Suppression of 15-lipoxygenase synthesis by hnRNP E1 is dependent on repetitive nature of LOX mRNA 3′-UTR control element DICE

Reimann, Iris; Huth, Antje; Thiele, Holger; Thiele, B

doi:10.1006/jmbi.2001.5315

Cited by 58 publications

(56 citation statements)

References 34 publications

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“…Only one repeat has been shown to be sufficient for binding of hnRNP E1, which does occur in a stoechiometric manner to the DICE. These findings implicate that at least two hnRNP molecules are required for translational regulation (Reimann et al, 2002).…”

Section: Regulation Of R15-lox Mrna Translation During Erythroid Cellmentioning

confidence: 84%

Control of mRNA translation and stability in haematopoietic cells: The function of hnRNPs K and E1/E2

Ostareck-Lederer

Ostareck

2004

Biology of the Cell

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Studies on the post-transcriptional regulation of gene expression in haematopoietic cells have uncovered that a subfamily of heterogeneous nuclear ribonucleoproteins (hnRNPs) is involved in cytoplasmic gene regulation. HnRNP K and hnRNP E1/E2 share a common structural motif, the hnRNP K homology (KH) domain, which provides a structural basis for mRNA binding. The KH-domains are components of a modular system, which enables these proteins to engage in both, protein/nucleic acid and protein/protein interactions, the latter generating connectivity to cell signalling events. As components of different mRNA-protein complexes, hnRNP K and hnRNP E1/E2 function in the control of mRNA translation and mRNA stability in haematopoietic cell differentiation.

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Section: Regulation Of R15-lox Mrna Translation During Erythroid Cellmentioning

confidence: 84%

Control of mRNA translation and stability in haematopoietic cells: The function of hnRNPs K and E1/E2

Ostareck-Lederer

Ostareck

2004

Biology of the Cell

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“…Precedents for regulation of translation at the step of subunit joining exist in several systems. For example, in early erythroid precursor cells, the mRNA encoding 15-lipoxygenase (r15-LOX) is translationally silenced at this step of translation initiation, dependent on a cytidine-rich 39 UTR element termed DICE (differentiation control element) and on two RNA-binding proteins: hnRNP K and hnRNP E1 (Ostareck et al 2001;Reimann et al 2002;Messias et al 2006). Phosphorylation of a specific tyrosine residue of hnRNP K by c-Src reduces its affinity for DICE, thus activating translation.…”

Section: Discussionmentioning

confidence: 99%

Vasa promotes Drosophila germline stem cell differentiation by activating mei-P26 translation by directly interacting with a (U)-rich motif in its 3′ UTR

Liu

Han²,

Lasko³

2009

Genes Dev.

104

105

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Vasa (Vas) is a DEAD-box RNA-binding protein required in Drosophila at several steps of oogenesis and for primordial germ cell (PGC) specification. Vas associates with eukaryotic initiation factor 5B (eIF5B), and this interaction has been implicated in translational activation of gurken mRNA in the oocyte. Vas is expressed in all ovarian germline cells, and aspects of the vas-null phenotype suggest a function in regulating the balance between germline stem cells (GSCs) and their fate-restricted descendants. We used a biochemical approach to recover Vas-associated mRNAs and obtained mei-P26, whose product represses microRNA activity and promotes GSC differentiation. We found that vas and mei-P26 mutants interact, and that mei-P26 translation is substantially reduced in vas mutant cells. In vitro, Vas protein bound specifically to a (U)-rich motif in the mei-P26 39 untranslated region (UTR), and Vas-dependent regulation of GFP-mei-P26 transgenes in vivo was dependent on the same (U)-rich 39 UTR domain. The ability of Vas to activate mei-P26 expression in vivo was abrogated by a mutation that greatly reduces its interaction with eIF5B. Taken together, our data support the conclusion that Vas promotes germ cell differentiation by directly activating mei-P26 translation in early-stage committed cells.[Keywords: Oogenesis; RNA-binding protein; DEAD-box; patterning; development] Supplemental material is available at http://www.genesdev.org.

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“…26 Indeed, a scan of the D4 sequence for sequences that may modify RNA stability and translational efficiency 27 identified hnRNP K and E binding sites, known as DICE, that are present as a tandem repeated sequence in the 15-lipoxygenase 3 0 untranslated region and suppress translation. 28,29 This identified consensus sequence is present in the region analyzed and includes sequences in the first repeat unit (a sequence). The observation that the single-stranded sense strand of the repeat 72 bp sequence, but not the antisense strand, could block the mobility shift of the nuclear factor (not shown) is consistent with this finding because KH domain proteins, like hnRNP K, can bind to single-stranded DNA with a similar specificity as to their RNA targets.…”

Section: O Schoots and Hhm Van Tolmentioning

confidence: 99%

The human dopamine D4 receptor repeat sequences modulate expression

2003

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Genetic studies have implicated a polymorphic repeat sequence in exon 3 of the human dopamine D4 receptor in various behavioral and psychiatric disorders. Functionally various repeat variants are nearly identical, but whether these have different effects on gene expression has not been studied. To study the role of the repeat sequences on expression independently from its structural and functional effects at the protein level, we introduced these sequences immediately upstream of the promoter and in the 3 0 untranslated region of a luciferase reporter vector. In this report, we demonstrate that the repeat sequence can both modulate promoter activity and alter expression post-transcriptionally. The repeat sequence can serve as a substrate for a nuclear binding factor and all the three repeat variants can suppress promoter activity. Placement of the three repeat variants downstream from the luciferase gene in the expression vector shows, however, that the D4.7 repeat sequence has significantly suppressed expression of the reporter compared to the D4.2 and D4.4 repeats, likely via mechanisms involving RNA stability or translational efficiency. These data indicate that the various D4 repeat sequences have different effects on expression, which may explain its potential role in behavioral disorders.

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Suppression of 15-lipoxygenase synthesis by hnRNP E1 is dependent on repetitive nature of LOX mRNA 3′-UTR control element DICE

Cited by 58 publications

References 34 publications

Control of mRNA translation and stability in haematopoietic cells: The function of hnRNPs K and E1/E2

Control of mRNA translation and stability in haematopoietic cells: The function of hnRNPs K and E1/E2

Vasa promotes Drosophila germline stem cell differentiation by activating mei-P26 translation by directly interacting with a (U)-rich motif in its 3′ UTR

The human dopamine D4 receptor repeat sequences modulate expression

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