Suppression effect of plant-derived berberine on cyprinid herpesvirus 2 proliferation and its pharmacokinetics in Crucian carp (Carassius auratus gibelio)

Su, Meizhen; Tang, Ruizhe; Wang, Hao; Lu, Liqun

doi:10.1016/j.antiviral.2020.105000

Cited by 16 publications

(13 citation statements)

References 37 publications

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Section: Resultsmentioning

confidence: 99%

“…Histological examinations were performed as described previously [ 23 ]; tissues from the fish were dissected, fixed in neutral buffered formalin for 24 h, washed with Dulbecco’s phosphate-buffered saline (DPBS, Sigma, New York, NY, USA), dehydrated with 30% sucrose/DPBS, and embedded in optimum cutting temperature compound (OCT). Eight micrometer sections of all tissue specimens were made using a cryostat (CM1950, Leica, Nussloch, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…The gills of the diseased fish exhibited an extensive fusion of the adjacent lamellae and clear collapse and necrosis (Figure 2D). Thus, the diseased crucian carp exhibited symptoms characteristic of goldfish haematopoietic necrosis virus disease [6,23,27]. Transmission electron microscopy images of kidney cells from naturally diseased crucian carp (Figure 3) showed numerous 150 to 250 nm spherical virions present across the entire cell.…”

Section: Histopathological and Ultrastructural Examination Of Tissues From The Diseased Crucian Carpmentioning

confidence: 99%

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Susceptibility of Goldfish to Cyprinid Herpesvirus 2 (CyHV-2) SH01 Isolated from Cultured Crucian Carp

Wen

et al. 2021

Viruses

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Cyprinid herpesvirus 2 (CyHV-2), a member of the Alloherpesviridae family belonging to the genus Cyprinivirus, is a fatal contagious aquatic pathogen that affects goldfish (Carassius auratus) and crucian carp (Carassius carassius). Although crucian carp and goldfish belong to the genus Carassius, it is unclear whether they are susceptible to the same CyHV-2 isolate. In addition, the origin of the crucian carp-derived CyHV-2 virus isolate remains unclear. CyHV-2 SH01 was isolated during herpesviral hematopoietic necrosis disease (HVHN) outbreaks in crucian carp at a local fish farm near Shanghai. CyHV-2 SH01 was confirmed by PCR and Western blot analysis of kidney, spleen, muscle, and blood tissue from the diseased crucian carp. Moreover, histopathological and ultra-pathological analyses revealed pathological changes characteristic of CyHV-2 SH01 infection in the tissues of the diseased crucian carp. In the present study, goldfish and crucian carp were challenged with CyHV-2 SH01 to elucidate viral virulence. We found that CyHV-2 SH01 could cause rapid and fatal disease progression in goldfish and crucian carp 24 h post-injection at 28 °C. Experimental infection of goldfish by injection indicated that the average virus titer in the kidney of the goldfish was 103.47 to 103.59 copies/mg. In addition, tissues exhibited the most prominent histopathological changes (cellular wrinkling and shrinkage, cytoplasmic vacuolation, fusion of the gill lamellae, and hepatic congestion) in CyHV-2 SH01-infected goldfish and crucian carp. Thus, crucian carp and goldfish showed a high sensitivity, with typical symptoms, to HVHN disease caused by CyHV-2 SH01.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Histopathological and Ultrastructural Examination Of Tissues From The Diseased Crucian Carpmentioning

confidence: 99%

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Susceptibility of Goldfish to Cyprinid Herpesvirus 2 (CyHV-2) SH01 Isolated from Cultured Crucian Carp

Wen

et al. 2021

Viruses

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“…Although infection-initiated oxidative stress plays a key role in the activation of innate immunity to fight off pathogenic microbes, a number of viruses were shown to induce oxidative stress on purpose to facilitate their replication inside the cell, which at least included influenza virus, respiratory syncytial virus (RSV), human metapneumovirus (hMPV), dengue virus (DENV), rotavirus, herpes simplex virus, Zika virus, and HIV (McCord et al, 2020). CyHV-2 infection in crucian carp remains a challenge for the aquaculture section worldwide, and the lack of vaccine or medicine in the field urgently required the development of a novel antiviral strategy (Su et al, 2021). Nrf2 and its principal negative regulator Keap1 play a central role in the maintenance of intracellular redox homeostasis and regulation of inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…For example, the upregulation of intracellular ROS during the early phase of retroviral infection plays an important role in viral establishment in the host cell, and the treatment of apocynin, an inhibitor of NADPH oxidase, could decrease viral titer in the mouse brain and increase the lifespan of infected mice (Kim and Wong, 2013). Recently, we reported that BBR could systematically impede CyHV-2 replication and protect crucian carp from CyHV-2 challenge in a dose-dependent manner (Su et al, 2021). However, it is not clear whether BBR-mediated modulation of ROS signaling involves the anti-CyHV-2 activity of BBR.…”

Section: Introductionmentioning

confidence: 99%

Induction of Reactive Oxygen Species Is Necessary for Efficient Onset of Cyprinid Herpesvirus 2 Replication: Implications for Novel Antiviral Strategy With Antioxidants

Tang

et al. 2022

Front. Microbiol.

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Cyprinid herpesvirus 2 (CyHV-2) has caused great economic loss to the crucian carp breeding industry. Upon viral stimulation, eukaryotic cells generally activate the expression of anti-oxidative genes to maintain the intracellular oxidative balance and resist viral infection. Here, intracellular reactive oxygen species (ROS) levels in CyHV-2-infected cells were monitored to show that CyHV-2 induced the increase of intracellular ROS during early infection, and intracellular excessive accumulation of ROS was ameliorated during late infection, which was accompanied by activated expression of genes related to Nrf2 signaling pathway. In order to explore the interaction between CyHV-2 infection and ROS production, RyuF-2 cells were treated with either antioxidant epigallocatechin-3-gallate (EGCG) or berberine hydrochloride (BBH) and then infected with CyHV-2. Both BBH and EGCG could effectively inhibit the amplification of CyHV-2 while inhibiting the accumulation of intracellular ROS. Consistent with this, the oxidant stress-related genes were up-regulated by CyHV-2 infection and down-regulated in cells treated with either BBH or EGCG, through which the production of intracellular ROS was modulated. These results collectively demonstrated that early ROS accumulation favored the replication of CyHV-2, while antioxidants (BBH and EGCG) could inhibit the amplification of CyHV-2 by inhibiting ROS induction.

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Intrinsic D614G and P681R/H mutations in SARS-CoV-2 VoCs Alpha, Delta, Omicron and viruses with D614G plus key signature mutations in spike protein alters fusogenicity and infectivity

Khatri

Siddqui

Sadhu

et al. 2022

Med Microbiol Immunol

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The SARS-CoV-2 virus has been rapidly evolving over the time and the genetic variation has led to the generation of Variants of Concerns (VoC), which have shown increased fitness. These VoC viruses contain the key mutations in the spike protein which have allowed better survival and evasion of host defense mechanisms. The D614G mutation in the spike domain is found in the majority of VoC; additionally, the P681R/H mutation at the S1/S2 furin cleavage site junction is also found to be highly conserved in major VoCs; Alpha, Delta, Omicron, and its’ current variants. The impact of these genetic alterations of the SARS-CoV-2 VoCs on the host cell entry, transmissibility, and infectivity has not been clearly identified. In our study, Delta and D614G + P681R synthetic double mutant pseudoviruses showed a significant increase in the cell entry, cell-to-cell fusion and infectivity. In contrast, the Omicron and P681H synthetic single mutant pseudoviruses showed TMPRSS2 independent cell entry, less fusion and infectivity as compared to Delta and D614G + P681R double mutants. Addition of exogenous trypsin further enhanced fusion in Delta viruses as compared to Omicron. Furthermore, Delta viruses showed susceptibility to both E64d and Camostat mesylate inhibitors suggesting, that the Delta virus could exploit both endosomal and TMPRSS2 dependent entry pathways as compared to the Omicron virus. Taken together, these results indicate that the D614G and P681R/H mutations in the spike protein are pivotal which might be favoring the VoC replication in different host compartments, and thus allowing a balance of mutation vs selection for better long-term adaptation.

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Suppression effect of plant-derived berberine on cyprinid herpesvirus 2 proliferation and its pharmacokinetics in Crucian carp (Carassius auratus gibelio)

Cited by 16 publications

References 37 publications

Susceptibility of Goldfish to Cyprinid Herpesvirus 2 (CyHV-2) SH01 Isolated from Cultured Crucian Carp

Susceptibility of Goldfish to Cyprinid Herpesvirus 2 (CyHV-2) SH01 Isolated from Cultured Crucian Carp

Induction of Reactive Oxygen Species Is Necessary for Efficient Onset of Cyprinid Herpesvirus 2 Replication: Implications for Novel Antiviral Strategy With Antioxidants

Intrinsic D614G and P681R/H mutations in SARS-CoV-2 VoCs Alpha, Delta, Omicron and viruses with D614G plus key signature mutations in spike protein alters fusogenicity and infectivity

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