Supplemented phase diagrams for vitrification CPA cocktails: DP6, VS55 and M22

Han, Zonghu; Gangwar, Lakshya; Magnuson, Elliott C.; Etheridge, Michael L.; Pringle, C O; Bischof, John C.; Choi, Jeung Hwan

doi:10.1016/j.cryobiol.2022.02.005

Cited by 6 publications

(3 citation statements)

References 75 publications

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“…CCR and CWR were calculated from the measured heat flow using ice fractions following previously published methods 48 , 49 . T m (peak) and T g (inflection point) of heating were calculated using the heating cycle of DSC heat flow curves thermogram 50 .…”

Section: Methodsmentioning

confidence: 99%

Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model

et al. 2023

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Banking cryopreserved organs could transform transplantation into a planned procedure that more equitably reaches patients regardless of geographical and time constraints. Previous organ cryopreservation attempts have failed primarily due to ice formation, but a promising alternative is vitrification, or the rapid cooling of organs to a stable, ice-free, glass-like state. However, rewarming of vitrified organs can similarly fail due to ice crystallization if rewarming is too slow or cracking from thermal stress if rewarming is not uniform. Here we use “nanowarming,” which employs alternating magnetic fields to heat nanoparticles within the organ vasculature, to achieve both rapid and uniform warming, after which the nanoparticles are removed by perfusion. We show that vitrified kidneys can be cryogenically stored (up to 100 days) and successfully recovered by nanowarming to allow transplantation and restore life-sustaining full renal function in nephrectomized recipients in a male rat model. Scaling this technology may one day enable organ banking for improved transplantation.

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Section: Methodsmentioning

confidence: 99%

Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model

et al. 2023

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“…The critical cooling rate (CCR) and the critical warming rate (CWR) are the minimum rates required to suppress devitrification and ice formation, which depend on the composition and concentration of the CPA used. 32 VS55 is a commonly used CPA, consisting of 3.1 M dimethyl sulfoxide (DMSO), 2.2 M propylene glycol, and 3.1 M formamide (8.4 M in total), as well as salts and sugars as osmotic buffers. The CCR and CWR for VS55 are À2.5 and 50 1C min À1 , respectively.…”

Section: Balancing Heating Rate and Acute Toxicitymentioning

confidence: 99%

Magnetic nanoparticles for nanowarming: seeking a fine balance between heating performance and biocompatibility

Liu

Yin

2023

Mater. Chem. Front.

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“…Successful vitrification requires a cooling rate higher than the critical cooling rate (CCR), which is dictated by the choice of CPA and its concentration. [19][20][21] Cooling at a rate slower than the CCR results in destructive ice formation. Low CPA concentrations require a higher CCR to achieve vitrification.…”

Section: Introductionmentioning

confidence: 99%

Conduction‐Dominated Cryomesh for Organism Vitrification

Guo,

Zuchowicz,

Bouwmeester

et al. 2023

Advanced Science

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Vitrification‐based cryopreservation is a promising approach to achieving long‐term storage of biological systems for maintaining biodiversity, healthcare, and sustainable food production. Using the “cryomesh” system achieves rapid cooling and rewarming of biomaterials, but further improvement in cooling rates is needed to increase biosystem viability and the ability to cryopreserve new biosystems. Improved cooling rates and viability are possible by enabling conductive cooling through cryomesh. Conduction‐dominated cryomesh improves cooling rates from twofold to tenfold (i.e., 0.24 to 1.2 × 105 °C min−1) in a variety of biosystems. Higher thermal conductivity, smaller mesh wire diameter and pore size, and minimizing the nitrogen vapor barrier (e.g., vertical plunging in liquid nitrogen) are key parameters to achieving improved vitrification. Conduction‐dominated cryomesh successfully vitrifies coral larvae, Drosophila embryos, and zebrafish embryos with improved outcomes. Not only a theoretical foundation for improved vitrification in µm to mm biosystems but also the capability to scale up for biorepositories and/or agricultural, aquaculture, or scientific use are demonstrated.

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Supplemented phase diagrams for vitrification CPA cocktails: DP6, VS55 and M22

Cited by 6 publications

References 75 publications

Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model

Vitrification and nanowarming enable long-term organ cryopreservation and life-sustaining kidney transplantation in a rat model

Magnetic nanoparticles for nanowarming: seeking a fine balance between heating performance and biocompatibility

Conduction‐Dominated Cryomesh for Organism Vitrification

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