Suppression of a thermosensitive dnaA mutation of Escherichia coli by bacteriophage P1 and P7

Chesney, Robert H.; Scott, June R.

doi:10.1016/0147-619x(78)90035-5

Cited by 34 publications

(24 citation statements)

References 48 publications

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“…(188)(189)(190)(191). The integration of certain phages, such as PI (192) and P2sig5 (98), can give a similar result. When the F fa ctor (M. G. Chandler and L. Caro, personal communication), RlOO-l factor (193), or the phage P2sig5 (194,195) is used for this integrative suppression, the origin of chromosome replication in cells growing at high temperature is in the region where the integrated DNA resides.…”

Section: Function Of Required Gene Productsmentioning

confidence: 59%

Initiation of DNA Synthesis in Escherichia Coli

Tomizawa¹,

Selzer²

1979

Annu. Rev. Biochem.

103

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Section: Function Of Required Gene Productsmentioning

confidence: 59%

Initiation of DNA Synthesis in Escherichia Coli

Tomizawa¹,

Selzer²

1979

Annu. Rev. Biochem.

103

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“…Replication of pSC101 is also dependent on the dnaA gene (18,20,31). However, it is not capable of integrative suppression of a temperature-sensitive dnaA allele, in contrast to F (43), R6K (46), and P1 (12,13). These findings suggest that many of the same functions of DnaA protein are required for replication of pSC101 and for the bacterial chromosome.…”

mentioning

confidence: 61%

“…Among the plasmids of E. coli, many require DnaA protein for replication. Of these, F and P1 are capable of integrative suppression of strains containing the dnaA46(Ts) allele (12,13,43) but not a dnaA null allele (28,36). By comparison, the strict dependence of pSC101 on DnaA protein for maintenance (18,20,31) was interpreted to suggest that it requires many of the same functions of dnaA that are required for initiation from oriC.…”

Section: Discussionmentioning

confidence: 99%

Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC

Sutton

Kaguni

1995

J Bacteriol

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Five novel alleles of the Escherichia coli dnaA gene that were temperature sensitive in maintenance of pSC101, a plasmid that is dependent on this gene for replication, were isolated. Nucleotide sequence analysis revealed that four of the five alleles arose from single base substitutions, whereas the fifth contained three base substitutions, two of which were silent. Whereas all five alleles were temperature sensitive in vivo for pSC101 maintenance, genetic and biochemical characterization indicated that only two were defective in replication from the chromosomal origin, oriC. As previously characterized mutations are defective in replication for both pSC101 and oriC, the dnaA mutations specifically defective in pSC101 maintenance represent a novel class. We speculate that one or more of these pSC101-specific mutants are defective in interaction with pSC101 RepA protein, which is also required for initiation of plasmid DNA replication.

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“…Media. Luria broth (LB), tryptone agar, 0 minimal (OM) agar, OM liquid medium, saline diluent and sodium borate buffer have been previously described (Scott, 1974;Chesney & Scott, 1978;Chesney & Adler, 1982;Del Casale et al, 1983). L-Asparagine and sodium aspartate, when used as sole nitrogen sources, were added to a concentration of 5 mM.…”

Section: R H C H E S N E Y P Sollitti a N D D R V I C K Ementioning

confidence: 99%

“…Transductional analysis. Transductions were performed with P 1 cir as previously described (Chesney & Scott, 1978 ; Del Casale et d., 1983). Drug resistance markers were selected on tryptone plates containing the appropriate antibiotic (20 pg ml-I).…”

Section: R H C H E S N E Y P Sollitti a N D D R V I C K Ementioning

confidence: 99%

Identification of a New Locus in the Escherichia coli Cotransduction Gap that Represents a New Genetic Component of the L-Asparagine Utilization System

1985

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A temperature-sensitive Escherichia coli mutant defective for the ability to utilize L-asparagine as a sole nitrogen source was isolated after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis.The mutation (mu) produces two distinct phenotypic effects. (1) Mutant strains grow poorly at high temperature on minimal plates containing asparagine as the sole nitrogen source; this effect is greatly exacerbated by the presence of methionine. (2) Mutant strains utilize Lasparagine as a nitrogen source three to four times more efficiently at permissive temperatures than the wild-type strains. The mutation maps at 32.4 min on the E. coli chromosome, within the E. coli cotransduction gap. Mutant strains produce normal amounts of thermo-stable Lasparaginase I activity. The mutation therefore affects a component of the asparagine utilization system other than the catabolism of asparagine within the cell; it probably affects asparagine uptake.

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Suppression of a thermosensitive dnaA mutation of Escherichia coli by bacteriophage P1 and P7

Cited by 34 publications

References 48 publications

Initiation of DNA Synthesis in Escherichia Coli

Initiation of DNA Synthesis in Escherichia Coli

Novel alleles of the Escherichia coli dnaA gene are defective in replication of pSC101 but not of oriC

Identification of a New Locus in the Escherichia coli Cotransduction Gap that Represents a New Genetic Component of the L-Asparagine Utilization System

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