Supplementation of kaempferol to<i>in vitro</i>maturation medium regulates oxidative stress and enhances subsequent embryonic development<i>in vitro</i>

Zhao, Yuhan; Xu, Yong‐Nan; Li, Yinghua; Jin, Qingguo; Sun, Jingyu; Zhiqiang, E; Gao, Qingshan

doi:10.1017/s0967199419000674

Cited by 18 publications

(13 citation statements)

References 34 publications

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“…Caspase-3 is an important mediator of apoptosis (Ohnishi et al, 2019) and KAE has been proven to reduce apoptosis in early porcine embryos by blocking caspase-3 expression (Y. Zhao et al, 2020). Among genes regulating apoptosis, Bax and Bcl-2 are the most important.…”

Section: Discussionmentioning

confidence: 99%

Supplementation with kaempferol relieves oxidative stress and enhances development of early bovine embryos in vitro

Zhao

Ding

Wang

et al. 2022

Reprod Domestic Animals

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Oxidative stress (OS) has been considered the principle cause of developmental failure of early embryos cultured in vitro; therefore, the addition of antioxidants is very important for improving in vitro culture (IVC) systems. Various antioxidants have been tested for IVC systems, but most have exhibited some side effects. Kaempferol (3,5,7‐trihydroxy‐2‐[4‐hydroxyphenyl]‐4 h‐1‐benzopyran‐4‐one, KAE) is a flavonoid with strong antioxidant activity and no obvious side effects. This study explored the effect of KAE on antioxidant capacity and developmental competence of bovine embryos after fertilization. KAE was added to bovine IVC medium and significantly reduced reactive oxygen species (ROS) in 2‐, 4‐ and 8‐cell stage embryos and increased blastocyst formation. In addition, the level of H3K9ac was increased, the apoptotic index was reduced and total cell numbers and trophectoderm cell numbers in day 7 blastocysts were increased significantly in KAE‐treated embryos compared to control. Expression of the apoptotic gene, Bcl‐2, was higher in blastocysts after KAE treatment, while expression of the endoplasmic reticulum (ER) stress genes, Bip and HDAC1, and the pro‐apoptotic gene, Bax, were significantly lower in the KAE group. Thus, KAE significantly reduced ROS damage and improved development of IVC bovine embryos.

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Section: Discussionmentioning

confidence: 99%

Supplementation with kaempferol relieves oxidative stress and enhances development of early bovine embryos in vitro

Zhao

Ding

Wang

et al. 2022

Reprod Domestic Animals

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“…Flavonoids are polyphenols that protect plant cells against microorganisms, insects, and UV irradiation. Previous studies have shown that flavonoids such as kaempferol and quercetin exert antioxidant effects on mammalian oocytes ( Cao et al, 2020 ; Zhao et al, 2020 ). Lut, a flavone in the flavonoid group, also exhibits antioxidant activity.…”

Section: Discussionmentioning

confidence: 99%

Luteolin Orchestrates Porcine Oocyte Meiotic Progression by Maintaining Organelle Dynamics Under Oxidative Stress

Park

Jeong

Joo

et al. 2021

Front. Cell Dev. Biol.

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Increasing evidence has demonstrated that oxidative stress impairs oocyte maturation, but the underlying mechanisms remain largely unknown. Here, for the first time, we examined the antioxidant role of luteolin in meiotic progression and the underlying mechanisms. Supplementation of 5 μM luteolin increased the rates of first polar body extrusion and blastocyst formation after parthenogenetic activation, and the expression levels of oocyte competence (BMP15 and GDF9)-, mitogen-activated protein kinase (MOS)-, and maturation promoting factor (CDK1 and Cyclin B)-related genes were also improved. Luteolin supplementation decreased intracellular reactive oxygen species levels and increased the expression levels of oxidative stress-related genes (SOD1, SOD2, and CAT). Interestingly, luteolin alleviated defects in cell organelles, including actin filaments, the spindle, mitochondria, the endoplasmic reticulum, and cortical granules, caused by H2O2 exposure. Moreover, luteolin significantly improved the developmental competence of in vitro-fertilized embryos in terms of the cleavage rate, blastocyst formation rate, cell number, cellular survival rate, and gene expression and markedly restored the competencies decreased by H2O2 treatment. These findings revealed that luteolin supplementation during in vitro maturation improves porcine meiotic progression and subsequent embryonic development by protecting various organelle dynamics against oxidative stress, potentially increasing our understanding of the underlying mechanisms governing the relationship between oxidative stress and the meiotic events required for successful oocyte maturation.

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“…For instance, at a concentration of 10 μM, it could enhance the primordial follicle activation, cell proliferation and oocyte meiotic resumption in the ovine cultured follicles (Santos et al, 2019). Similarly, at 0.1μM concentration, KAE was effective in increasing the blastocyst number as well as its formation rate in porcine cultured follicles (Zhao et al, 2019). Likewise, at 1µM concentration KAE was proven to be an effective agent by increasing the follicle diameter and oocyte growth in mammals (Zhou et al, 2015).…”

Section: Bioactive Compounds Kaempferolmentioning

confidence: 96%

“…Through this pathway, KAE exerts its effects on the culture of ovine preantral follicles in-vitro (Santos et al, 2019). Besides this, another mechanism of KAE is to increase the mRNA expression levels of COX2 and SOX2 genes which have a role in embryonic developmentwith a significant reduction in the Caspase-3 levels (Zhao et al, 2019). This further, helps in the improvement of zygote development in porcine cultures (W hite et al, 2016).…”

Section: Bioactive Compounds Kaempferolmentioning

confidence: 99%

Molecular Targets of Phyto-bioactive Compounds in Female Reproductive System of Mammals: A Review

Kumar¹,

Manasa²,

Ramesh³

et al. 2021

IJAR

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Phytochemicals present in the plants are divided into primary (Alcohol Amino acids, nucleotides. etc) and secondary metabolites (Alkaloids, Saponins etc.). Carotenoids (reduces reactive oxygen species formation, decreases apoptotic cells, restoration of actin capping expression proteins etc.), Phenolics (inhibits extracellular signal-regulated kinase signalling pathway), Isoflavones (inhibits tyrosine kinase pathway) and alkaloids (downregulation of vascular endothelial growth factor, tumor necrosis factor-alpha and hypoxia-inducible factor 1-alpha messengers) are the major phytochemicals, having the potential effects towards ovarian function. Likewise, bioactive compounds are the chemicals that can interact with certain components of live tissue to exert their various effects (antioxidant, antineoplastic, receptor inhibition, gene expression etc.) respective to female fertility. Similarly, bioactive compounds: Kaempferol [phosphatidylinositol -3- kinase (PI3K)/protein kinase B (Akt) pathway], Quercetin (controlling the release of 17β-estradiol etc.), Myricetin (PI3K/Akt and MAPK signalling pathway), Galngin (inhibition of angiogenesis via decreasing the VEGF and p-Akt) and Resveratrol (regulation of Foxo3a and SIRT1 genes etc.) shows its effects by targeting different molecules and/or pathways at the ovarian microenvironment. However, Genistein (binding to estrogen receptors: ESRα and ESRβ etc.) and Diadzein (disrupting the endocrines etc.) emphatically interfere with the ovarian functions. Besides this, molecular effects exerted by these phyto-bioactive compounds on the in vivo and/or in vitro ovarian culture systems entirely depend on their dosage: Kaempferol @10 μM increased the primordial follicle activation, Quercetin @4 µM improved the quality of oocytes whereas @8 µM reduced the quality), Resveratrol @ 2 µM increased the blastocyst formation, Myricetin @ 100 mg/kg/day feeding in rats induced estrogenic activity, Genistein, feeding in female mice @ 500 and 1000ppm increased the gestation time and Diazdein causes the inhibition of 3-hydroxysteroid dehydrogenase at 40 µM doses. The assessment was done via the systemic collection of literature from sources such as newspapers, conference papers, journals, theory and dissertation articles, electronic databases, manuals, encyclopedia and annual reviews, as well as e-books and reporting. As a result, the preceding discussion focuses on the key phyto-bioactive compounds and their molecular targets in female fertility. This will aid in the successful and secure application of plant bioactive compounds in the field of female reproductive health.

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Supplementation of kaempferol toin vitromaturation medium regulates oxidative stress and enhances subsequent embryonic developmentin vitro

Cited by 18 publications

References 34 publications

Supplementation with kaempferol relieves oxidative stress and enhances development of early bovine embryos in vitro

Supplementation with kaempferol relieves oxidative stress and enhances development of early bovine embryos in vitro

Luteolin Orchestrates Porcine Oocyte Meiotic Progression by Maintaining Organelle Dynamics Under Oxidative Stress

Molecular Targets of Phyto-bioactive Compounds in Female Reproductive System of Mammals: A Review

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