Supplementation with Docosahexaenoic Acid and Vitamin E Improves Hepatic Triglyceride Accumulation Induced by High‐Fat Diet in Mice

Abstract

The study aims to investigate the effect of combined supplementation with docosahexaenoic acid (C22:6 n‐3, DHA) and vitamin E (VE) on the oxidative stress and liver triglycerides (TG) accumulation induced by high‐fat diet (HFD) in mice. C57BL/6J mice are fed either a control diet or an HFD for 8 weeks. Animals are supplemented with DHA, VE, or DHA + VE, respectively. Supplementation with DHA alone shows significant improvement in oxidative stress and hepatic steatosis in mice. Supplementation with DHA significantly reduces the liver TG and total cholesterol contents, and the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, compared with the HFD. Supplementation with DHA also significantly decreases the mRNA expression level of sterol regulatory element‐binding protein 1C. However, supplementation with VE alone does not show improvement in oxidative stress and hepatic steatosis. DHA + VE supply obtains a superior effect in alleviation of hepatic steatosis than DHA supplementation alone in mice fed by HFD. The efficacy of DHA potentiated by VE can be due to that VE enhances the effect of DHA in decrease of ALT and AST levels and increase of antioxidant enzyme activity and glutathione level in mice fed by HFD. Practical Applications: Supplementation with DHA significantly improves the oxidative stress and hepatic steatosis induced by HFD in mice. The efficacy of DHA in the alleviation of hepatic steatosis induced by HFD is potentiated by VE. These findings may provide a rational basis for the use of DHA and VE co‐supplementation in patients with liver steatosis.

DHA-enriched phosphatidylcholine from Clupea harengus roes regulates the gut–liver axis to ameliorate high-fat diet-induced non-alcoholic fatty liver disease

“…DHA and soybean PC effectively reduced the HFD-induced ALT and AST murine levels and alleviated hepatic steatosis. 25,43 In this study, DHA-PC treatment effectively decreased the activities of these two enzymes in serum. Moreover, H&E stains indicated that DHA-PC treatment alleviated HFD-induced liver damage.…”

“…54,55 At the phylum level, the relative abundance of Actinobacteriota increased, Bacteroidetes decreased, and the F/B ratio increased in the HFD mice. 43,56 The abundance of Actinobacteriota was significantly elevated in the NAFLD patients, and its abundance is closely associated with the degree of both fatty liver and abnormal liver function. 57 The increase in the relative abundance of Actinobacteriota may contribute to the development of mild NAFLD-induced by HFD -into NASH.…”

The Effect of Dietary Protein Hydrolysate from Black Soldier Fly Larvae and Schizochytrium on Palatability, Nutrient Metabolites and Health Status in Beagle Dogs

“…Similar to our results, the addition of BSFL meal led to a reduction in AST and ALT for African catfish [ 33 ]. Moreover, many studies have demonstrated that DHA also reduces high-fat-diet induced AST and ALT contents [ 38 ] and alleviates bisphenol (BPA)-induced nephrotoxicity by decreasing BUN and CERA concentrations in plasma [ 39 ].…”

Influence of a Mixture of Protein Hydrolysate from Black Soldier Fly Larvae and Schizochytrium on Palatability, Plasma Biochemistry, and Antioxidative and Anti-Inflammatory Capacity in Cat Diets

“…The content of enzyme activities, such as AST and ALT, and the content of CREA and BUN are related to the health of the liver and kidneys. Early research demonstrated that DHA effectively reduced the high-fat-diet-induced ALT and AST content and alleviated hepatic disease [ 41 ], and DHA can reduce BPA-induced nephrotoxicity by decreasing BUN and CERA concentrations in plasma [ 42 ]. However, the initial blood biochemistry parameters of the cats were not measured.…”