamide-induced neurotoxicity

et al. 2014

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BackgroundRutin is an important flavonoid that is consumed in the daily diet. The cytoprotective effects of rutin, including antioxidative, and neuroprotective have been shown in several studies. Neurotoxic effects of acrylamide (ACR) have been established in humans and animals. In this study, the protective effects of rutin in prevention and treatment of neural toxicity of ACR were studied.ResultsRutin significantly reduced cell death induced by ACR (5.46 mM) in time- and dose-dependent manners. Rutin treatment decreased the ACR-induced cytotoxicity significantly in comparison to control (P <0.01, P < 0.001). Rutin (100 and 200 mg/kg) could prevent decrease of body weight in rats. In combination treatments with rutin (50, 100 and 200 mg/kg), vitamin E (200 mg/kg) and ACR, gait abnormalities significantly decreased in a dose-dependent manner (P < 0.01 and P < 0.001). The level of malondialdehyde significantly decreased in the brain tissue of rats in both preventive and therapeutic groups that received rutin (100 and 200 mg/kg).ConclusionIt seems that rutin could be effective in reducing neurotoxicity and the neuroprotective effect of it might be mediated via antioxidant activity.

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Suppression of active oxygen-induced cytotoricity by flavonoids

Cited by 135 publications

References 13 publications

Natural and glucosyl flavonoids inhibit poly(ADP-ribose) polymerase activity and induce synthetic lethality in BRCA mutant cells

Natural and glucosyl flavonoids inhibit poly(ADP-ribose) polymerase activity and induce synthetic lethality in BRCA mutant cells

Evaluation of Antioxidant and DNA Damage Protection Activity of the Hydroalcoholic Extract ofDesmostachya bipinnataL. Stapf

Effects of rutin on acrylamide-induced neurotoxicity

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