Supplementation with NAD+ and Its Precursors to Prevent Cognitive Decline across Disease Contexts

Campbell, Jared M.

doi:10.3390/nu14153231

Cited by 21 publications

(12 citation statements)

References 61 publications

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“…Hence, it may be conceivable to use the extracellular NAD present at the target site of some tumors for activation of the enzyme for delivery of a potent CPP-drug, thereby adding an additional level of safety to avoid off-target effects. On another note, since it has been often described that NAD levels decline with age, supplementation with NAD and its precursors is a currently debated topic to counteract age-related diseases such as Alzheimer’s, but also diabetes, , which may be a considerable option to boost NAD levels.…”

Section: Discussionmentioning

confidence: 99%

“…39 Hence, it may be conceivable to use the extracellular NAD present at the target site of some tumors for activation of the enzyme for delivery of a potent CPP-drug, thereby adding an additional level of safety to avoid off-target effects. On another note, since it has been often described that NAD levels decline with age, supplementation with NAD and its precursors is a currently debated topic to counteract age-related diseases such as Alzheimer's, but also diabetes, 40,41 which may be a considerable option to boost NAD levels. For cryptophycin directly conjugated to L17E we observed potency in the nanomolar range, comparable to free cryptophycin in the modified toxicity assay where the compounds were washed from the cells after 2 h of incubation, rather than left on the cells for 72 h. This explains the difference from the low three-digit picomolar toxicity value expected of solitary cryptophycin.…”

Section: ■ Conclusionmentioning

confidence: 99%

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Conditional Cell Penetration of Masked CPPs by an ADEPT-like Approach

Hofmann,

Dombrowsky,

Happel

et al. 2024

ACS Chem. Biol.

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The intracellular delivery of cargos via cell penetrating peptides (CPPs) holds significant promise as a drug delivery vehicle, but a major issue is their lack of cell type specificity, which can lead to detrimental off-target effects. We use an ADEPT-like concept to introduce conditional and selective activation of cellular uptake by using the lysine-rich, cationic, and amphiphilic L17E peptide as a model CPP. By masking the lysine residues of the L17E peptide with enzyme-cleavable acetyl protecting groups, the delivery of the covalently conjugated fluorophore TAMRA to HeLa cells was diminished. Recovery of cellular uptake could be achieved by deacetylation of the masked acetylated L17E peptide using the NAD-dependent sirtuin 2 (SirT2) deacetylase in vitro. Finally, trastuzumab-SirT2 and anti-B7H3-SirT2 antibody-enzyme conjugates were generated for the conditional and selective delivery of a cryptophycin cytotoxin by the L17E peptide. While the masked peptide still demonstrated some cytotoxicity, selective cell killing mediated by the antibodyenzyme conjugates was observed.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Conclusionmentioning

confidence: 99%

Conditional Cell Penetration of Masked CPPs by an ADEPT-like Approach

Hofmann,

Dombrowsky,

Happel

et al. 2024

ACS Chem. Biol.

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“…Still, it emphasized the need for controlled clinical research to determine the efficacy of NAD (+) precursor supplementation in addressing cognitive health in humans. [ 12 ]…”

Section: Lifestyle-based Interventionsmentioning

confidence: 99%

The importance of behavioral interventions in traumatic brain injury

Buccilli,

Alan,

Baha’

et al. 2024

Surgical Neurology International

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Background: Traumatic brain injury (TBI) poses a significant public health concern, profoundly impacting individuals and society. In this context, behavioral interventions have gained prominence as crucial elements in TBI management, addressing the diverse needs of TBI-affected individuals. Methods: A comprehensive literature search was conducted, utilizing databases such as PubMed, Embase, and Scopus. Inclusion criteria encompassed studies focusing on behavioral interventions in TBI, with a particular emphasis on their impact on outcomes. Relevant articles published within the past decade were prioritized, and a qualitative synthesis of the findings was performed. Results: Behavioral interventions have demonstrated their effectiveness in addressing various aspects of TBI care. They have been instrumental in improving cognitive functions, emotional stability, and adaptive behaviors among TBI patients. However, it is important to acknowledge that challenges still exist, including issues related to clinical heterogeneity and healthcare disparities. Conclusion: The integration of behavioral interventions into standard clinical practice marks a transformative shift in TBI care. This approach holds immense potential for enhancing patient outcomes and elevating the overall quality of life for individuals grappling with the complexities of this condition. This review serves as a clarion call for healthcare practitioners, researchers, and policymakers to recognize the pivotal role of behavioral interventions in TBI care, advocating for their wider adoption to advance the field toward a more holistic and patient-centric approach.

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“…Elevated NAD + in the brain can help improve cognitive dysfunction [ 39 ]. In a 2021 study of NMN to improve CICI-induced cognitive dysfunction, it was found that feeding C57 mice at a dose of 250 mg/kg body weight increased their NAD + levels in the hippocampus [ 40 ].…”

Section: Oral Nmn and Exercise Enhance Nad + In Vivomentioning

confidence: 99%

Effect of Exercise and Oral Niacinamide Mononucleotide on Improving Mitochondrial Autophagy in Alzheimer’s Disease

Yuan

Tang

et al. 2023

Nutrients

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Oral niacinamide mononucleotide (NMN) and aerobic exercise have been shown to enhance niacinamide adenine dinucleotide (NAD+) in the body. NAD+ plays a critical role in the body and can directly and indirectly affect many key cellular functions, including metabolic pathways, DNA repair, chromatin remodeling, cell aging, and immune cell function. It is noteworthy that the level of NAD+ decreases gradually with increasing age. Decreased levels of NAD+ have been causally associated with a number of diseases associated with aging, including cognitive decline, cancer, metabolic diseases, sarcopenia, and frailty. Many diseases related to aging can be slowed down or even reversed by restoring NAD+ levels. For example, oral NMN or exercise to increase NAD+ levels in APP/PS1 mice have been proven to improve mitochondrial autophagy, but currently, there is no regimen combining oral NMN with exercise. This review summarizes recent studies on the effect of oral NMN on the enhancement of NAD+ in vivo and the improvements in mitochondrial autophagy abnormalities in AD through aerobic exercise, focusing on (1) how oral NMN improves the internal NAD+ level; (2) how exercise regulates the content of NAD+ in the body; (3) the relationship between exercise activation of NAD+ and AMPK; (4) how SIRT1 is regulated by NAD+ and AMPK and activates PGC-1α to mediate mitochondrial autophagy through changes in mitochondrial dynamics. By summarizing the results of the above four aspects, and combined with the synthesis of NAD+ in vivo, we can infer how exercise elevates the level of NAD+ in vivo to mediate mitochondrial autophagy, so as to propose a new hypothesis that exercise interferes with Alzheimer’s disease (AD).

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Supplementation with NAD+ and Its Precursors to Prevent Cognitive Decline across Disease Contexts

Cited by 21 publications

References 61 publications

Conditional Cell Penetration of Masked CPPs by an ADEPT-like Approach

Conditional Cell Penetration of Masked CPPs by an ADEPT-like Approach

The importance of behavioral interventions in traumatic brain injury

Effect of Exercise and Oral Niacinamide Mononucleotide on Improving Mitochondrial Autophagy in Alzheimer’s Disease

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