Supplementation with Extracellular Vesicles Derived from Adipose-Derived Stem Cells Increases Fat Graft Survival and Browning in Mice: A Cell-Free Approach to Construct Beige Fat from White Fat Grafting

Laloze, Jérôme; Lupon, Elise; Girard, Paul; Gandolfi, Silvia; Fiévet, Loïc; Desmoulière, Alexis

doi:10.1097/prs.0000000000007821

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…90 By contrast, the uptake of adipose stem cell-derived extracellular vesicles by macrophages promotes M2-type polarization and catecholamine secretion, while also inhibiting M1-type polarization and reducing necrosis and fibrosis in damaged adipose tissue. 91 Recent studies of lipid metabolism in macrophages have shown that the uptake of oxidized low-density lipoprotein (OX-LDL) and cholesterol by macrophages is principally mediated by scavenger receptors (SCARB1, CD36, and macrophage scavenger receptor 1 [MSR1]).…”

Section: Removal Of Dead Adipocytes and Metabolism Of Extracellular L...mentioning

confidence: 99%

“…Paracrine activity of proinflammatory cytokines and chemokines, 88 including IFNγ, TNFα, and TGFβ, is involved in the formation of foam cells, 89 which also release proinflammatory cytokines, such as TNF, IL‐1, and IL‐6, and chemokines, such as CCL5 and chemokine ligand 1 (CXCL1) 90 . By contrast, the uptake of adipose stem cell‐derived extracellular vesicles by macrophages promotes M2‐type polarization and catecholamine secretion, while also inhibiting M1‐type polarization and reducing necrosis and fibrosis in damaged adipose tissue 91 …”

Section: Effects Of Macrophages On Metabolic Regulation In Adipose Ti...mentioning

confidence: 99%

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Inflammation‐mediated metabolic regulation in adipose tissue

Xu,

Lu,

Gao

et al. 2024

Obesity Reviews

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SummaryChronic inflammation of adipose tissue is a prominent characteristic of many metabolic diseases. Lipid metabolism in adipose tissue is consistently dysregulated during inflammation, which is characterized by substantial infiltration by proinflammatory cells and high cytokine concentrations. Adipose tissue inflammation is caused by a variety of endogenous factors, such as mitochondrial dysfunction, reactive oxygen species (ROS) production, endoplasmic reticulum (ER) stress, cellular senescence, ceramides biosynthesis and mediators of lipopolysaccharides (LPS) signaling. Additionally, the gut microbiota also plays a crucial role in regulating adipose tissue inflammation. Essentially, adipose tissue inflammation arises from an imbalance in adipocyte metabolism and the regulation of immune cells. Specific inflammatory signals, including nuclear factor‐κB (NF‐κB) signaling, inflammasome signaling and inflammation‐mediated autophagy, have been shown to be involved in the metabolic regulation. The pathogenesis of metabolic diseases characterized by chronic inflammation (obesity, insulin resistance, atherosclerosis and nonalcoholic fatty liver disease [NAFLD]) and recent research regarding potential therapeutic targets for these conditions are also discussed in this review.

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Section: Removal Of Dead Adipocytes and Metabolism Of Extracellular L...mentioning

confidence: 99%

Section: Effects Of Macrophages On Metabolic Regulation In Adipose Ti...mentioning

confidence: 99%

Inflammation‐mediated metabolic regulation in adipose tissue

Xu,

Lu,

Gao

et al. 2024

Obesity Reviews

View full text Add to dashboard Cite

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Exosomes with overexpressed miR 147a suppress angiogenesis and infammatory injury in an experimental model of atopic dermatitis

Shi

Pei

Ding

et al. 2023

Sci Rep

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Atopic dermatitis is defined as an intensely systemic inflammation among skin diseases. Exosomes derived from adipose-derived stem cells may be a novel cell-free therapeutic strategy for atopic dermatitis treatment. This study aims to elucidate the possible underlying mechanism of adipose-derived stem cells-exosomes harboring microRNA-147a in atopic dermatitis pathogenesis. BALB/c mice treated with Dermatophagoides farinae extract/2,4-dinitrochlorobenzene were defined as a mouse model of atopic dermatitis, either with inflamed HaCaT cells and HUVECs exposed with TNF-α/IFN-γ stimulation were applied for a cell model of atopic dermatitis. The concentrations of IL-1β and TNF-α in the supernatants were examined by ELISA. Cell viability and migration were assessed by MTT and Transwell assay. The apoptosis was examined using flow cytometry and TUNEL staining. The tube formation assay was employed to analyzed angiogenesis. The molecular regulations among miR-147a, MEF2A, TSLP and VEGFA were confirmed using luciferase reporter assay, either with ChIP. microRNA-147a was markedly downregulated in the serum and skin samples of atopic dermatitis mice, of which overexpression remarkably promoted HaCaT cell proliferation, meanwhile inhibiting inflammatory response and cell apoptosis. microRNA-147a in adipose-derived stem cells was subsequently overexpressed, and exosomes (Exos-miR-147a mimics) were collected. Functionally, exos-microRNA-147a mimics attenuated TNF-α/IFN-γ-induced HaCaT cell inflammatory response and apoptosis, and suppressed HUVECs angiogenesis. Encouraging, molecular interaction experiments revealed that exosomal microRNA-147a suppressed TNF-α/IFN-γ-induced HUVECs angiogenesis by targeting VEGFA, and exosomal microRNA-147a repressed HaCaT cells inflammatory injury through the MEF2A-TSLP axis. Mechanistically, exosomal microRNA-147a repressed pathological angiogenesis and inflammatory injury during atopic dermatitis progression by targeting VEGFA and MEF2A-TSLP axis. microRNA-147a-overexpressing adipose-derived stem cells-derived exosomes suppressed pathological angiogenesis and inflammatory injury in atopic dermatitis by targeting VEGFA and MEF2A-TSLP axis.

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Supplementation with Extracellular Vesicles Derived from Adipose-Derived Stem Cells Increases Fat Graft Survival and Browning in Mice: A Cell-Free Approach to Construct Beige Fat from White Fat Grafting

Cited by 2 publications

References 5 publications

Inflammation‐mediated metabolic regulation in adipose tissue

Inflammation‐mediated metabolic regulation in adipose tissue

Exosomes with overexpressed miR 147a suppress angiogenesis and infammatory injury in an experimental model of atopic dermatitis

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