Supplementation of freezing medium with encapsulated or free glutathione during cryopreservation of bull sperm

Nadri, Touba; Towhidi, Armin; Zeinoaldini, S.; Riazi, Gholam Hossein; Sharafi, Mohsen; Zhandi, Mahdi; Kastelic, John P.; Gholami, Dariush

doi:10.1111/rda.14088

Cited by 3 publications

(2 citation statements)

References 44 publications

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“…Lorenzo-Torres et al [48] reported 122 nuclei in good-quality blastocysts produced by IVF. These results are lower than those obtained in the present study, but they are similar to those reported by Agata et al [49], Mastrorocco et al [50], and Nadri et al [51]. In the present study, before vitrification, treatments with 0.25 and 0.5 µM of resveratrol were statistically similar between them, although 0.5 µM showed the lowest number of nuclei, with 1 µM being the treatment that gave the highest number of nuclei in early blastocysts.…”

Section: Effect Of Resveratrol On Embryo Qualitysupporting

confidence: 85%

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

González-Garzón,

Ramón-Ugalde,

Ambríz-García

et al. 2023

Animals

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The in vitro production and cryopreservation of mammalian embryos generates reactive oxygen species (ROS) due to conditions of the system that can overcome their antioxidant protection. Resveratrol is an antioxidant used in in vitro systems to improve blastocyst rates, but its effect on antioxidant enzymes such as glutathione (GSH) in embryos produced by in vitro fertilization (IVF) after vitrification has not been reported. The objective of this study was to evaluate the effects of resveratrol on the in vitro maturation medium (IVM) of sheep oocytes (Ovis aries) on the levels of ROS and GSH in embryos produced by IVF subjected to vitrification. Resveratrol was added at 0 µM, 0.25 µM, 0.5 µM, and 1 µM during oocyte in vitro maturation (IVM). Matured oocytes were fertilized with thawed ram sperm. Embryos were cultured in sequential media until blastocysts, were then vitrified for 24 h, and, after heating, they were stained with DCFH-DA (2′,7′-dichlorodihydrofluorescein diacetate) to determine the presence of ROS and with Cell Tracker Blue® for the presence of GSH. The quantitative values of ROS and GSH were obtained through the Image J image processor. The results showed that resveratrol increased GSH and decreased ROS production (p < 0.05) in a dose-dependent manner. It is concluded that its use in sheep oocytes during IVM has a beneficial effect on embryos produced by IVF subjected to vitrification by decreasing reactive oxygen species levels and facilitating the generation of embryo antioxidant enzymes like glutathione.

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Section: Effect Of Resveratrol On Embryo Qualitysupporting

confidence: 85%

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

González-Garzón,

Ramón-Ugalde,

Ambríz-García

et al. 2023

Animals

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“…Before semen cryopreservation, six semen samples were individually split into 4 equals and diluted to a final concentration of 6.0 × 10 7 /ml spermatozoa with the basic extender supplemented with 0 μg/ml SeNPs and 0 mM GSH (negative control group, NC), 2 μg/ml SeNPs (SeNPs group) ( 27 ), 4 mM GSH (GSH group) ( 50 ) and 4 mM GSH plus 2 μg/ml SeNPs (GSH + SeNPs group). Dose selections for GSH were conducted according to previous publications ( 5 , 56 ).…”

Section: Methodsmentioning

confidence: 99%

Glutathione and selenium nanoparticles have a synergistic protective effect during cryopreservation of bull semen

Ren

Zhang

et al. 2023

Front. Vet. Sci.

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IntroductionIn the present study, the synergistic protective effect of co-supplementation of glutathione (GSH) with selenium nanoparticles (SeNPs) on the cryopreservation efficiency of bull semen was analyzed.MethodsAfter collection, the ejaculates of Holstein bulls were subsequently diluted with a Tris extender buffer supplemented with different concentrations of SeNPs (0, 1, 2, and 4 μg/ml), followed by semen equilibration at 4°C and assessment of sperm viability and motility. Subsequently, the ejaculates of Holstein bulls were pooled, split into four equal groups, and diluted with a Tris extender buffer supplemented with basic extender (negative control group, NC group), 2 μg/ml SeNPs (SeNPs group), 4 mM GSH (GSH group), and 4 mM GSH plus 2 μg/ml SeNPs (GSH + SeNPs group). After cryopreservation, motility, viability, mitochondrial activity, plasma membrane integrity, acrosome integrity, concentration of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT), and ability of frozen-thawed sperm cells to support in vitro embryonic development were evaluated.Results and discussionNo side effect of SeNPs concentrations applied in the current study on the motility and viability of equilibrated bull spermatozoa was found. Meanwhile, supplementation of SeNPs significantly promoted the motility and viability of equilibrated bull spermatozoa. Furthermore, the co-supplementation of GSH with SeNPs effectively protected bull spermatozoa from cryoinjury as expressed by promoting semen motility, viability, mitochondrial activity, plasma membrane integrity, and acrosome integrity. Finally, the enhanced antioxidant capacity and embryonic development potential in the frozen-thawed bull spermatozoa cryopreserved by co-supplementation of GSH with SeNPs further confirmed the synergistic protective effect of co-supplementation of GSH with SeNPs on the cryopreservation of bull semen.

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Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

González-Garzón,

Ramón-Ugalde,

Ambríz-García

et al. 2023

Preprint

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The in vitro production and cryopreservation of mammalian embryos generate reactive oxygen species (ROS) due to conditions of the system that can overcome their antioxidant protection. Resveratrol is an antioxidant used in in vitro systems improving blastocyst rates, but its effect on antioxidant enzymes such as glutathione (GSH) in embryos produced by in vitro fertilization (IVF) after vitrification has not been reported. The objective of this study was to evaluate the effect of resveratrol in the in vitro maturation medium (IVM) of sheep oocytes (Ovis aries) on the levels of ROS and GSH in embryos produced by IVF subjected to vitrification. Resveratrol was added at 0µM, 0.25µM, 0.5µM and 1µM during oocyte in vitro maturation (IVM). Matured oocytes were fertilized with thawed ram sperm. Embryos were cultured in sequential media until blastocysts, then were vitrified for 24 h and after heating they were stained with DCFH-DA (2',7'-dichlorodihydrofluorescein diacetate) to determine the presence of ROS and with Cell Tracker Blue® for the presence of GSH. The quantitative values of ROS and GSH were obtained through the Image J image processor. The results showed that resveratrol increases GSH and decreases ROS production (p<0.05). It is concluded that its use in sheep oocytes during IVM has a beneficial effect on embryos produced by IVF subjected to vitrification, protecting them from oxidative damage by promoting the synthesis of antioxidants.

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Supplementation of freezing medium with encapsulated or free glutathione during cryopreservation of bull sperm

Cited by 3 publications

References 44 publications

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

Glutathione and selenium nanoparticles have a synergistic protective effect during cryopreservation of bull semen

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

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