Supportive Role of Cellular Bridge of Neurons Expressing a Highly Polysialylated Form of NCAM (NCAM-H) at the Initial Stage of Migration of LHRH Neurons

Miyakawa, Momoko; Seki, Tatsunori; Arai, Yasumasa

doi:10.2108/zsj.14.489

Cited by 5 publications

(12 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was observed at E3 in chicks (Murakami et al. , 1991; Norgren & Brackenbury, 1993 and Miyakawa et al. , 1997), E10 in mice (Schwanzel‐Fukuda et al.…”

Section: Psa‐ncam and The Developing Gnrh Systemmentioning

confidence: 84%

“…The spatiotemporal relationship of GnRH and PSA‐NCAM has been examined by immunohistochemical procedures across normal embryonic development in chicks (Murakami et al. , 1991, 2000; Norgren & Brackenbury, 1993; Miyakawa et al. , 1997), mice (Schwanzel‐Fukuda et al.…”

Section: Psa‐ncam and The Developing Gnrh Systemmentioning

confidence: 99%

“…Much more debated is the possibility that migrating GnRH neurons themselves express PSA‐NCAM. On the basis of double immunofluorescent labeling of 10‐μm‐thick cryosections of chick embryos, Miyakawa et al. (1997) considered that GnRH neurons did express PSA‐NCAM from the time that they were first detected in the olfactory pit at E3.5.…”

Section: Identity Of the Neural Elements Expressing Psa‐ncam Along Thmentioning

confidence: 99%

See 2 more Smart Citations

The intimate relationship of gonadotropin‐releasing hormone neurons with the polysialylated neural cell adhesion molecule revisited across development and adult plasticity

Franceschini

Desroziers

Caraty

et al. 2010

Eur J of Neuroscience

View full text Add to dashboard Cite

The neurohormone gonadotropin-releasing hormone (GnRH) is critical for all the aspects of reproductive life in vertebrates. GnRH is secreted by a small number of neurons dispersed within the preoptic-hypothalamic region. These neurons are derived from the embryonic olfactory pit. They then migrate along olfactory, vomeronasal and terminal nerves to their final destination. Classical approaches to study the regulation of GnRH secretion during the reproductive cycle have focused on the various neuronal inputs on GnRH neurons and their regulation by ovarian steroids. However, it is well known that steroids will change the microenvironment of neuronal networks and can induce plasticity and functional changes. In this review, we will focus on the intimate relationship of developing and adult GnRH neurons with the polysialylated form of neural cell adhesion molecule (PSA-NCAM), a major molecular actor in the morphogenesis and adult plasticity of the nervous system. We will first recapitulate the spatiotemporal relationship between PSA-NCAM and migrating GnRH neurons during embryogenesis of various vertebrate species and discuss its importance for GnRH neuron development as shown by various loss of function studies. In the adult, we will review the relationships between PSA-NCAM and GnRH neurons across various physiological states, and open the discussion to the use of new model systems that can help to unravel the function and mechanism of action of PSA-NCAM on GnRH neuronal network activity and GnRH release.

show abstract

“…This was observed at E3 in chicks (Murakami et al. , 1991; Norgren & Brackenbury, 1993 and Miyakawa et al. , 1997), E10 in mice (Schwanzel‐Fukuda et al.…”

Section: Psa‐ncam and The Developing Gnrh Systemmentioning

confidence: 84%

Section: Psa‐ncam and The Developing Gnrh Systemmentioning

confidence: 99%

Section: Identity Of the Neural Elements Expressing Psa‐ncam Along Thmentioning

confidence: 99%

See 1 more Smart Citation

The intimate relationship of gonadotropin‐releasing hormone neurons with the polysialylated neural cell adhesion molecule revisited across development and adult plasticity

Franceschini

Desroziers

Caraty

et al. 2010

Eur J of Neuroscience

View full text Add to dashboard Cite

show abstract

“…SS mRNA‐expressing cells coexpressed HuC/D (Figure 1, c 1–3), and the proportion of SS mRNA‐expressing cells to the total number of HuC/D‐positive cells in the mesenchymal tissue was 38.7% ( n = 1 embryo). At E3.5 (HH18−20), it has been demonstrated that early migratory cells from the OP form a cellular cord between the epithelium of the olfactory pit and the rostral telencephalon (Drapkin & Silverman, 1999; Miyakawa et al., 1997). The number of SS mRNA‐expressing cells increased not only in the epithelium of the olfactory pit but also in the HuC/D‐positive cellular cord (Figure 1d, e1–3).…”

Section: Resultsmentioning

confidence: 99%

“…Although early migratory cells from the OP at E3 (HH17−18) express immature and mature neuronal markers (Drapkin & Silverman, 1999;Miyakawa et al, 1997), we examined whether OP-derived cells that were labeled at E3 were part of the neuronal population. We analyzed an E17 (HH43) embryo with a relatively large number of GFP-labeled cells in the brain and found that a large proportion of GFP-labeled cells expressed the mature neuronal marker NeuN (73.3%, n = 1 embryo, Figure 6a We further examined the molecular properties of GFP-labeled GnRH-negative cells in the forebrain using triple immunofluorescence staining with GFP, GnRH, and NPY or with CB antibodies.…”

Section: Phenotypes Of Long-lasting Gfp-labeled Op-derived Cells In T...mentioning

confidence: 99%

Olfactory placode generates a diverse population of neurons expressing GnRH, somatostatin mRNA, neuropeptide Y, or calbindin in the chick forebrain

Murakami

Ohki‐Hamazaki

Uchiyama

2022

J of Comparative Neurology

View full text Add to dashboard Cite

The olfactory placode (OP) of vertebrates generates several classes of migrating cells, including hypothalamic gonadotropin-releasing hormone (GnRH)-producing neurons, which play essential roles in the reproduction system. Previous studies using OP cell labeling have demonstrated that OP-derived non-GnRH cells enter the developing forebrain; however, their final fates and phenotypes are less well understood. In chick embryos, a subpopulation of migratory cells from the OP that is distinct from GnRH neurons transiently expresses somatostatin (SS). We postulated that these cells are destined to develop into brain neurons. In this study, we examined the expression pattern of SS mRNA in the olfactory-forebrain region during development, as well as the destination of OP-derived migratory cells, including SS mRNA-expressing cells. Utilizing the Tol2 genomic integration system to induce long-term fluorescent protein expression in OP cells, we found that OP-derived migratory cells labeled at embryonic day (E) 3 resided in the olfactory nerve and medial forebrain at E17-19. A subpopulation of green fluorescent protein (GFP)-labeled GnRH neurons that remained in the olfactory nerve was considered to comprise terminal nerve neurons. In the forebrain, GFP-labeled cells showed a distribution pattern similar to that of GnRH neurons. A large proportion of GFP-labeled cells expressed the mature neuronal marker NeuN.Among the GFP-labeled cells, the percentage of GnRH neurons was low, while the remaining GnRH-negative neurons either expressed SS mRNA, neuropeptide Y, or calbindin D-28k or did not express any of them. These results indicate that a diverse population of OP-derived neuronal cells, other than GnRH neurons, integrates into the chick medial forebrain.

show abstract

Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos

Miyakawa

Murakmai

Uchiyama

2022

The Anatomical Record

Self Cite

View full text Add to dashboard Cite

Cell migration from the olfactory placode (OP) is a well-known phenomenon wherein various cell types, such as gonadotropin-releasing hormone (GnRH)producing neurons, migrate toward the telencephalon (TEL) during early embryonic development. However, the spatial relationship between early migratory cells and the forebrain is unclear. We examined the early development of whole-mount chick embryos to observe the three-dimensional spatial relationship among OP-derived migratory neurons, olfactory nerve (ON), and TEL. Migratory neurons that express highly polysialylated neural cell adhesion molecule (PSA-NCAM) emerge from the OP and spread over a relatively wide TEL area at the Hamburger and Hamilton (HH) stage 17. Most migratory neurons form a cellular cord between the olfactory pit and rostral TEL within HH18-20. The earliest axons from the olfactory epithelium (OE) were detected along this neuronal cord using DiI-labeling at HH21. Furthermore, a few PSA-NCAM-positive neurons were dispersed around the cellular cord and over the lateral TEL at HH18. A long cellular cord branch extending to the lateral TEL was transiently observed within HH18-24. These results suggest a novel migratory route of OP-derived neurons during the early developmental stages. Following GFP vector introduction into the OP of HH13-15 embryos, labeled neurons were detected around and within the lateral TEL at HH23 and HH27. At HH36, labeled cells were observed in the rostral-lateral TEL, including the olfactory bulb (OB) region. GFP-labeled and calretinin-positive neurons were detected in the OB, suggesting that early OP-derived neurons enter the forebrain and function as interneurons in the OB.

show abstract

Supportive Role of Cellular Bridge of Neurons Expressing a Highly Polysialylated Form of NCAM (NCAM-H) at the Initial Stage of Migration of LHRH Neurons

Cited by 5 publications

References 19 publications

The intimate relationship of gonadotropin‐releasing hormone neurons with the polysialylated neural cell adhesion molecule revisited across development and adult plasticity

The intimate relationship of gonadotropin‐releasing hormone neurons with the polysialylated neural cell adhesion molecule revisited across development and adult plasticity

Olfactory placode generates a diverse population of neurons expressing GnRH, somatostatin mRNA, neuropeptide Y, or calbindin in the chick forebrain

Wide‐ranging migration and destination of early olfactory placode‐derived neurons in chick embryos

Contact Info

Product

Resources

About