Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells

Liu, Guanqi; Lu, Yeming; Mai, Zhihui; Liu, Runheng; Peng, Zhuli; Chen, Lin; Chen, Zheng; Wang, Ruizhi; Hong, Ai

doi:10.1155/2019/7547506

Cited by 14 publications

(13 citation statements)

References 51 publications

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“…Through bioinformatics analysis, we found a regulatory relationship between the differentially expressed genes and microRNAs after E2 treatment. miRNA has been widely reported to be involved in E2 regulation (20)(21)(22). Through miRNA-target gene prediction, we found miR-148a and miR-152 have binding sequences on the 3'UTR of ADCY2 (Fig.…”

Section: E2 Promotes Adcy2 Expression Through Inhibiting Mir-148a/mirmentioning

confidence: 90%

Estrogen Promotes cAMP Production in Mesenchymal Stem Cells by Regulating ADCY2

Zhao

Miao

et al. 2020

IJSC

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Background and Objectives: The maternal-fetal interface is an important source of mesenchymal stem cells (MSCs), and it is influenced by high levels of estradiol (E2) during pregnancy. It is highly important to study the role of E2 in MSCs for both clinical application and understanding of the mechanisms underlying pregnancy related diseases. Methods and Results: In this study, differently expressed genes (DEGs) were found in the MSCs after exposure to E2. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of DEGs was performed and the integrated regulatory network of DEGs-miRNA was constructed. A total of 390 DEGs were found in the MSCs exposed to E2, including 164 upregulated DEGs (e.g. ADCY2, VEGFA and PPY) and 226 downregulated DEGs (e.g. KNG1, AGT and NPY). Additionally, 10 miRNAs (such as miR-148A/B, miR-152, miR-182) identified the integrated regulatory network of DEGs-miRNAs. Among them, the expression of ADCY2 was significantly upregulated, and this was associated with multiple changed genes. We confirmed that the expression of ADCY2 is significantly promoted by E2 and subsequently promoted the production of cAMP in MSCs. We also found that E2 promoted ADCY2 expression by inhibiting miR-152 and miR-148a. Conclusions: E2 promotes the expression of cAMP through miR-148a/152-ADCY2 in MSCs. It is suggested that E2 plays a key role in the growth and function of MSCs.

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Section: E2 Promotes Adcy2 Expression Through Inhibiting Mir-148a/mirmentioning

confidence: 90%

Estrogen Promotes cAMP Production in Mesenchymal Stem Cells by Regulating ADCY2

Zhao

Miao

et al. 2020

IJSC

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“…The isolation and culture of rBMSCs from the tibias and femurs of 3-week-old male Sprague-Dawley rats were performed as described previously [ 22 , 23 ]. Briefly, rats were sacrificed by cervical dislocation and sterilized with 75% ethanol.…”

Section: Methodsmentioning

confidence: 99%

Dopamine Suppresses Osteogenic Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells via AKT/GSK-3β/β-Catenin Signaling Pathway

Kuang

Chen

et al. 2022

Stem Cells International

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Nervous system is critically involved in bone homeostasis and osteogenesis. Dopamine, a pivotal neurotransmitter, plays a crucial role in sympathetic regulation, hormone secretion, immune activation, and blood pressure regulation. However, the role of dopamine on osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) remains poorly understood. In this study, we firstly investigated the effect of dopamine on the apoptosis, proliferation, and osteogenic differentiation of rBMSCs. Dopamine did not, however, interfere with the apoptosis and proliferation of rBMSCs. Interestingly, dopamine suppressed the osteogenic differentiation of rBMSCs, as characterized by reduced ALP staining, ALP activity, mineralized nodule formation, and the mRNA and protein levels of osteogenesis-related genes (Col1a1, Alp, Runx2, Opn, and Ocn). Furthermore, dopamine inactivated AKT/GSK-3β/β-catenin signaling pathway. Treatment of LiCl (GSK-3β inhibitor) rescued the inhibitory effects of dopamine on osteogenic differentiation of rBMSCs. LY294002 (AKT inhibitor) administration exacerbated the inhibitory effects of dopamine on osteogenic differentiation of rBMSCs. Taken together, these findings indicate that dopamine suppresses osteogenic differentiation of rBMSCs via AKT/GSK-3β/β-catenin signaling pathway. Our study provides new insights into the role of neurotransmitters in bone homeostasis.

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“…Most of the miRNAs reported above have been investigated in different studies, confirming or not their role in osteogenic differentiation [135,151,[165][166][167][168]. Great interest was shown for miR-23a, which was found to be overexpressed in mouse non-transformed osteoblastic cell line MC3T3-E1, inhibiting osteogenic differentiation [162,169].…”

Section: Transcription Factor Runx2mentioning

confidence: 98%

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

Mazziotta

Lanzillotti

Iaquinta

et al. 2021

IJMS

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Mesenchymal stem cells (MSCs) have been identified in many adult tissues and they have been closely studied in recent years, especially in view of their potential use for treating diseases and damaged tissues and organs. MSCs are capable of self-replication and differentiation into osteoblasts and are considered an important source of cells in tissue engineering for bone regeneration. Several epigenetic factors are believed to play a role in the osteogenic differentiation of MSCs, including microRNAs (miRNAs). MiRNAs are small, single-stranded, non-coding RNAs of approximately 22 nucleotides that are able to regulate cell proliferation, differentiation and apoptosis by binding the 3′ untranslated region (3′-UTR) of target mRNAs, which can be subsequently degraded or translationally silenced. MiRNAs control gene expression in osteogenic differentiation by regulating two crucial signaling cascades in osteogenesis: the transforming growth factor-beta (TGF-β)/bone morphogenic protein (BMP) and the Wingless/Int-1(Wnt)/β-catenin signaling pathways. This review provides an overview of the miRNAs involved in osteogenic differentiation and how these miRNAs could regulate the expression of target genes.

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Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow Mesenchymal Stem Cells

Cited by 14 publications

References 51 publications

Estrogen Promotes cAMP Production in Mesenchymal Stem Cells by Regulating ADCY2

Estrogen Promotes cAMP Production in Mesenchymal Stem Cells by Regulating ADCY2

Dopamine Suppresses Osteogenic Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells via AKT/GSK-3β/β-Catenin Signaling Pathway

MicroRNAs Modulate Signaling Pathways in Osteogenic Differentiation of Mesenchymal Stem Cells

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