Suppression effect on IFN‑γ of adipose tissue‑derived mesenchymal stem cells isolated from β2‑microglobulin‑deficient mice

Abstract

Administration of bone marrow-derived mesenchymal stem cells (MSCs) is a possible treatment for graft-versus-host disease (GVHD) following allogeneic hematopoietic stem cell transplantation and other inflammatory conditions. To address the mechanism of immunosuppression by MSCs, in particular those derived from adipose tissue (AMSCs), AMSCs were isolated from three different mouse strains, and the suppressive capacity of the AMSCs thus obtained to suppress interferon (IFN)-γ generation in mixed lymphocyte reaction cultures serving as an in vitro model of GVHD were assessed. It was revealed that the AMSCs had a potent capacity to suppress IFN-γ production regardless of their strain of origin and that such suppression was not associated with production of interleukin-10. In addition, the results demonstrated that β2-microglobulin (β2m)-deficient AMSCs from β2m-/mice were also potent suppressor cells, verifying the fact that the mechanism underlying the suppression by AMSCs is independent of major histocompatibility complex (MHC) class I expression or MHC compatibility. As AMSCs appear to have immunosuppressive properties, AMSCs may be a useful source of biological suppressor cells for the control of GVHD in humans.

Adipose-Derived Stem Cells Inhibited the Proliferation of Bladder Tumor Cells by S Phase Arrest and Wnt/β-Catenin Pathway

“…Apoptosis and cell cycle arrest are two different mechanisms involved in the induction of cell death (Choi et al, 2018; Jung et al, 2019; Masuda et al, 2018; Salha et al, 2018; Zhang et al, 2010). We found increased apoptosis in T24 cells in the ADSC-CM groups than those in the T24-CM groups and that caspase 3/7 expression was significantly increased.…”

Generation of Mesenchymal Stromal Cells with Low Immunogenicity from Human PBMC-Derived β2 Microglobulin Knockout Induced Pluripotent Stem Cells

“…On the other hand, B2M free, soluble HLA Class I molecules have been shown to inhibit the activity of activated T lymphocytes and even induce apoptosis of T cells 43 , 44 . Along the line, Masuda et al, have reported that adipose tissue-derived MSCs isolated from B2M-deficient mice display a potent capacity to suppress IFN-γ production 45 . It would be worth further investigation in a future study whether the actions of soluble HLA Class I molecules are the underlying mechanism of the enhanced inhibition to T cell proliferation associated with B2MKO iMSCs.…”