Suppression by the ColV, I-K94 Plasmid of a Growth Lesion in ompA Mutants of Escherichia coli

Deeney, Caroline M. M.; Goodson, Margaret; Rossouw, F.T.; Rowbury, R. J.

doi:10.1099/00221287-132-8-2287

Cited by 3 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3). The results are surface (Deeney et al 1986) and at the periplasmic face of the shown here for ED1829 and its ColV,I-K94+ derivative, but outer membrane (Moores and Rowbury 1982). P678-54 ColV,I-K94 and RJR203 showed similar effects.…”

Section: Levels O F the Majorproteins In The Envelopes Of Mutants Andsupporting

confidence: 76%

“…A major fragment might have been masked by another envelope component. Alternatively, if 3 4 protease X had cleaved several exposed loops, small fragments only, which would not have been detected on our gels, ColV,I-K94+ strain from RJR200 contained no traces of OmpA protein nor was it sensitive to phage K3, and K3hl or to colicin L (Deeney et al 1986). Additionally, the cured strain (RJR204) behaved like the ompA parent in sensitivity to antibacterials ( Table 2).…”

Section: ' -!mentioning

confidence: 57%

“…The suppressing effect of ColV,I-K94 may be due to the presence of the VmpA protein because ColV-8 and ColV-41, which do not encode this protein, failed to suppress the EDTA or antibiotic sensitivities, while ColV-M40(5) which confers a greatly reduced level of VmpA protein (Deeney et al 1986) allowed growth in the presence of EDTA but not in the presence of the other two agents. One ColV, from a clinical isolate, (ColV-2/5656) was transferred to an ompA mutant (HB101 ompA) and its suppression of the inhibitor sensitivities of this mutant (Table 6) is associated with its encoding of the VmpA protein .…”

Section: ' -!mentioning

confidence: 94%

“…Further comparison of the ompA and vmpA genes and their products Previous studies have indicated some similarities between the OmpA and VmpA gene products and many differences (Moores and Rowbury 1982;Rowbury et al 1985;Deeney et al 1986). Several other differences have now been established.…”

Section: Levels O F the Majorproteins In The Envelopes Of Mutants Andmentioning

confidence: 97%

“…Because the ColV,I-K94 plasmid will suppress a growth lesion in ompA mutants (Deeney et al 1986), its effect on antibiotic sensitivity was tested. All three ompA mutants tested showed increased resistance to EDTA, polymyxin B, and gentamicin on introduction of ColV,I-K94 (Table 2), and for RJR200, the plasmid conferred increased resistance to all the other tested inhibitory agents except ampicillin (Tables 3 Sensitivity to hydrophobic agents and 4).…”

Section: Suppression Of Antibiotic Sensitivity By Colvi-k94mentioning

confidence: 99%

See 4 more Smart Citations

Suppression by the ColV,I-K94 plasmid of inhibitor sensitivities in ompA mutants of Escherichia coli

Reakes¹,

Deeney²,

Goodson³

et al. 1988

Can. J. Microbiol.

View full text Add to dashboard Cite

A series of ompA mutants derived from Escherichia coli K12 strains showed increased sensitivity (compared with the ompA+ parents) to aminoglycoside antibiotics and to other cationic agents including polymyxin B. One tested mutant also showed increased sensitivity to nafcillin and fusidic acid, but not to the hydrophilic ampicillin. All these inhibitor sensitivities in the ompA mutants were suppressed by ColV, I-K94 and by certain other ColV plasmids, but not by any of the other tested large plasmids. Suppression correlated with the production of the VmpA protein, but transfer and colicin components were not needed for suppression. Further comparison of the ompA and vmpA genes and their products was made and it indicated that there is little if any homology between the genes, that the synthesis of their products is regulated by quite different mechanisms, and that regions of these gene products exposed at the cell surface show different susceptibility to protease attack after denaturation.

show abstract

Section: Levels O F the Majorproteins In The Envelopes Of Mutants Andsupporting

confidence: 76%

Section: ' -!mentioning

confidence: 57%

Section: ' -!mentioning

confidence: 94%

Section: Levels O F the Majorproteins In The Envelopes Of Mutants Andmentioning

confidence: 97%

Section: Suppression Of Antibiotic Sensitivity By Colvi-k94mentioning

confidence: 99%

See 3 more Smart Citations

Suppression by the ColV,I-K94 plasmid of inhibitor sensitivities in ompA mutants of Escherichia coli

Reakes¹,

Deeney²,

Goodson³

et al. 1988

Can. J. Microbiol.

View full text Add to dashboard Cite

show abstract

Acid resistance of attached organisms and its implications for the pathogenicity of plasmid-bearing Escherichia coli

Poynter

Hicks

Rowbury

1986

Lett Appl Microbiol

Self Cite

View full text Add to dashboard Cite

1986. Acid resistance of attached organisms and its implications for the pathogenicity of plasmid-bearing Escherichia coli. Letters in Applied Microbiology 3, 117-121.Organisms of Escherichia coli attached to glass beads in a model attachment system were more resistant to acid than were unattached organisms and this applied to cultures exposed to either pH 2.5 or 3.5. Attached organisms of both Col-and COW, I-K94' strains showed the effect but with prolonged exposure to pH 2.5, the attached COW' organisms appeared more resistant than the attached Colones, possibly because they formed a thicker surface layer. It is proposed that the increased resistance of attached organisms to pH 2.5 might allow the survival, in gastric acid, of organisms attached to food particles. This would be more significant for the COW' strains because the plasmid enhances enterobacterial attachment properties. The increased resistance of attached organisms to pH 3.5 might be significant for survival of particle-or surface-bound organisms in the acidic environment of the phagocyte especially since lactate (which occurs in phagocytes) enhanced the effect of exposure to pH 3.5.

show abstract

The ColV, I-K94 plasmid and heat sensitivity of Escherichia coli

Ghazaleh

Humphrey

Smith

et al. 1989

Lett Appl Microbiol

View full text Add to dashboard Cite

The ColV,I-K94 plasmid and heat sensitivity of Escherchia coli. Letters in Applied Microbiology 9 , 7 5 7 7 .The ColV,I-K94 plasmid markedly increased the heat sensitivity of Escherichia coli K12 but had no sensitizing effect on a wild E. coli isolate. The plasmid effect on strain K12 appeared to result from ColV-encoded transfer and colicin components possibly due to their effects on membrane properties.

show abstract

Suppression by the ColV, I-K94 Plasmid of a Growth Lesion in ompA Mutants of Escherichia coli

Cited by 3 publications

References 17 publications

Suppression by the ColV,I-K94 plasmid of inhibitor sensitivities in ompA mutants of Escherichia coli

Suppression by the ColV,I-K94 plasmid of inhibitor sensitivities in ompA mutants of Escherichia coli

Acid resistance of attached organisms and its implications for the pathogenicity of plasmid-bearing Escherichia coli

The ColV, I-K94 plasmid and heat sensitivity of Escherichia coli

Contact Info

Product

Resources

About