Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover

Qing, Qing; Wyman, Charles E.

doi:10.1186/1754-6834-4-18

Cited by 205 publications

(107 citation statements)

References 38 publications

(47 reference statements)

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“…Interestingly, coculturing revealed clear synergistic effects, with the synergy factor reaching 1.8 for combinations with a large excess of the xylanase. These results suggest that the action of the xylanase in deconstructing the substrate renders the cellulose accessible to the cellulase, as described in previous studies (44)(45)(46).…”

Section: Discussionsupporting

confidence: 56%

Establishment of a Simple Lactobacillus plantarum Cell Consortium for Cellulase-Xylanase Synergistic Interactions

Moraïs

Shterzer

Grinberg

et al. 2013

Appl Environ Microbiol

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Lactobacillus plantarum is an attractive candidate for bioprocessing of lignocellulosic biomass due to its high metabolic variability, including its ability to ferment both pentoses and hexoses, as well as its high acid tolerance, a quality often utilized in industrial processes. This bacterium grows naturally on biomass; however, it lacks the inherent ability to deconstruct lignocellulosic substrates. As a first step toward engineering lignocellulose-converting lactobacilli, we have introduced genes coding for a GH6 cellulase and a GH11 xylanase from a highly active cellulolytic bacterium into L. plantarum. For this purpose, we employed the recently developed pSIP vectors for efficient secretion of heterologous proteins. Both enzymes were secreted by L. plantarum at levels estimated at 0.33 nM and 3.3 nM, for the cellulase and xylanase, respectively, in culture at an optical density at 600 nm (OD 600 ) of 1. Transformed cells demonstrated the ability to degrade individually either cellulose or xylan and wheat straw. When mixed together to form a two-strain cell-based consortium secreting both cellulase and xylanase, they exhibited synergistic activity in the overall release of soluble sugar from wheat straw. This result paves the way toward metabolic harnessing of L. plantarum for novel biorefining applications, such as production of ethanol and polylactic acid directly from plant biomass.

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Section: Discussionsupporting

confidence: 56%

Establishment of a Simple Lactobacillus plantarum Cell Consortium for Cellulase-Xylanase Synergistic Interactions

Moraïs

Shterzer

Grinberg

et al. 2013

Appl Environ Microbiol

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“…The synergistic action of xylanase with cellulases has already been demonstrated in earlier studies using corn stover [15,27,30-32]. Effects were more important on AFEX-treated corn stover as this substrate has a higher xylan content, but were also clear on the dilute acid pretreated substrates having less than 6% xylan [27,31]. Mixture optimization on AFEX-treated corn stover resulted in optimal xylanase contents of 13% to 22% [20,33].…”

Section: Resultsmentioning

confidence: 81%

Optimization of a synthetic mixture composed of major Trichoderma reesei enzymes for the hydrolysis of steam-exploded wheat straw

Billard

Faraj

Ferreira

et al. 2012

Biotechnol Biofuels

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BackgroundAn efficient hydrolysis of lignocellulosic substrates to soluble sugars for biofuel production necessitates the interplay and synergistic interaction of multiple enzymes. An optimized enzyme mixture is crucial for reduced cost of the enzymatic hydrolysis step in a bioethanol production process and its composition will depend on the substrate and type of pretreatment used. In the present study, an experimental design was used to determine the optimal composition of a Trichoderma reesei enzyme mixture, comprising the main cellulase and hemicellulase activities, for the hydrolysis of steam-exploded wheat straw.MethodsSix enzymes, CBH1 (Cel7a), CBH2 (Cel6a), EG1 (Cel7b), EG2 (Cel5a), as well as the xyloglucanase Cel74a and the xylanase XYN1 (Xyl11a) were purified from a T. reesei culture under lactose/xylose-induced conditions. Sugar release was followed in milliliter-scale hydrolysis assays for 48 hours and the influence of the mixture on initial conversion rates and final yields is assessed.ResultsThe developed model could show that both responses were strongly correlated. Model predictions suggest that optimal hydrolysis yields can be obtained over a wide range of CBH1 to CBH2 ratios, but necessitates a high proportion of EG1 (13% to 25%) which cannot be replaced by EG2. Whereas 5% to 10% of the latter enzyme and a xylanase content above 6% are required for highest yields, these enzymes are predicted to be less important in the initial stage of hydrolysis.ConclusionsThe developed model could reliably predict hydrolysis yields of enzyme mixtures in the studied domain and highlighted the importance of the respective enzyme components in both the initial and the final hydrolysis phase of steam-exploded wheat straw.

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“…Thus, xylanases were needed for effective hydrolysis. By adding xylanases, the hemicellulose barrier around cellulose was removed, which increased the accessibility of cellulose to cellulases (Hu et al 2011;Qing and Wyman 2011). To investigate whether the enzyme dosage ratio of CMax and AF100L had an effect on the hydrolysis of wood pulp, sugar production from the reduced 10 mg/g dry mass of CMax was then investigated with different amounts of AF100L.…”

Section: Effects Of Enzyme Dosage On the Enzymatic Hydrolysismentioning

confidence: 99%

Optimization and Scale-Up of Enzymatic Hydrolysis of Wood Pulp for Cellulosic Sugar Production

Hu¹,

Zhu²,

Fang³

et al. 2016

BioResources

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With the decreased demand for pulp and paper worldwide, the reorganization of pulp and paper mills for cellulosic sugar production is possible. To maximize cellulosic sugar production from the wood pulp with minimum resources, the effects of pH, buffer system, temperature, enzyme loadings, pulp concentrations, and mixing modes on enzymatic hydrolysis were investigated, one factor at a time. Temperature played an important role in enzymatic hydrolysis. When the temperature was lower than 45 °C, the sugar production declined dramatically to almost half of the maximum value. Increasing enzyme dosage, increasing pulp concentration, and adding xylanase increased sugar production. The intermittent manual mixing mode generated higher concentrations of sugars and could be used for large-scale production. At pilot-scale, the diverted pulp for the pulping process was directly hydrolyzed without any treatment, and the residue after hydrolysis was hydrolyzed by adding fresh enzymes. This study provides insight on economically feasible enzymatic hydrolysis of wood pulp at large-scale cellulosic sugar production.

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Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover

Cited by 205 publications

References 38 publications

Establishment of a Simple Lactobacillus plantarum Cell Consortium for Cellulase-Xylanase Synergistic Interactions

Establishment of a Simple Lactobacillus plantarum Cell Consortium for Cellulase-Xylanase Synergistic Interactions

Optimization of a synthetic mixture composed of major Trichoderma reesei enzymes for the hydrolysis of steam-exploded wheat straw

Optimization and Scale-Up of Enzymatic Hydrolysis of Wood Pulp for Cellulosic Sugar Production

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